Abstract
An improved bioluminescent assay of gentamicin in serum, based on the dose-dependent effect of the agent on the accumulation of extracellular ATP in Escherichia coli LU 14 cultures, is presented. The accuracy of the bioluminescent assay of gentamicin, expressed as the mean coefficient of variation over the therapeutic range, was 2.8%. Corresponding figures for an agar diffusion assay, a latex agglutination inhibition card test, an enzyme immunoassay, and a fluorescence immunoassay were 6.4, 17.5, 4.2, and 9.4%, respectively. All of the methods correlated well (r = 0.926 to 0.976), with the possible exception of the card test (r = 0.777 to 0.841). The bioluminescent assay requires only 1 microliter of serum, which allows for capillary sampling, and results are available within 75 min.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Burd J. F., Wong R. C., Feeney J. E., Carrico R. J., Boguslaski R. C. Homogeneous reactant-labeled fluorescent immunoassay for therapeutic drugs exemplified by gentamicin determination in human serum. Clin Chem. 1977 Aug;23(8):1402–1408. [PubMed] [Google Scholar]
- Harber M. J., Asscher A. W. A new method for antibiotic assay based on measurement of bacterial adenosine triphosphate using the firefly bioluminescence system. J Antimicrob Chemother. 1977 Jan;3(1):35–41. doi: 10.1093/jac/3.1.35. [DOI] [PubMed] [Google Scholar]
- Lundin A., Richardsson A., Thore A. Continous monitoring of ATP-converting reactions by purified firefly luciferase. Anal Biochem. 1976 Oct;75(2):611–620. doi: 10.1016/0003-2697(76)90116-0. [DOI] [PubMed] [Google Scholar]
- Nilsson L., Höjer H., Anséhn S., Thore A. A rapid semiautomated bioassay of gentamicin based on luciferase assay of bacterial adenosine triphosphate. Scand J Infect Dis. 1977;9(3):232–236. doi: 10.3109/inf.1977.9.issue-3.15. [DOI] [PubMed] [Google Scholar]
- Nilsson L. New rapid bioassay of gentamicin based on luciferase assay of extracellular ATP in bacterial cultures. Antimicrob Agents Chemother. 1978 Dec;14(6):812–816. doi: 10.1128/aac.14.6.812. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phaneuf D., Francke E., Neu H. C. Rapid, reproducible enzyme immunoassay for gentamicin. J Clin Microbiol. 1980 Mar;11(3):266–269. doi: 10.1128/jcm.11.3.266-269.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Standiford H. C., Bernstein D., Nipper H. C., Caplan E., Tatem B., Hall J. S., Reynolds J. Latex agglutination inhibition card test for gentamicin assay: clinical evaluation and comparison with radioimmunoassay and bioassay. Antimicrob Agents Chemother. 1981 Apr;19(4):620–624. doi: 10.1128/aac.19.4.620. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thore A., Nilsson L., Höjer H., Anséhn S., Bröte L. Effects of ampicillin on intracellular levels of adenosine triphosphate in bacterial cultures related to antibiotic susceptibility. Acta Pathol Microbiol Scand B. 1977 Apr;85(2):161–166. doi: 10.1111/j.1699-0463.1977.tb01690.x. [DOI] [PubMed] [Google Scholar]
- Wagman G. H., Bailey J. V., Weinstein M. J. Binding of aminoglycoside antibiotics to filtration materials. Antimicrob Agents Chemother. 1975 Mar;7(3):316–319. doi: 10.1128/aac.7.3.316. [DOI] [PMC free article] [PubMed] [Google Scholar]