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. 1984 Sep;20(3):405–408. doi: 10.1128/jcm.20.3.405-408.1984

Detection of enterovirus 70 with monoclonal antibodies.

L J Anderson, M H Hatch, M R Flemister, G E Marchetti Jr
PMCID: PMC271339  PMID: 6092426

Abstract

To improve the ability to identify enterovirus-70 (EV-70) from patients with acute hemorrhagic conjunctivitis, we developed four monoclonal antibodies (MAbs) to EV-70. We reacted the four MAbs against nine previously characterized strains of EV-70 and heterologous viruses by virus neutralization, indirect immunofluorescence, and enzyme-linked immunosorbent assay (ELISA). Two of the MAbs neutralized all nine strains of EV-70 and none of the other enterovirus types tested. Two of the MAbs gave a positive reaction with all nine strains by indirect immunofluorescence, and three reacted with all nine strains by ELISA. None of the MAbs gave a positive reaction with heterologous viruses, including those associated with eye disease, by indirect immunofluorescence or ELISA. The two neutralizing MAbs failed to give a positive reaction with some of the strains of EV-70 by indirect immunofluorescence and ELISA, yet they neutralized these viruses. By ELISA with a polyclonal serum as capture antibody and a mixture of MAbs as detector antibody, we were able to detect from 10(2.2) to 10(5.8) 50% tissue culture infective doses of virus and to type lyophilized isolates of EV-70 sent from Taiwan from which we could not recover infectious virus. By choosing the appropriate MAb, or mixture of MAbs, we could construct a test which had the type specificity and strain sensitivity needed to type isolates of EV-70.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bell E. J., Cosgrove B. P. Routine enterovirus diagnosis in a human rhabdomyosarcoma cell line. Bull World Health Organ. 1980;58(3):423–428. [PMC free article] [PubMed] [Google Scholar]
  2. Cepko C. L., Whetstone C. A., Sharp P. A. Adenovirus hexon monoclonal antibody that is group specific and potentially useful as a diagnostic reagent. J Clin Microbiol. 1983 Feb;17(2):360–364. doi: 10.1128/jcm.17.2.360-364.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Hatch M. H., Malison M. D., Palmer E. L. Isolation of enterovirus 70 from patients with acute hemorrhagic conjunctivitis in Key West, Florida. N Engl J Med. 1981 Dec 31;305(27):1648–1648. doi: 10.1056/NEJM198112313052711. [DOI] [PubMed] [Google Scholar]
  4. Hierholzer J. C., Hilliard K. A., Esposito J. J. Serosurvey for "acute hemorrhagic conjunctivitis" virus (Enterovirus 70) antibodies in the Southeastern United States, with review of the literature and some epidemiologic implications. Am J Epidemiol. 1975 Dec;102(6):533–544. doi: 10.1093/oxfordjournals.aje.a112191. [DOI] [PubMed] [Google Scholar]
  5. Kendal A. P., Phillips D. J., Webster R. G., Galland G. G., Reimer C. B. Effect of test system on the ability of monoclonal antibodies to detect antigenic drift in influenza A(H1N1) virus haemagglutinins. J Gen Virol. 1981 Jun;54(Pt 2):253–261. doi: 10.1099/0022-1317-54-2-253. [DOI] [PubMed] [Google Scholar]
  6. Kew O. M., Nottay B. K., Hatch M. H., Hierholzer J. C., Obijeski J. F. Oligonucleotide fingerprint analysis of enterovirus 70 isolates from the 1980 to 1981 pandemic of acute hemorrhagic conjunctivitis: evidence for a close genetic relationship among Asian and American strains. Infect Immun. 1983 Aug;41(2):631–635. doi: 10.1128/iai.41.2.631-635.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kono R., Sasagawa A., Ishii K., Sugiura S., Ochi M. Pandemic of new type of conjunctivitis. Lancet. 1972 Jun 3;1(7762):1191–1194. doi: 10.1016/s0140-6736(72)90921-x. [DOI] [PubMed] [Google Scholar]
  8. Mirkovic R. R., Schmidt N. J., Yin-Murphy M., Melnick J. L. Enterovirus etiology of the 1970 Singapore epidemic of acute conjunctivitis. Intervirology. 1974;4(2):119–127. doi: 10.1159/000149850. [DOI] [PubMed] [Google Scholar]
  9. Nejmi S., Gaudin O. G., Chomel J. J., Baaj A., Sohier R., Bosshard S. Isolation of a virus responsible for an outbreak of acute haemorrhagic conjunctivitis in Morocco. J Hyg (Lond) 1974 Apr;72(2):181–183. doi: 10.1017/s002217240002338x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Osterhaus A. D., van Wezel A. L., Hazendonk T. G., UytdeHaag F. G., van Asten J. A., van Steenis B. Monoclonal antibodies to polioviruses. Comparison of intratypic strain differentiation of poliovirus type 1 using monoclonal antibodies versus cross-absorbed antisera. Intervirology. 1983;20(2-3):129–136. doi: 10.1159/000149381. [DOI] [PubMed] [Google Scholar]
  11. Pal S. R., Szucs G., Melnick J. L., Kaiwar R., Bhardwaj G., Singh R., Gangwar D. N., Choudhury S., Jain I. S. Immunofluorescence test for the epidemiological monitoring of acute haemorrhagic conjunctivitis cases. Bull World Health Organ. 1983;61(3):485–490. [PMC free article] [PubMed] [Google Scholar]
  12. Pal S. R., Szücs G., Melnick J. L. Rapid immunofluorescence diagnosis of acute hemorrhagic conjunctivitis caused by enterovirus 70. Intervirology. 1983;20(1):19–22. doi: 10.1159/000149369. [DOI] [PubMed] [Google Scholar]
  13. Patriarca P. A., Onorato I. M., Sklar V. E., Schonberger L. B., Kaminski R. M., Hatch M. H., Morens D. M., Forster R. K. Acute hemorrhagic conjunctivitis Investigation of a large-scale community outbreak in Dade County, Florida. JAMA. 1983 Mar 11;249(10):1283–1289. doi: 10.1001/jama.249.10.1283. [DOI] [PubMed] [Google Scholar]
  14. Ritchie D. G., Nickerson J. M., Fuller G. M. Two simple programs for the analysis of data from enzyme-linked immunosorbent (ELISA) assays on a programmable desk-top calculator. Anal Biochem. 1981 Jan 15;110(2):281–290. doi: 10.1016/0003-2697(81)90193-7. [DOI] [PubMed] [Google Scholar]

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