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. 2008 Nov 12;28(46):11746–11752. doi: 10.1523/JNEUROSCI.3860-08.2008

Modes and Mishaps of Neuronal Migration in the Mammalian Brain

Christine Métin 1, Richard B Vallee 2, Pasko Rakic 3, Pradeep G Bhide 4,
PMCID: PMC2713018  NIHMSID: NIHMS116055  PMID: 19005035

Abstract

The ability of neurons to migrate to their appropriate positions in the developing brain is critical to brain architecture and function. Recent research has elucidated different modes of neuronal migration and the involvement of a host of signaling factors in orchestrating the migration, as well as vulnerabilities of this process to environmental and genetic factors. Here we discuss the role of cytoskeleton, motor proteins, and mechanisms of nuclear translocation in radial and tangential migration of neurons. We will also discuss how these and other events essential for normal migration of neurons can be disrupted by genetic and environmental factors that contribute to neurological disease in humans.

Introduction

Cell migration is a critical event in the development of the nervous system. Neurons and precursor cells migrate long distances along the dorsal-ventral and anterior–posterior axes of the nervous system during the early embryonic period. In higher vertebrates, including primates, a radial migratory pathway for dispersion of postmitotic neurons has evolved. Rakic (1972) identified radial glial cells as guides for radial neuronal migration in the primate cerebral cortex and put forward the radial unit hypothesis. This hypothesis proposed that clonally related cells migrated from the neuroepithelium toward the cortical surface along the same radial glial fascicle, producing a radial column of cells related by birth (Rakic, 1988).

Later studies showed that some clonally related cells dispersed widely, rather than remaining associated with a single radial glial fascicle (Walsh and Cepko, 1992), and that postmitotic neurons as well as precursor cells migrated tangentially across radial glial fascicles in the developing cerebral cortex (Fishell et al., 1993; O'Rourke et al., 1997). More recently, it was discovered that virtually all interneurons of the cerebral cortex, ∼20–35% of all cortical neurons in rodents, are produced in the ganglionic eminence of the basal forebrain and arrive in the cortex by a ventral-to-dorsal, tangential migratory pathway independent of radial glial guides (de Carlos et al., 1996; Anderson et al., 1997; Tamamaki et al., 1997). In primates, including humans, although many interneurons are produced in the enlarged subventricular zone of the cerebral cortex, the majority migrate from the ganglionic eminence (Letinic et al., 2002; Petanjek et al., 2008a,b). These observations have led to current concepts of cell migration in the developing vertebrate nervous system that include both radial and tangential migration of postmitotic neurons.

A number of genes are known to regulate radial and tangential neuronal migration. The products of these genes mediate a wide range of cellular functions, including chemoattraction/repulsion, cell adhesion, cell motility, and cytoskeletal dynamics. Human neurological disorders, such as lissencephaly, cortical heterotopias, and microcephaly, that are associated with severe cognitive disabilities are attributed to genetic mutations. Accumulating evidence indicates that the products of the genes associated with these disorders mediate cellular functions critical for neuronal migration.

Much of our knowledge about the cellular functions of genes associated with neuronal migration disorders comes from the use of mutant mice and other animal models. For example, a key insight into the genetic regulation of radial neuronal migration came from the mutant mouse reeler, named for its awkward motor coordination. Cortical layers are “upside down” in the reeler mouse because of anomalous neuronal migration (Caviness and Rakic, 1978; Rakic and Caviness, 1995). Subsequent studies revealed that reelin, the product of the reeler gene, is an extracellular matrix molecule essential for radial neuron migration (Rice and Curran, 2001). Critical insights into tangential neuronal migration also came from an animal model: the homeobox gene Dlx1 and Dlx2 double-mutant mouse (Anderson et al., 1997). Thus, although animal models continue to make significant contributions to our understanding of the mechanisms of neuronal migration, and although the basic principles of cortical development are similar across mammalian species, there are important differences between the different species in the timing and sequence of developmental events as well as in the genetic, molecular, and cellular modifications reflecting evolutionary adaptations of considerable functional significance (Bystron et al., 2008).

