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. 1984 Sep;20(3):441–447. doi: 10.1128/jcm.20.3.441-447.1984

Latex immunoassay for rapid detection of rotavirus.

J H Hughes, A V Tuomari, D R Mann, V V Hamparian
PMCID: PMC271347  PMID: 6092427

Abstract

A latex agglutination (LA) test was evaluated for the detection of human rotaviruses in stool specimens. Both antiserum and immunoglobulin G (IgG)-sensitized latex particles were used, with IgG-coated beads being more sensitive for human rotavirus antigen detection. Latex beads sensitized with anti-simian-SA-11 IgG were stable for at least 8 months when stored at 4 degrees C. The sensitivity of the test was compared with that of the Rotazyme (Abbott Laboratories, Diagnostics Div., North Chicago, Ill.) test. The least number of particles detected was 9.0 X 10(5) particles by the LA test versus 4.5 X 10(5) particles by the Rotazyme test. When 10 stool specimens were serially diluted for antigen endpoint determinations, the geometric mean titer by the LA test was 592 versus 1,280 by the Rotazyme test. Forty-three stool samples positive by the Rotazyme test were all positive by the LA test, and no false negative results were detected. Unconfirmed false positive reactions ranged between 8 and 24%. The LA test for rotavirus antigen detection is direct, easy to perform, sensitive, quick, and may have application for use in diagnostic laboratories, emergency rooms, and physician's offices.

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Selected References

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  1. Beards G. M., Bryden A. S. Evaluation of a new enzyme-linked immunosorbent assay test for rotavirus antigen in faeces. J Clin Pathol. 1981 Dec;34(12):1388–1391. doi: 10.1136/jcp.34.12.1388. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Beards G. M., Campbell A. D., Cottrell N. R., Peiris J. S., Rees N., Sanders R. C., Shirley J. A., Wood H. C., Flewett T. H. Enzyme-linked immunosorbent assays based on polyclonal and monoclonal antibodies for rotavirus detection. J Clin Microbiol. 1984 Feb;19(2):248–254. doi: 10.1128/jcm.19.2.248-254.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop R. F., Davidson G. P., Holmes I. H., Ruck B. J. Detection of a new virus by electron microscopy of faecal extracts from children with acute gastroenteritis. Lancet. 1974 Feb 2;1(7849):149–151. doi: 10.1016/s0140-6736(74)92440-4. [DOI] [PubMed] [Google Scholar]
  4. Blacklow N. R., Cukor G. Viral gastroenteritis. N Engl J Med. 1981 Feb 12;304(7):397–406. doi: 10.1056/NEJM198102123040705. [DOI] [PubMed] [Google Scholar]
  5. Bradburne A. F., Almeida J. D., Gardner P. S., Moosai R. B., Nash A. A., Coombs R. R. A solid-phase system (SPACE) for the detection and quantification of rotavirus in faeces. J Gen Virol. 1979 Sep;44(3):615–623. doi: 10.1099/0022-1317-44-3-615. [DOI] [PubMed] [Google Scholar]
  6. Brandt C. D., Kim H. W., Rodriguez W. J., Thomas L., Yolken R. H., Arrobio J. O., Kapikian A. Z., Parrott R. H., Chanock R. M. Comparison of direct electron microscopy, immune electron microscopy, and rotavirus enzyme-linked immunosorbent assay for detection of gastroenteritis viruses in children. J Clin Microbiol. 1981 May;13(5):976–981. doi: 10.1128/jcm.13.5.976-981.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bryden A. S., Davies H. A., Thouless M. E., Flewitt T. H. Diagnosis of rotavirus infection by cell culture. J Med Microbiol. 1977 Feb;10(1):121–125. doi: 10.1099/00222615-10-1-121. [DOI] [PubMed] [Google Scholar]
