Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1984 Aug;20(2):159–161. doi: 10.1128/jcm.20.2.159-161.1984

Comparison of fluorescence polarization immunoassay and bioassay of vancomycin.

D J Pohlod, L D Saravolatz, M M Somerville
PMCID: PMC271276  PMID: 6436291

Abstract

Human serum samples were analyzed for vancomycin concentrations by two different methods: the fluorescence polarization immunoassay and the disk plate bioassay. Each assay method offered acceptable precision. The correlation between both assay methods was excellent (correlation coefficient = 0.985). Excluding technical time, the bioassay was the least expensive method to perform but was more labor intensive than the fluorescence polarization immunoassay.

Full text

PDF
159

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barcenas C. G., Fuller T. J., Elms J., Cohen R., White M. G. Staphylococcal sepsis in patients on chronic hemodialysis regimens: intravenous treatment with vancomycin given once weekly. Arch Intern Med. 1976 Oct;136(10):1131–1134. [PubMed] [Google Scholar]
  2. Barrett F. F., McGehee R. F., Jr, Finland M. Methicillin-resistant Staphylococcus aureus at Boston City Hospital. Bacteriologic and epidemiologic observations. N Engl J Med. 1968 Aug 29;279(9):441–448. doi: 10.1056/NEJM196808292790901. [DOI] [PubMed] [Google Scholar]
  3. Cook F. V., Farrar W. E., Jr Vancomycin revisited. Ann Intern Med. 1978 Jun;88(6):813–818. doi: 10.7326/0003-4819-88-6-813. [DOI] [PubMed] [Google Scholar]
  4. Craven D. E., Reed C., Kollisch N., DeMaria A., Lichtenberg D., Shen K., McCabe W. R. A large outbreak of infections caused by a strain of Staphylococcus aureus resistant of oxacillin and aminoglycosides. Am J Med. 1981 Jul;71(1):53–58. doi: 10.1016/0002-9343(81)90258-8. [DOI] [PubMed] [Google Scholar]
  5. Eykyn S., Phillips I., Evans J. Vancomycin for staphylococcal shunt site infections in patients on regular haemodialysis. Br Med J. 1970 Jul 11;3(5714):80–82. doi: 10.1136/bmj.3.5714.80. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Farber B. F., Moellering R. C., Jr Retrospective study of the toxicity of preparations of vancomycin from 1974 to 1981. Antimicrob Agents Chemother. 1983 Jan;23(1):138–141. doi: 10.1128/aac.23.1.138. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fekety R. Vancomycin. Med Clin North Am. 1982 Jan;66(1):175–181. doi: 10.1016/s0025-7125(16)31451-1. [DOI] [PubMed] [Google Scholar]
  8. Filburn B. H., Shull V. H., Tempera Y. M., Dick J. D. Evaluation of an automated fluorescence polarization immunoassay for vancomycin. Antimicrob Agents Chemother. 1983 Aug;24(2):216–220. doi: 10.1128/aac.24.2.216. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fong K. L., Ho D. H., Bogerd L., Pan T., Brown N. S., Gentry L., Bodey G. P., Sr Sensitive radioimmunoassay for vancomycin. Antimicrob Agents Chemother. 1981 Jan;19(1):139–143. doi: 10.1128/aac.19.1.139. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Geraci J. E. Vancomycin. Mayo Clin Proc. 1977 Oct;52(10):631–634. [PubMed] [Google Scholar]
  11. Jolley M. E., Stroupe S. D., Schwenzer K. S., Wang C. J., Lu-Steffes M., Hill H. D., Popelka S. R., Holen J. T., Kelso D. M. Fluorescence polarization immunoassay. iii. an automated system for therapeutic drug determination. Clin Chem. 1981 Sep;27(9):1575–1579. [PubMed] [Google Scholar]
  12. Karchmer A. W., Dismukes W. E., Buckley M. J., Austen W. G. Late prosthetic valve endocarditis: clinical features influencing therapy. Am J Med. 1978 Feb;64(2):199–206. doi: 10.1016/0002-9343(78)90046-3. [DOI] [PubMed] [Google Scholar]
  13. Magera B. E., Arroyo J. C., Rosansky S. J., Postic B. Vancomycin pharmacokinetics in patients with peritonitis on peritoneal dialysis. Antimicrob Agents Chemother. 1983 May;23(5):710–714. doi: 10.1128/aac.23.5.710. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. McClain J. B., Bongiovanni R., Brown S. Vancomycin quantitation by high-performance liquid chromatography in human serum. J Chromatogr. 1982 Sep 10;231(2):463–466. doi: 10.1016/s0378-4347(00)81873-3. [DOI] [PubMed] [Google Scholar]
  15. Morris A. J., Bilinsky R. T. Prevention of staphylococcal shunt infections by continuous vancomycin prophylaxis. Am J Med Sci. 1971 Aug;262(2):87–92. doi: 10.1097/00000441-197108000-00003. [DOI] [PubMed] [Google Scholar]
  16. Sabath L. D., Casey J. I., Ruch P. A., Stumpf L. L., Finland M. Rapid microassay of gentamicin, kanamycin, neomycin, streptomycin, and vancomycin in serum or plasma. J Lab Clin Med. 1971 Sep;78(3):457–463. [PubMed] [Google Scholar]
  17. Saravolatz L. D., Pohlod D. J., Arking L. M. Community-acquired methicillin-resistant Staphylococcus aureus infections: a new source for nosocomial outbreaks. Ann Intern Med. 1982 Sep;97(3):325–329. doi: 10.7326/0003-4819-97-3-325. [DOI] [PubMed] [Google Scholar]
  18. Stevens P., Young L. S. Simple method for elimination of aminoglycosides from serum to permit bioassay of other antimicrobial agents. Antimicrob Agents Chemother. 1977 Aug;12(2):286–287. doi: 10.1128/aac.12.2.286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Walker C. A., Kopp B. Sensitive bioassay for vancomycin. Antimicrob Agents Chemother. 1978 Jan;13(1):30–33. doi: 10.1128/aac.13.1.30. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Walker C. N. Bioassay for determination of vancomycin in the presence of rifampin or aminoglycosides. Antimicrob Agents Chemother. 1980 Apr;17(4):730–731. doi: 10.1128/aac.17.4.730. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES