Abstract
The inhibitory and bactericidal effects of sodium polyanetholesulfonate on Neisseria meningitidis were neutralized by blood lysis which occurs within the pediatric Isolator 1.5 Microbial tube (E. I. du Pont de Nemours & Co., Inc., Wilmington, Del.). Lysed blood was more effective than 2.5% gelatin in preventing the inhibitory effect. All but 1 of 16 N. meningitidis strains were killed by 0.06% sodium polyanetholesulfonate in the absence of lysed blood, whereas none were killed by 1.0% sodium polyanetholesulfonate when lysed blood was present. The possible clinical relevance of these results was reflected in the improved detection of meningococcemia in children when the Isolator 1.5 Microbial tube was compared with a conventional broth system.
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Selected References
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- Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
- Edberg S. C., Edberg M. K. Inactivation of the polyanionic detergent sodium polyanetholsulfonate by hemoglobin. J Clin Microbiol. 1983 Nov;18(5):1047–1050. doi: 10.1128/jcm.18.5.1047-1050.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eng J. Effect of sodium polyanethol sulfonate in blood cultures. J Clin Microbiol. 1975 Feb;1(2):119–123. doi: 10.1128/jcm.1.2.119-123.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eng J., Holten E. Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media. J Clin Microbiol. 1977 Jul;6(1):1–3. doi: 10.1128/jcm.6.1.1-3.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Eng J., Iveland H. Inhibitory effect in vitro of sodium polyanethol sulfonate on the growth of Neisseria meningitidis. J Clin Microbiol. 1975 May;1(5):444–447. doi: 10.1128/jcm.1.5.444-447.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pai C. H., Sorger S. Enhancement of recovery of Neisseria meningitidis by gelatin in blood culture media. J Clin Microbiol. 1981 Jul;14(1):20–23. doi: 10.1128/jcm.14.1.20-23.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Staneck J. L., Vincent S. Inhibition of Neisseria gonorrhoeae by sodium polyanetholesulfonate. J Clin Microbiol. 1981 Mar;13(3):463–467. doi: 10.1128/jcm.13.3.463-467.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Traub W. H., Lowrance B. L. Anticomplementary, anticoagulatory, and serum-protein precipitating activity of sodium polyanetholsulfonate. Appl Microbiol. 1970 Sep;20(3):465–468. doi: 10.1128/am.20.3.465-468.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]