Apart from genetic factors, environmental factors such as ionizing radiation, neurotransmitter imbalance, and neurotoxins can also impair neuronal migration. The environmental factors may have a particularly significant impact in the large gyrencephalic brains of higher vertebrates that have long curvilinear migratory pathways (Rakic, 2007).

In this symposium, we discuss the role of the cytoskeleton, motor proteins, and mechanisms of nuclear translocation in radial and tangential migration of neurons. We will also discuss how these and other events can be disrupted by genetic and environmental factors that contribute to neurological disease in humans.

Centrosome positioning and microtubule organization in migrating neurons

It is now becoming clear that radial neuron migration in the cerebral cortex is associated with deployment of excitatory “projection” neurons produced in the cortical neuroepithelium, whereas cortical interneurons, produced in the medial ganglionic eminence (MGE) of the basal telencephalon, migrate to the cortex along tangential migratory pathway. Unlike radially migrating neurons, which appear to associate principally with radial glia, the MGE cells can change their cellular partners and direction of migration (Marín and Rubenstein, 2001). Accordingly, migrating MGE cells produce multiple branches as if to sample cellular partners, and select one branch to pursue a partner for migration. Because cell migration is a cyclic process involving stop and go motions, a cyclic pattern in the complex morphological transformations exhibited by migrating MGE cells seemed plausible. When MGE cells migrate on a flat permissive substrate, they reproduce the same migratory behavior as that seen in organotypic slices. Image analysis of the migrating MGE cells showed that phases of branch outgrowth alternated with phases of branch selection. These alternating phases were correlated with nuclear movements (Bellion et al., 2005; Métin et al., 2006).

Nuclear movement of postmitotic MGE cells is a saltatory, two-step process alternating between resting and dynamic phases (Ang et al., 2003; Solecki et al., 2004; Tsai and Gleeson, 2005). During the nuclear resting phase, the centrosome moves forward, ahead of the nucleus. In MGE cells, the centrosome migrates a remarkably long distance away from the nucleus (up to 30 μm), and at the end of its forward migration, the centrosome and the Golgi apparatus fold into a single complex localized to a swelling distinct from the nuclear compartment. This rostral swelling containing the centrosome and Golgi apparatus remains connected to the larger nuclear compartment located at the rear of the cell by an extremely thin link, ∼10–15 μm in length (Fig. 1A,B). Moreover, this phase of spatial dissociation between the nucleus and the centrosome/Golgi complex most often corresponds to a phase of neuritic arbor remodeling.

Figure 1.

Figure 1.

A, In tangentially migrating cells derived from the medial ganglionic eminence (MGE) of the basal forebrain, a centrosome (red) is located at some distance from the resting nucleus (gray). As the cell initiates migration (boxed area), the centrosome (along with Golgi apparatus, not shown) stabilizes in a rostral swelling. Nuclear movement toward the rostral swelling requires actomyosin activity (green). [This panel was modified with permission from Bellion et al. (2005), their Fig. 6.] B, A scanning electron micrograph of a migrating MGE cell showing nuclear (N)–centrosomal (c) dissociation (courtesy of J. P. Baudoin and C. Métin, Institut du Fer à Moulin, Inserm Unité Mixte de Recherche-S 839, Paris, France, used with permission). C, Classical model of centrosomal and nuclear movements in a radially migrating neuron. The centrosome (red) controls the formation of a microtubule network (purple) that surrounds the nucleus, the so-called perinuclear cage, establishing a physical link between centrioles (red) and nuclear membrane. During migration, the forward movement of centrioles leads to deformation of the perinuclear cage, which together with microtubule-associated motor complex (blue) activity pull the nucleus forward.