  8. Chrystie I. L., Totterdell B. M., Banatvala J. E. Asymptomatic endemic rotavirus infections in the newborn. Lancet. 1978 Jun 3;1(8075):1176–1178. doi: 10.1016/S0140-6736(78)90967-4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Clementi M., Pauri P., Bagnarelli P., Carloni G., Calegari L. Diagnosis of human rotavirus infections: comparison of an electrophoretic method, a modified complement fixation test and electron microscopy for rotavirus detection. Arch Virol. 1981;67(4):341–344. doi: 10.1007/BF01314837. [DOI] [PubMed] [Google Scholar]
  10. Flewett T. H., Bryden A. S., Davies H. Letter: Virus particles in gastroenteritis. Lancet. 1973 Dec 29;2(7844):1497–1497. doi: 10.1016/s0140-6736(73)92760-8. [DOI] [PubMed] [Google Scholar]
  11. Grauballe P. C., Jarzabek Z. Comparison of indirect double antibody and double antibody sandwich ELISA techniques with latex agglutination test for the diagnosis of human rotavirus infection. Acta Virol. 1984 Jan;28(1):59–63. [PubMed] [Google Scholar]
  12. Grauballe P. C., Vestergaard B. F., Meyling A., Genner J. Optimized enzyme-linked immunosorbent assay for detection of human and bovine rotavirus in stools: Comparison with electron-microscopy, immunoelectro-osmophoresis, and fluorescent antibody techniques. J Med Virol. 1981;7(1):29–40. doi: 10.1002/jmv.1890070104. [DOI] [PubMed] [Google Scholar]
  13. Haikala O. J., Kokkonen J. O., Leinonen M. K., Nurmi T., Mäntyjärvi R., Sarkkinen H. K. Rapid detection of rotavirus in stool by latex agglutination: comparison with radioimmunoassay and electron microscopy and clinical evaluation of the test. J Med Virol. 1983;11(2):91–97. doi: 10.1002/jmv.1890110202. [DOI] [PubMed] [Google Scholar]
  14. Hammond G. W., Ahluwalia G. S., Klisko B., Hazelton P. R. Human rotavirus detection by counterimmunoelectrophoresis versus enzyme immunoassay and electron microscopy after direct ultracentrifugation. J Clin Microbiol. 1984 Mar;19(3):439–441. doi: 10.1128/jcm.19.3.439-441.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Harris C. C., Yolken R. H., Krokan H., Hsu I. C. Ultrasensitive enzymatic radioimmunoassay: application to detection of cholera toxin and rotavirus. Proc Natl Acad Sci U S A. 1979 Oct;76(10):5336–5339. doi: 10.1073/pnas.76.10.5336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Hughes J. H., Chema S., Lin N., Conant R. M., Hamparian V. V. Acid lability of rhinoviruses: loss of C and D antigenicity after treatment at pH 3.0. J Immunol. 1974 Mar;112(3):919–925. [PubMed] [Google Scholar]
  17. Hughes J. H., Gnau J. M., Hilty M. D., Chema S., Ottolenghi A. C., Hamparian V. V. Picornaviruses: rapid differentiation and identification by immune electronmicroscopy and immunodiffusion. J Med Microbiol. 1977 May;10(2):203–212. doi: 10.1099/00222615-10-2-203. [DOI] [PubMed] [Google Scholar]
  18. Kalica A. R., Purcell R. H., Sereno M. M., Wyatt R. G., Kim H. W., Chanock R. M., Kapikian A. Z. A microtiter solid phase radioimmunoassay for detection of the human reovirus-like agent in stools. J Immunol. 1977 Apr;118(4):1275–1279. [PubMed] [Google Scholar]
  19. Kapikian A. Z., Cline W. L., Mebus C. A., Wyatt R. G., Kalica A. R., James H. D., Jr, VanKirk D., Chanock R. M. New complement-fixation test for the human reovirus-like agent of infantile gastroenteritis. Nebraska calf diarrhea virus used as antigen. Lancet. 1975 May 10;1(7915):1056–1061. doi: 10.1016/s0140-6736(75)91827-9. [DOI] [PubMed] [Google Scholar]
  20. Kapikian A. Z., Kim H. W., Wyatt R. G., Rodriguez W. J., Ross S., Cline W. L., Parrott R. H., Chanock R. M. Reoviruslike agent in stools: association with infantile diarrhea and development of serologic tests. Science. 1974 Sep 20;185(4156):1049–1053. doi: 10.1126/science.185.4156.1049. [DOI] [PubMed] [Google Scholar]
