Abstract
Canine fecal samples were analyzed by enzyme-linked immunosorbent assays (ELISA) by using monoclonal antibodies to the canine parvovirus hemagglutinating protein. These data were compared with results obtained with DNA hybridization assays, hemagglutination assays, and electron microscopy. The highest correlation was observed between the ELISA and the hemagglutination tests, with 94.4% of samples showing agreement. Lower correlation was obtained between ELISA and DNA hybridization tests (73.3%). Correlation between ELISA and electron microscopy was 60.9%. The studies indicated that the ELISA can be used as a sensitive and specific diagnostic assay for canine parvovirus infections.
Full text
PDF





Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Appel M. J., Scott F. W., Carmichael L. E. Isolation and immunisation studies of a canine parco-like virus from dogs with haemorrhagic enteritis. Vet Rec. 1979 Aug 25;105(8):156–159. doi: 10.1136/vr.105.8.156. [DOI] [PubMed] [Google Scholar]
- Carman P. S., Povey R. C. Comparison of the viral proteins of canine parvovirus-2, mink enteritis virus and feline panleukopenia virus. Vet Microbiol. 1983 Oct;8(5):423–435. doi: 10.1016/0378-1135(83)90037-8. [DOI] [PubMed] [Google Scholar]
- Carmichael L. E., Binn L. N. New enteric viruses in the dog. Adv Vet Sci Comp Med. 1981;25:1–37. [PubMed] [Google Scholar]
- Carmichael L. E., Joubert J. C., Pollock R. V. Hemagglutination by canine parvovirus: serologic studies and diagnostic applications. Am J Vet Res. 1980 May;41(5):784–791. [PubMed] [Google Scholar]
- Gagnon A. N., Povey R. C. A possible parvovirus associated with an epidemic gastroenteritis of dogs in Canada. Vet Rec. 1979 Mar 24;104(12):263–264. doi: 10.1136/vr.104.12.263. [DOI] [PubMed] [Google Scholar]
- Gordon J. C., Rogers W. A. Field evaluation of a canine parvovirus vaccination program, using feline origin modified live virus vaccine. J Am Vet Med Assoc. 1982 Jun 15;180(12):1429–1431. [PubMed] [Google Scholar]
- Kramer J. M., Meunier P. C., Pollock R. V. Canine parvovirus: update. Vet Med Small Anim Clin. 1980 Oct;75(10):1541–1555. [PubMed] [Google Scholar]
- Lutz H., Pedersen N. C., Durbin R., Theilen G. H. Monoclonal antibodies to three epitopic regions of feline leukemia virus p27 and their use in enzyme-linked immunosorbent assay of p27. J Immunol Methods. 1983 Jan 28;56(2):209–220. doi: 10.1016/0022-1759(83)90413-1. [DOI] [PubMed] [Google Scholar]
- Mathys A., Mueller R., Pedersen N. C., Theilen G. H. Comparison of hemagglutination and competitive enzyme-linked immunosorbent assay procedures for detecting canine parvovirus in feces. Am J Vet Res. 1983 Jan;44(1):152–154. [PubMed] [Google Scholar]
- Mathys A., Mueller R., Pedersen N. C., Theilen G. H. Hemagglutination with formalin-fixed erythrocytes for detection of canine parvovirus. Am J Vet Res. 1983 Jan;44(1):150–151. [PubMed] [Google Scholar]
- Parrish C. R., Carmichael L. E. Antigenic structure and variation of canine parvovirus type-2, feline panleukopenia virus, and mink enteritis virus. Virology. 1983 Sep;129(2):401–414. doi: 10.1016/0042-6822(83)90179-4. [DOI] [PubMed] [Google Scholar]
- Rice J. B., Winters K. A., Krakowka S., Olsen R. G. Comparison of systemic and local immunity in dogs with canine parvovirus gastroenteritis. Infect Immun. 1982 Dec;38(3):1003–1009. doi: 10.1128/iai.38.3.1003-1009.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yolken R. H. Use of monoclonal antibodies for viral diagnosis. Curr Top Microbiol Immunol. 1983;104:177–195. doi: 10.1007/978-3-642-68949-9_11. [DOI] [PubMed] [Google Scholar]