Morphological transformations of migrating MGE cells reflect evolutionarily conserved functions of microtubule cytoskeleton. In animal cells, the centrosome is a microtubule organizing center that controls microtubule polymerization, and organizes the cytoskeleton by either releasing or anchoring microtubules (Bornens, 2002). In radially migrating neurons, the centrosome controls the formation of a microtubule network that surrounds the nucleus, the so-called perinuclear cage, and establishes a physical link between centrioles and nuclear membrane (Rakic, 1971; Gregory et al., 1988; Rivas and Hatten, 1995; Rakic et al., 1996; Solecki et al., 2004; Higginbotham and Gleeson, 2007; Tsai et al., 2007). During radial migration, the forward movement of centrioles leads to deformation of the perinuclear cage. Cage elasticity together with microtubule-associated motor complex activity cooperate in pulling the nucleus forward (Fig. 1C). This model fits well with the morphological transformations of radially migrating cortical neurons and may explain abnormal nucleokinesis in cortical and cerebellar neurons under pathological conditions (Tanaka et al., 2004). The pronounced dissociation between the nucleus and centrosome (Fig. 1A,B) makes perinuclear cage deformation very unlikely in tangentially migrating cortical interneurons. A more complex organization of microtubules involving dynamic and stable components, neither of which directly converges on the centrosome (Rakic et al., 1996; Umeshima et al., 2007), could exist in MGE cells. These observations raise the possibility that dynamics of additional cytoskeletal components may be able to generate tension forces that might also contribute to nuclear movements in MGE cells. Using blebbistatin, a specific inhibitor of nonmuscle myosin II (Straight et al., 2003), it was shown that actomyosin filaments that accumulate in the perinuclear compartment before nuclear movements play a role in controlling forward nuclear movement (Bellion et al., 2005). A similar mechanism has been described in cells of the subventricular zone that migrate tangentially to the olfactory bulb in the postnatal brain (Schaar and McConnell, 2005) and, more recently, in radially migrating cortical neurons (Tsai et al., 2007). Together these results suggest that actomyosin cytoskeleton might control nuclear movements in neurons exhibiting multiple (radial and tangential) modes of migration.

What is the role of microtubules in tangentially migrating cortical interneurons? This was addressed by examining the effect of nocodazole-induced microtubule destabilization on migration of MGE cells (Baudoin et al., 2008). Low doses of nocodazole impaired leading process morphology and cell directionality but did not affect migration speed. These results suggest a role of microtubules in the control of cell shape rather than cell motility. These findings are in agreement with observations in MGE cells from doublecortin mutant mice that show unchanged migration speed but increased branching of the neuritic arbor (Kappeler et al., 2006). Whether tangentially migrating neurons differ from radially migrating neurons with respect to cytoskeletal organization or whether tangentially and radially migrating neurons share fundamental structural and functional properties remains unclear (Higginbotham and Gleeson, 2007). Solving this question will require a more detailed picture of the organization of microtubules in tangentially migrating neurons at different phases of the migration cycle.

Cellular and molecular causes of impaired migration in lissencephaly

Lissencephaly, or “smooth brain,” is a developmental disease that affects neocortical neuronal migration. Type I, or classical lissencephaly, results from sporadic mutations in the LIS1 gene, resulting in mispositioning of neurons in the neocortex and severe loss of gyri. LIS1 interacts genetically with the motor protein cytoplasmic dynein (Xiang et al., 1995). LIS1 also colocalizes with dynein at diverse subcellular sites, and common phenotypic effects are observed for LIS1 and dynein genes in neuronal and non-neuronal cells (Faulkner et al., 2000; Smith et al., 2000; Shu et al., 2004; Tanaka et al., 2004; Grabham et al., 2007). These results suggest a role for LIS1 in cytoplasmic dynein regulation and, conversely, a potential role for cytoplasmic dynein in neuronal migration and brain development.

Vector-based RNA interference (RNAi) performed using in utero electroporation in embryonic rat brain revealed LIS1 to have multiple, discrete roles in the neuronal migration pathway (Tsai et al., 2005). LIS1 RNAi blocked interkinetic nuclear migration within radial glial progenitor cells. The nuclei were arrested at random distances from the ventricular surface, and mitotic divisions were severely reduced. The latter analysis suggested that nuclei may have an absolute requirement to reach the ventricular surface to enter mitosis (Vallee and Tsai, 2006). Most neuronal precursors generated from the radial glia ascend to the subventricular zone (SVZ), where they convert to a nonmotile multipolar form. After a substantial pause in this state, the cells convert to a bipolar form and migrate outward along radial glial fibers. Conversion to the migratory form was largely blocked by LIS1 RNAi (Tsai et al., 2005), resulting in a substantial accumulation of multipolar cells in the SVZ. Finally, those few cells that did progress to the bipolar state were arrested in this condition, unable to migrate along radial glial fibers. These results together suggested a major role for LIS1 and, presumably, its regulatory target, cytoplasmic dynein, in several aspects of neuronal migration, including glial-guided radial migration.

