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. 1984 Sep;20(3):509–514. doi: 10.1128/jcm.20.3.509-514.1984

Evaluation of the Quantum II and Rapid E identification systems.

P R Murray, A Gauthier, A Niles
PMCID: PMC271361  PMID: 6386866

Abstract

A total of 492 clinical isolates from the family Enterobacteriaceae were tested in the API 20E, Rapid E, and Quantum II identification systems. Discrepant identifications among these three systems were resolved by repeat testing in the identification systems or use of conventional biochemical tests. Of these isolates, 94.1% were correctly identified with the API 20E and Rapid E systems, and 97.0% were correctly identified with the Quantum II system. An additional 48 non-Enterobacteriaceae isolates were tested with the Quantum II system, and 83.3% were correctly identified. The majority of incorrect identifications with the Rapid E and Quantum II systems were caused by a single aberrant biochemical reaction. Reproducibility of the biochemical reactions obtained with these two systems was evaluated by testing 40 organisms in triplicate. Identical biocodes for all three tests were obtained for 10 organisms with the Quantum II system and for 19 organisms with the Rapid E system. Reproducibility of the Quantum II test results was improved with a subsequent modification of the photometer of this system. Both the Rapid E and Quantum II systems were inexpensive and were technically easy to inoculate and interpret.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E., Hodges R. L. Correlation studies of entero-set 20, API 20E and conventional media systems for Enterobacteriaceae identification. J Clin Microbiol. 1981 Jan;13(1):120–125. doi: 10.1128/jcm.13.1.120-125.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bale M. J., Matsen J. M. Time-motion and cost comparison study of micro-ID, API 20E, and conventional biochemical testing in identification of Enterobacteriaceae. J Clin Microbiol. 1981 Dec;14(6):665–670. doi: 10.1128/jcm.14.6.665-670.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barry A. L., Badal R. E., Effinger L. J. Identification of Enterobacteriaceae in frozen microdilution trays prepared by Micro-Media Systems. J Clin Microbiol. 1979 Oct;10(4):492–496. doi: 10.1128/jcm.10.4.492-496.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Barry A. L., Gavan T. L., Badal R. E., Telenson M. J. Sensitivity, specificity, and reproducibility of the automicrobic system (with the Enterobacteriaceae-plus biochemical card) for identifying clinical isolates of Gram- negative bacilli. J Clin Microbiol. 1982 Apr;15(4):582–588. doi: 10.1128/jcm.15.4.582-588.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Costigan W. J., Hollick G. E. Use of the Autobac IDX system for rapid identification of Enterobacteriaceae and nonfermentative gram-negative bacilli. J Clin Microbiol. 1984 Feb;19(2):301–302. doi: 10.1128/jcm.19.2.301-302.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edberg S. C., Atkinson B., Chambers C., Moore M. H., Palumbo L., Zorzon C. F., Singer J. M. Clinical evaluation of the MICRO-ID, API 20E, and conventional media systems for identification of Enterobacteriacea. J Clin Microbiol. 1979 Aug;10(2):161–167. doi: 10.1128/jcm.10.2.161-167.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Isenberg H. D., Gavan T. L., Smith P. B., Sonnenwirth A., Taylor W., Martin W. J., Rhoden D., Balows A. Collaborative investigation of the AutoMicrobic System Enterobacteriaceae biochemical card. J Clin Microbiol. 1980 Jun;11(6):694–702. doi: 10.1128/jcm.11.6.694-702.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kelly M. T., Latimer J. M. Comparison of the automicrobic system with API, enterotube, micro-ID, micro-media systems, and conventional methods for identification of Enterobacteriaceae. J Clin Microbiol. 1980 Nov;12(5):659–662. doi: 10.1128/jcm.12.5.659-662.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McCracken A. W., Martin W. J., McCarthy L. R., Schwab D. A., Cooper B. H., Helgeson N. G., Prowant S., Robson J. Evaluation of the MS-2 system for rapid identification of Enterobacteriaceae. J Clin Microbiol. 1980 Nov;12(5):684–689. doi: 10.1128/jcm.12.5.684-689.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Murray P. R. Standardization of the Analytab Enteric (API 20E) system to increase accuracy and reproducibility of the test for biotype characterization of bacteria. J Clin Microbiol. 1978 Jul;8(1):46–49. doi: 10.1128/jcm.8.1.46-49.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Staneck J. L., Vincelette J., Lamothe F., Polk E. A. Evaluation of the Sensititre system for identification of Enterobacteriaceae. J Clin Microbiol. 1983 Apr;17(4):647–654. doi: 10.1128/jcm.17.4.647-654.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Woolfrey B. F., Lally R. T., Quall C. O. Evaluation of the AutoSCAN-3 and Sceptor systems for Enterobacteriaceae identification. J Clin Microbiol. 1983 May;17(5):807–813. doi: 10.1128/jcm.17.5.807-813.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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