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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1984 Sep;20(3):357–361. doi: 10.1128/jcm.20.3.357-361.1984

Comparison of enzyme-linked immunosorbent and indirect hemagglutination assays for determining anthrax antibodies.

A Johnson-Winegar
PMCID: PMC271329  PMID: 6436303

Abstract

An enzyme-linked immunosorbent assay has been established to measure anthrax antibody titers. The protective antigen component of anthrax toxin was used as the capture antigen. Two types of conjugates (protein A-horseradish peroxidase and anti-human immunoglobulin G plus immunoglobulin A plus immunoglobulin M-horseradish peroxidase) were tested. Results from enzyme-linked immunosorbent assay testing were compared with those from indirect hemagglutination titers on serum from vaccinees. The overall trend of enzyme-linked immunosorbent assay and indirect hemagglutination titers was significant. The enzyme-linked immunosorbent assay offered speed, precision, and reduced cost per test.

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Selected References

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