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. 2009 Jul;127(3):326–337. doi: 10.1111/j.1365-2567.2008.02979.x

Figure 2.

Figure 2

Soluble egg antigen (SEA) selectively up-regulates Jagged-1 mRNA and protein expression in mouse and human macrophages. Bone marrow macrophages (BMMs) [plated overnight without colony-stimulating factor (CSF)-1] were treated for 2 hr with 10 ng/ml lipopolysaccharide (LPS), 10 μg/ml SEA or medium as a control (a, b, c), or treated for 2 hr with various concentrations of LPS or SEA (d). Human monocyte-derived macrophages (HMDMs) were treated for 2, 4 and 7 hr (f). Gene expression was calculated by real-time polymerase chain reaction (PCR) for Jagged-1 (a, d, f), interleukin (IL)-33 (b) and IL-12p40 (c). For quantification of protein expression, BMMs (e) or HMDMs (g) were plated overnight in the absence of CSF-1 and treated for 4 hr with 10 ng/ml LPS, 10 μg/ml SEA or medium as a control. Total Jagged-1 and extracellular signal-regulated kinase-1/2 (ERK-1/2) protein were visualized by immunoblotting. Results for the mouse experiments are from six independent experiments, displayed as mean + SEM (a, b, c), or are representative of three or more similar experiments (d, e). Results for the human experiments (f, g) are representative of two similar experiments using different donors. *, P < 0·05; **, P < 0·01; #, P = 0·058; NS, not significant for either LPS or SEA versus control treatments.