To understand the cellular basis for these effects, in utero electroporation was used to coexpress LIS1, cytoplasmic dynein, and myosin II short hairpin RNAs along with nuclear, centrosomal, and microtubule markers (Tsai et al., 2007). Again, live analysis of migrating cells in slice culture was used to ensure that cellular and subcellular behavior faithfully reflected in vivo events. In these preparations, the centrosome advanced continuously and much farther ahead of the nucleus than is seen in dissociated neural precursor cell cultures (Fig. 2A). The nucleus, in contrast, advanced in a very discontinuous manner, resulting in a great separation from the centrosome (Tsai et al., 2007). LIS1 and dynein RNAi each blocked centrosomal (Fig. 2A) as well as nuclear movement, whereas myosin II RNAi and the myosin II inhibitor blebbistatin each affected nuclear movement alone. These results identified cytoplasmic dynein as the motor for centrosome movement, and cytoplasmic dynein together with myosin II as the motors for nucleokinesis.

Figure 2.

Figure 2.

A, X–Y tracing of centrosomal movement in radially migrating neural precursor cells monitored in live brain slice culture for up to 3 h (Tsai et al., 2007). Centrosomes (initial position indicated by colored dots) exhibited long-range directed travel, which was inhibited by LIS1 or cytoplasmic dynein heavy chain (HC) RNAi. B, Behavior of the microtubule plus end-tracking protein GFP-EB3 was monitored for 2 min. Traces representing all growing microtubule plus ends within a given video field (arrowheads) are shown for the cell body region, the leading “migratory” process (middle panel), and the trailing axon (seen in right panel). [Tsai et al. (2007), their Fig. 3, modified with permission].

The organization and orientation of cytoskeletal elements have been difficult to define in neuronal precursors. Microtubule plus end-tracking protein GFP-EB3 imaging (Fig. 2B) was used to define the distribution of microtubules in live migrating neuronal precursors in brain slices (Tsai et al., 2007). Microtubule growth occurred at rates of 15–20 μm/min throughout the processes and soma of these cells. Imaging throughout the entire network of growing microtubules in cells at different stages of centrosome-to-nucleus separation provided a comprehensive map of the microtubule cytoskeleton (Fig. 2B). Unlike mature neurons, but as in other blast-like cells, microtubules were seen to emanate from the centrosome and extend radially into both leading and trailing processes. The entire microtubule cytoskeleton advanced with the centrosome at its center, and with the nucleus trailing. Dynein immunocytochemistry revealed marked accumulations of the motor protein in the nuclear region and in transient swellings located with migratory process of locomoting neurons. These results led to a model according to which cytoplasmic dynein regulated by LIS1 pulls on the microtubule network from the swellings (Vallee and Tsai, 2006; Tsai et al., 2007). The nucleus, in a behavior unique to neuronal precursor cells, advances independently by retrograde transport toward the centrosome, also pushed from behind by myosin II.

The role of LIS1 in cytoplasmic dynein regulation is poorly understood. LIS1 binds to several sites within the dynein molecule, including the motor domain (Faulkner et al., 2000; Sasaki et al., 2000; Smith et al., 2000; Tai et al., 2002). LIS1 and dynein also bind NudE and NudEL (Feng et al., 2000; Niethammer et al., 2000; Sasaki et al., 2000; Stehman et al., 2007), which also participate in the neuronal migration pathway (Feng and Walsh, 2004; Shu et al., 2004; Sasaki et al., 2005). The role of LIS1, NudE, and NudEL in dynein motor regulation is not known, although NudE and NudEL bind to subunits within the base of the dynein molecule (Stehman et al., 2007) and may serve in recruiting dynein to diverse subcellular cargoes (Guo et al., 2006; Stehman et al., 2007; Vergnolle and Taylor, 2007).

