Abstract
A total of 137 strains of Staphylococcus species were blindly tested by four rapid serological assays, and the results were compared with those of the tube coagulase assay. For the S. aureus isolates, the Sero-STAT Staph assay gave six false-negative results, four of which were for methicillin-resistant strains. The Accu -Staph, Staphylatex , and Staphyloslide assays identified all the coagulase-positive strains as Staphylococcus aureus. Among the coagulase-negative staphylococci, false-positive results were seen with strains of S. capitis. S. saprophyticus, and S. cohnii. The overall accuracy of the kits compared with the tube coagulase test ranged from 95.1 to 100%.
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- Aldridge K. E., Stratton C. W., Patterson L. S., Evans M. E., Hodges R. L. Comparison of the Staph-Ident system with a conventional method for species identification of urine and blood isolates of coagulase-negative staphylococci. J Clin Microbiol. 1983 Mar;17(3):516–520. doi: 10.1128/jcm.17.3.516-520.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barry A. L., Badal R. E. Reliability of the microdilution technic for detection of methicillin-resistant strains of staphylococcus aureus. Am J Clin Pathol. 1977 May;67(5):489–495. doi: 10.1093/ajcp/67.5.489. [DOI] [PubMed] [Google Scholar]
- Doern G. V. Evaluation of a commercial latex agglutination test for identification of Staphylococcus aureus. J Clin Microbiol. 1982 Mar;15(3):416–418. doi: 10.1128/jcm.15.3.416-418.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Essers L., Radebold K. Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980 Nov;12(5):641–643. doi: 10.1128/jcm.12.5.641-643.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gramoli J. L., Wilkinson B. J. Characterization and identification of coagulase-negative, heat-stable deoxyribonuclease-positive staphylococci. J Gen Microbiol. 1978 Apr;105(2):275–285. doi: 10.1099/00221287-105-2-275. [DOI] [PubMed] [Google Scholar]
- Lachica R. V., Genigeorgis C., Hoeprich P. D. Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity. Appl Microbiol. 1971 Apr;21(4):585–587. doi: 10.1128/am.21.4.585-587.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MOSSEL D. A. Attempt in classification of catalase-positive staphylococci and micrococci. J Bacteriol. 1962 Dec;84:1140–1147. doi: 10.1128/jb.84.6.1140-1147.1962. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Myrick B. A., Ellner P. D. Evaluation of the latex slide agglutination test for identification of Staphylococcus aureus. J Clin Microbiol. 1982 Feb;15(2):275–277. doi: 10.1128/jcm.15.2.275-277.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Orth D. S., Chugg L. R., Anderson A. W. Comparison of animal sera for suitability in coagulase testing. Appl Microbiol. 1971 Mar;21(3):420–425. doi: 10.1128/am.21.3.420-425.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sperber W. H., Tatini S. R. Interpretation of the tube coagulase test for identification of Staphylococcus aureus. Appl Microbiol. 1975 Apr;29(4):502–505. doi: 10.1128/am.29.4.502-505.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Winblad S., Ericson C. Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Methodology and epidemiology with special reference to weakly reacting methicillin-resistant strains. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Feb;81(1):150–156. [PubMed] [Google Scholar]
- Yrios J. W. Comparison of rabbit and pig plasma in the tube coagulase test. J Clin Microbiol. 1977 Feb;5(2):221–224. doi: 10.1128/jcm.5.2.221-224.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zarzour J. Y., Belle E. A. Evaluation of three test procedures for identification of Staphylococcus aureus from clinical sources. J Clin Microbiol. 1978 Feb;7(2):133–136. doi: 10.1128/jcm.7.2.133-136.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]