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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1984 Sep;20(3):347–350. doi: 10.1128/jcm.20.3.347-350.1984

Radioimmunofocus assay for detection and quantitation of human rotavirus.

S Liu, C Birch, A Coulepis, I Gust
PMCID: PMC271327  PMID: 6092422

Abstract

A radioimmunofocus assay suitable for quantitation of cell culture-adapted human rotavirus was developed. The method was reproducible, more sensitive than plaque assay, and useful to detect and quantify strains of rotavirus which do not produce plaques. Preliminary results also suggested that the technique will be a useful means of serotyping cell culture-adapted strains of the virus.

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Selected References

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  1. Birch C. J., Rodger S. M., Marshall J. A., Gust I. D. Replication of human rotavirus in cell culture. J Med Virol. 1983;11(3):241–250. doi: 10.1002/jmv.1890110307. [DOI] [PubMed] [Google Scholar]
  2. Greenberg H. B., Wyatt R. G., Kapikian A. Z., Kalica A. R., Flores J., Jones R. Rescue and serotypic characterization of noncultivable human rotavirus by gene reassortment. Infect Immun. 1982 Jul;37(1):104–109. doi: 10.1128/iai.37.1.104-109.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hasegawa A., Matsuno S., Inouye S., Kono R., Tsurukubo Y., Mukoyama A., Saito Y. Isolation of human rotaviruses in primary cultures of monkey kidney cells. J Clin Microbiol. 1982 Aug;16(2):387–390. doi: 10.1128/jcm.16.2.387-390.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kutsuzawa T., Konno T., Suzuki H., Kapikian A. Z., Ebina T., Ishida N. Isolation of human rotavirus subgroups 1 and 2 in cell culture. J Clin Microbiol. 1982 Oct;16(4):727–730. doi: 10.1128/jcm.16.4.727-730.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Lemon S. M., Binn L. N., Marchwicki R. H. Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures. J Clin Microbiol. 1983 May;17(5):834–839. doi: 10.1128/jcm.17.5.834-839.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Salacinski P. R., McLean C., Sykes J. E., Clement-Jones V. V., Lowry P. J. Iodination of proteins, glycoproteins, and peptides using a solid-phase oxidizing agent, 1,3,4,6-tetrachloro-3 alpha,6 alpha-diphenyl glycoluril (Iodogen). Anal Biochem. 1981 Oct;117(1):136–146. doi: 10.1016/0003-2697(81)90703-x. [DOI] [PubMed] [Google Scholar]
  7. Sato K., Inaba Y., Shinozaki T., Fujii R., Matumoto M. Isolation of human rotavirus in cell cultures: brief report. Arch Virol. 1981;69(2):155–160. doi: 10.1007/BF01315159. [DOI] [PubMed] [Google Scholar]
  8. Urasawa S., Urasawa T., Taniguchi K. Three human rotavirus serotypes demonstrated by plaque neutralization of isolated strains. Infect Immun. 1982 Nov;38(2):781–784. doi: 10.1128/iai.38.2.781-784.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Urasawa T., Urasawa S., Taniguchi K. Sequential passages of human rotavirus in MA-104 cells. Microbiol Immunol. 1981;25(10):1025–1035. doi: 10.1111/j.1348-0421.1981.tb00109.x. [DOI] [PubMed] [Google Scholar]
  10. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982 Jul;37(1):110–115. doi: 10.1128/iai.37.1.110-115.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wyatt R. G., James H. D., Jr, Pittman A. L., Hoshino Y., Greenberg H. B., Kalica A. R., Flores J., Kapikian A. Z. Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. J Clin Microbiol. 1983 Aug;18(2):310–317. doi: 10.1128/jcm.18.2.310-317.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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