Dopamine, cocaine, torsinA, and tangential migration of GABA neurons

The role of neurotransmitters in the regulation of neurogenesis and neuronal migration is becoming increasingly evident (Komuro and Rakic, 1992; Nguyen et al., 2001; Owens and Kriegstein, 2002). As a result, insights into the consequences of impaired neurotransmitter signaling for neuronal migration in the embryonic brain are beginning to emerge. Dopamine is a neurotransmitter that can influence neurogenesis and tangential migration of cortical GABA neurons (Ohtani et al., 2003; Popolo et al., 2004; Crandall et al., 2007; McCarthy et al., 2007). Activation of the dopamine D1-like receptor promotes, whereas that of the D2-like receptor decreases, GABA neuron migration from the basal forebrain to the cerebral cortex (Crandall et al., 2007). Dopamine receptor activation results in redistribution of cytoskeletal elements in cultured basal forebrain neurons (Crandall et al., 2007), suggesting a molecular basis for the effects of dopamine on neuronal migration. Specifically, activation of the D1-like receptors mobilizes cytoplasmic dynein heavy chain and tubulin to cellular processes, whereas activation of the D2 receptors produces a condensation of these proteins around the nucleus (Crandall et al., 2007).

Dopamine is also a target of the psychomotor stimulant drug cocaine. Cocaine targets the dopaminergic system by binding to the dopamine transporter, decreasing dopamine reuptake and increasing extracellular dopamine levels (Ritz et al., 1987; Meyer et al., 1993). Persisting increases in extracellular dopamine result in impairment of dopamine receptor function. A number of studies confirm that fetal cocaine exposure attenuates dopamine D1 receptor signaling by uncoupling the receptor from its G-protein partner (Wang et al., 1995). This is achieved by inhibiting protein phosphatase-1 and shifting the balance in favor of the uncoupled state of the D1 receptor (Zhen et al., 2001).

Because cocaine exposure disrupts dopamine D1 receptor signaling and because D1 receptor activation promotes GABA neuron migration to the cerebral cortex, one can predict that exposure of the developing fetus to cocaine would decrease GABA neuron migration to the cortex. In fact, in slice preparations of brains from embryonic mice exposed to cocaine in utero, fewer cells migrated from the ganglionic eminence to the cerebral cortex (Crandall et al., 2004). Moreover, there was a significant decrease in the numbers of GABA-immunoreactive cells in the intact cerebral cortex of embryonic mice exposed to cocaine in utero (Crandall et al., 2004). These observations suggest that cocaine-induced perturbations in dopamine receptor signaling may impact migration of GABA neurons to the cerebral cortex.

The effects of in utero cocaine exposure are not limited to GABA neuron migration. It also disrupts the organization of radial glial fascicles in the embryonic mouse neocortex, leading to persistence of radial glial fascicles and an overall decrease in glial profiles (Gressens et al., 1992). Both primate and rodent models of in utero cocaine exposure report significant alterations in the histology of the embryonic and mature cerebral cortex. In the primate model, in utero cocaine exposure produced loss of cortical lamination at 2 months of age to such an extent that only the cell-poor layer I could be readily distinguished in conventional histological sections (Lidow et al., 2001; He and Lidow, 2004). Thus, the possibility exists that in utero cocaine exposure can impair radial neuronal migration also.

