Abstract
A direct extraction of the antigens of group A beta-hemolytic streptococci from 557 throat swabs was performed by a new microtechnique of the nitrous acid extraction method with the Culturette Brand Ten-Minute Strep ID technique from Marion Scientific, Division of Marion Laboratories, Inc., Kansas City, Mo. This group A latex reagent kit contains the reagents for the micronitrous acid extraction of throat swabs and does not require a centrifugation step in its protocol. There was a 99.3% (553 of 557) total agreement between the direct nitrous acid extraction-latex agglutination method and the standard culture method. The direct extraction method yielded an identification of 95.1% (78 of 82) of the group A streptococci identified by the standard method. Throat swabs used for standard culture may also be extracted with nitrous acid for the detection of group A antigen. A 5-min nitrous acid extraction destroys the viability of bacteria associated with normal throat flora as well as group A streptococci and Mycobacterium tuberculosis. This highly rapid method is simple to perform and requires no costly instrumentation. Accordingly, it would be most applicable in a hospital laboratory as well as in a physician's office.
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Selected References
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- Ederer G. M., Herrmann M. M., Bruce R., Matsen J. M., Chapman S. S. Rapid extraction method with pronase B for grouping beta-hemolytic streptococci. Appl Microbiol. 1972 Feb;23(2):285–288. doi: 10.1128/am.23.2.285-288.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerber M. A. Micronitrous acid extraction-coagglutination test for rapid diagnosis of streptococcal pharyngitis. J Clin Microbiol. 1983 Jan;17(1):170–171. doi: 10.1128/jcm.17.1.170-171.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kholy A. E., Facklam R., Sabri G., Rotta J. Serological identification of group A streptococci from throat scrapings before culture. J Clin Microbiol. 1978 Dec;8(6):725–728. doi: 10.1128/jcm.8.6.725-728.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lue Y. A., Howit I. P., Ellner P. D. Rapid grouping of beta-hemolytic streptococci by latex agglutination. J Clin Microbiol. 1978 Sep;8(3):326–328. doi: 10.1128/jcm.8.3.326-328.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Otero J. R., Reyes S., Noriega A. R. Rapid diagnosis of group A streptococcal antigen extracted directly from swabs by an enzymatic procedure and used to detect pharyngitis. J Clin Microbiol. 1983 Aug;18(2):318–320. doi: 10.1128/jcm.18.2.318-320.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Slifkin M., Freedel D., Gil G. M. Direct serogrouping of group B streptococci from urogenital and gastric swabs with nitrous acid extraction and the Phadebact streptococcus test. Am J Clin Pathol. 1982 Dec;78(6):850–853. doi: 10.1093/ajcp/78.6.850. [DOI] [PubMed] [Google Scholar]
- Slifkin M., Gil G. M. Serogrouping of beta-hemolytic streptococci from throat swabs with nitrous acid extraction and the Phadebact streptococcus test. J Clin Microbiol. 1982 Jan;15(1):187–189. doi: 10.1128/jcm.15.1.187-189.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Watson B. K., Moellering R. C., Jr, Kunz L. J. Identification of streptococci: use of lysozyme and Streptomyces albus filtrate in the preparation of extracts for Lancefield grouping. J Clin Microbiol. 1975 Mar;1(3):274–278. doi: 10.1128/jcm.1.3.274-278.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- el Kholy A., Wannamaker L. W., Krause R. M. Simplified extraction procedure for serological grouping of beta-hemolytic streptococci. Appl Microbiol. 1974 Nov;28(5):836–839. doi: 10.1128/am.28.5.836-839.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]