Abstract
A diphasic medium, Trans-Isolate medium, was developed for the transport of primary cultures of cerebrospinal fluids from patients with bacterial meningitis. It consists of a charcoal-starch agar slant and soybean-casein digest-gelatin broth buffered at pH 7.2 with 0.1 M 3-(N-morpholino)propanesulfonic acid buffer. In the laboratory, this medium supported the growth and survival of stock cultures of Neisseria meningitidis, Streptococcus pneumoniae, and Haemophilus influenzae for at least 3 months. Under field conditions in Africa, cerebrospinal fluids from patients suspected of having bacterial meningitis were inoculated directly onto plates of chocolate agar medium and into bottles of Trans-Isolate medium. An etiological agent was isolated from 52 spinal fluids by direct plating. After shipment to Atlanta, Ga., 2 to 4 weeks later, the same etiological agents were recovered from 38 bottles of Trans-Isolate medium.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cadoz M., Denis F., Mar I. D. Etude épidémiologique des cas de méningites purulentes hospitalisés à Dakar pendant la décennie 1970-1979. Bull World Health Organ. 1981;59(4):575–584. [PMC free article] [PubMed] [Google Scholar]
- Hoffman P. S., Pine L., Bell S. Production of superoxide and hydrogen peroxide in medium used to culture Legionella pneumophila: catalytic decomposition by charcoal. Appl Environ Microbiol. 1983 Mar;45(3):784–791. doi: 10.1128/aem.45.3.784-791.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanborn W. R. Meningitis diagnostic bacteriology. J Egypt Public Health Assoc. 1969;44(4):385–407. [PubMed] [Google Scholar]