  21. Kjeldsberg E., Mortensson-Egnund K. Comparison of solid-phase immune electron microscopy, direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in faecal samples. J Virol Methods. 1982 Feb;4(1):45–53. doi: 10.1016/0166-0934(82)90053-2. [DOI] [PubMed] [Google Scholar]
  22. Krause P. J., Hyams J. S., Middleton P. J., Herson V. C., Flores J. Unreliability of Rotazyme ELISA test in neonates. J Pediatr. 1983 Aug;103(2):259–262. doi: 10.1016/s0022-3476(83)80361-8. [DOI] [PubMed] [Google Scholar]
  23. Mathan M., Almeida J. D., Cole J. An antigenic subunit present in rotavirus infected faeces. J Gen Virol. 1977 Feb;34(2):325–329. doi: 10.1099/0022-1317-34-2-325. [DOI] [PubMed] [Google Scholar]
  24. Matsuno S., Nagayoshi S. Quantitative estimation of infantile gastroenteritis virus antigens in stools by immune adherence hemagglutination test. J Clin Microbiol. 1978 Mar;7(3):310–311. doi: 10.1128/jcm.7.3.310-311.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Middleton P. J., Holdaway M. D., Petric M., Szymanski M. T., Tam J. S. Solid-phase radioimmunoassay for the detection of rotavirus. Infect Immun. 1977 May;16(2):439–444. doi: 10.1128/iai.16.2.439-444.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Middleton P. J., Petric M., Hewitt C. M., Szymanski M. T., Tam J. S. Counter-immunoelectro-osmophoresis for the detection of infantile gastroenteritis virus (orbi-group) antigen and antibody. J Clin Pathol. 1976 Mar;29(3):191–197. doi: 10.1136/jcp.29.3.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Nakagomi O., Nakagomi A., Suto T., Suzuki H., Kutsuzawa T., Tazawa F., Konno T., Ishida N. Detection of human rotavirus by reversed passive hemagglutination (RPHA) using antibody against a cultivable human rotavirus as compared with electron microscopy (EM) and enzyme-linked immunosorbent assay (ELISA). Microbiol Immunol. 1982;26(8):747–751. doi: 10.1111/j.1348-0421.1982.tb00219.x. [DOI] [PubMed] [Google Scholar]
  28. Nicolaieff A., Obert G., van Regenmortel M. H. Detection of rotavirus by serological trapping on antibody-coated electron microscope grids. J Clin Microbiol. 1980 Jul;12(1):101–104. doi: 10.1128/jcm.12.1.101-104.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Obert G., Gloeckler R., Burckard J., van Regenmortel M. H. Comparison of immunosorbent electron microscopy, enzyme immunoassay and counterimmunoelectrophoresis for detection of human rotavirus in stools. J Virol Methods. 1981 Sep;3(2):99–107. doi: 10.1016/0166-0934(81)90006-9. [DOI] [PubMed] [Google Scholar]
  30. Peterson M. W., Spendlove R. S., Smart R. A. Detection of neonatal calf diarrhea virus, infant reovirus-like diarrhea virus, and a coronavirus using the fluorescent virus precipitin test. J Clin Microbiol. 1976 Mar;3(3):376–377. doi: 10.1128/jcm.3.3.376-377.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Rhodes M. B., Stair E. L., McCullough R. A., McGill L. D., Mebus C. A. Comparison of results using electron microscope, immunodiffusion and fluorescent antibody analyses to detect rotavirus in diarrheic fecal samples of calves. Can J Comp Med. 1979 Jan;43(1):84–89. [PMC free article] [PubMed] [Google Scholar]
