Figure 4.

Comparative analysis between transcripts increasing in expression during prion disease and unchanging during cuprizone treatment. (A) After SOM clustering analysis, one cluster emerged containing transcripts that increased in expression over the time course of prion infection (shown here, 333 total transcripts). (B) The cuprizone data set was mined for one transcript whose expression was unchanged as compared to control (Tbp). Using the Euclidean similarity metric, transcripts with 100-85% similar expression patterns to Tbp were identified (22,729 total transcripts). (C) Venn diagram illustrating the comparative gene expression analysis of both data sets described above. In total, 17 probe sets belonged to both data sets and represent transcripts whose expression increased during prion disease but did not change with cuprizone treatment. This overlap signifies transcripts whose expression is related to prion disease and not associated to the changing cell population that occurs during neuroinflammation.