Dopamine receptor signaling mechanisms are impaired in movement disorders such as dystonia. Early-onset generalized torsion dystonia is a dominantly inherited childhood-onset movement disorder caused in most cases by a 3 bp GAG deletion in the DYT1 gene (Ozelius et al., 1997). TorsinA is the product of the DYT1 gene, and recent evidence points to an unexpected role for torsinA in the regulation of GABA neuron migration from the basal forebrain to the cerebral cortex. The majority of torsinA is localized within the nuclear envelope and the contiguous endoplasmic reticulum (Hewett et al., 2000; Kustedjo et al., 2000; Liu et al., 2003; Bragg et al., 2004), where it is thought to act as an intermediary for linking the nuclear envelope to cytoskeletal proteins via its association with SUN and nesprin proteins. Specifically, torsinA interacts with the KASH domain of nesprins (Nery et al., 2008), which span the outer nuclear membrane and link the latter to intermediate filaments in the cytoplasm (Wilhelmsen et al., 2005). In fibroblasts from torsinA knock-out mice or DYT1 dystonia patients, nesprin-3 changes its distribution to become more predominant in the endoplasmic reticulum (Nery et al., 2008). Perhaps more interestingly, fibroblasts from torsinA knock-out mice show significant impairment of nuclear polarization and cell migration in a wound-healing assay (Nery et al., 2008), suggesting a role of torsinA in nuclear translocation in migrating cells.

Because torsinA is expressed in the embryonic basal forebrain and cerebral cortex (Siegert et al., 2005; Vasudevan et al., 2006), and because it could play a role in tethering the nucleus to the cytoskeleton, it is reasonable to suggest that it plays a role in neuronal migration. In fact, recent evidence indicates significant decreases in migration of MGE cells in explant cultures obtained from torsinA knock-out mice (Bhide et al., 2008). Therefore, it appears that torsinA may influence GABA neuron migration in the embryonic brain by virtue of its dynamic interactions with nuclear envelope proteins that link the nucleus to the cytoskeleton, a key link in the mechanics of neuronal migration.

Mechanisms of neuronal migration and disorders of neuronal position: a synopsis

In the preceding sections we reviewed cellular and molecular mechanisms of neuronal migration that involve reorganization of the cytoskeleton and movement of the nucleus. We also discussed the role of motor proteins, nuclear envelope components, and neurotransmitter receptors in the regulation of the neuronal migration. A host of genetic factors are involved in the regulation of specific components of the neuronal migration process, such as progenitor proliferation, cell cycle exit, establishment of polarity, detachment from the local substrate, and rate of nuclear and somal translocation (for review, see Rakic, 2007). As a result, the precise radial and tangential deployment of cerebral cortical neurons is highly vulnerable to a large number of genetic mutations as well as various physical (e.g., ionizing radiation, ultrasonic waves, and heat), chemical (e.g., drugs and alcohol), and biological (e.g., neurotrophic viruses) agents. Disruption or even slowing down of neuronal migration may result not only in gross and obvious but also subtle abnormalities in neuronal positioning that indirectly affect the pattern of synaptic circuits (Algan and Rakic, 1997; Ang et al., 2006).

Severe malformations such as microencephaly, schizencephaly, lissencephaly, macrogyria, or polymicrogyria are to a major extent a consequence of defective migration and abnormal settling of nerve cells. Other migratory abnormalities can be revealed in the mature brain by the presence of groups or solitary ectopic neurons that have failed to reach their proper targets and are scattered within the white matter. However, in many cases the neuronal malpositioning may be subtle and cannot be detected readily by conventional histopathologic techniques. Such misplaced neurons may be involved in a variety of idiopathic neurological disorders that range from childhood epilepsy and mental retardation to autism and developmental dyslexia. Because the detection of improper position of isochronously generated neurons requires DNA labeling and/or use of transgenic animal models, not applicable to human, the subtle migratory abnormalities could be entirely missed by routine imaging of live or autopsied brains.

In conclusion, the identity, synaptic relationship and, ultimately, function of neurons in the brain depends on their proper location, which is achieved by long distance migration in the embryonic brain. In the cerebral cortex, neurons reach their final destinations via radial or tangential migratory pathways. Based on a plethora of evidence for the involvement of multiple molecules in regulation of complex cellular events essential for neuronal placement, it is now possible to propose working models of the sequence of gene expression, cascades of multiple molecular pathways, and complex cell-cell interactions that are involved in normal as well as abnormal neuronal migration (Rakic, 2007). These models show how multiple causes can produce similar cognitive disorders and how the same genetic or environmental factor can produce diverse malformations, depending on when and where the causative factors act. Thus, a new challenge is to develop methods for detection of subtle laminar and radial misplacement of neurons to determine how this deviation affects the highest cognitive functions.

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