  32. Rubenstein A. S., Miller M. F. Comparison of an enzyme immunoassay with electron microscopic procedures for detecting rotavirus. J Clin Microbiol. 1982 May;15(5):938–944. doi: 10.1128/jcm.15.5.938-944.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Sanekata T., Okada H. Human rotavirus detection by agglutination of antibody-coated erythrocytes. J Clin Microbiol. 1983 Jun;17(6):1141–1147. doi: 10.1128/jcm.17.6.1141-1147.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Sanekata T., Yoshida Y., Oda K. Detection of rotavirus from faeces by reversed passive haemagglutination method. J Clin Pathol. 1979 Sep;32(9):963–963. doi: 10.1136/jcp.32.9.963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Sanekata T., Yoshida Y., Okada H. Detection of rotavirus in faeces by latex agglutination. J Immunol Methods. 1981;41(3):377–385. doi: 10.1016/0022-1759(81)90199-x. [DOI] [PubMed] [Google Scholar]
  36. Sarkkinen H. K., Halonen P. E., Arstila P. P. Comparison of four-layer radioimmunoassay and electron microscopy for detection of human rotavirus. J Med Virol. 1979;4(4):255–260. doi: 10.1002/jmv.1890040403. [DOI] [PubMed] [Google Scholar]
  37. Sarkkinen H. K., Tuokko H., Halonen P. E. Comparison of enzyme-immunoassay and radioimmunoassay for detection of human rotaviruses and adenoviruses from stool specimens. J Virol Methods. 1980;1(6):331–341. doi: 10.1016/0166-0934(80)90050-6. [DOI] [PubMed] [Google Scholar]
  38. Sato K., Inaba Y., Shinozaki T., Fujii R., Matumoto M. Isolation of human rotavirus in cell cultures: brief report. Arch Virol. 1981;69(2):155–160. doi: 10.1007/BF01315159. [DOI] [PubMed] [Google Scholar]
  39. Skaug K., Figenschau K. J., Orstavik I. A rotavirus staphylococcal co-agglutination test. Acta Pathol Microbiol Immunol Scand B. 1983 Jun;91(3):175–178. doi: 10.1111/j.1699-0463.1983.tb00029.x. [DOI] [PubMed] [Google Scholar]
  40. Spence L., Fauvel M., Petro R., Bloch S. Comparison of Counterimmunoelectrophoresis and electron microscopy for laboratory diagnosis of human reovirus-like agent-associated infantile gastroenteritis. J Clin Microbiol. 1977 Feb;5(2):248–249. doi: 10.1128/jcm.5.2.248-249.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  41. Svensson L., Grandien M., Pettersson C. A. Comparison of solid-phase immune electron microscopy by use of protein A with direct electron microscopy and enzyme-linked immunosorbent assay for detection of rotavirus in stool. J Clin Microbiol. 1983 Nov;18(5):1244–1249. doi: 10.1128/jcm.18.5.1244-1249.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Tsuchie H., Shimase K., Tamura I., Kurimura O., Kaneto E., Katsumoto T., Ito M., Kurimura T. Comparison of enzyme-linked immunosorbent assay, electron microscopy, and reversed passive haemagglutination for detection of human rotavirus in stool specimens. Biken J. 1983 Jun;26(2):87–92. [PubMed] [Google Scholar]
  43. Vesikari T., Sarkkinen H. K., Mäki M. Quantitative aspects of rotavirus excretion in childhood diarrhoea. Acta Paediatr Scand. 1981 Sep;70(5):717–721. doi: 10.1111/j.1651-2227.1981.tb05774.x. [DOI] [PubMed] [Google Scholar]
  44. White L., Perez I., Perez M., Urbina G., Greenberg H., Kapikian A., Flores J. Relative frequency of rotavirus subgroups 1 and 2 in Venezuelan children with gastroenteritis as assayed with monoclonal antibodies. J Clin Microbiol. 1984 Apr;19(4):516–520. doi: 10.1128/jcm.19.4.516-520.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  45. Yolken R. H., Kim H. W., Clem T., Wyatt R. G., Kalica A. R., Chanock R. M., Kapikian A. Z. Enzyme-linked immunosorbent assay (ELISA) for detection of human reovirus-like agent of infantile gastroenteritis. Lancet. 1977 Aug 6;2(8032):263–267. doi: 10.1016/s0140-6736(77)90951-5. [DOI] [PubMed] [Google Scholar]
  46. Yolken R. H., Stopa P. J. Enzyme-linked fluorescence assay: Ultrasensitive solid-phase assay for detection of human rotavirus. J Clin Microbiol. 1979 Sep;10(3):317–321. doi: 10.1128/jcm.10.3.317-321.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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