Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1984 Oct;20(4):618–623. doi: 10.1128/jcm.20.4.618-623.1984

Clinical laboratory comparison of the 10-ml isolator blood culture system with BACTEC radiometric blood culture media.

J A Kellogg, J P Manzella, J H McConville
PMCID: PMC271396  PMID: 6386871

Abstract

The efficiency of the 10-ml Isolator (E. I. du Pont de Nemours & Co., Inc.) for recovery of pathogens from blood was compared with that of BACTEC 6B and 7C media (Johnston Laboratories) by using 4,195 cultures from 1,662 patients. During the first phase of the study, BACTEC bottles were inoculated with 3 ml of blood; in the second phase, bottles were inoculated with 5 ml. The objectives were to compare results with similar blood volumes used for the detection of anaerobes as well as similar overall volumes and to determine the relative sensitivity of BACTEC media inoculated with the minimum and maximum volumes suggested by the manufacturer. From 180 patients, 391 significant isolates were recovered, 354 (91%) with the Isolator and 304 (78%) with the bottles. Isolators recovered 31 (15%) and 19 (18%) more pathogens overall than did the two-bottle system inoculated with 3 and 5 ml of blood, respectively, including 30 (36%) and 10 (34%) more Enterobacteriaceae. Recovery of anaerobes was greater in the BACTEC anaerobic medium, but only when its inoculum was increased to 5 ml. No significant differences existed between the two systems in pathogen detection times or detection of polymicrobic bacteremia. The Isolator contamination rate (8.3%) was approximately 4 times that of the bottles. The number of CFU of pathogen per milliliter of blood, blood volume sampled, and number of Isolators collected were more important than antimicrobial agent pretreatment in contributing to patient bacteremia of fungemia undetected by the Isolator. The Isolator appeared to be a practical alternative for recovery of aerobic and facultatively anaerobic pathogens from the blood.

Full text

PDF
618

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum P. C., Schick S. F., Kellogg J. A. Evaluation of the four-hour Micro-ID technique for direct identification of oxidase-negative, Gram-negative rods from blood cultures. J Clin Microbiol. 1980 Oct;12(4):533–537. doi: 10.1128/jcm.12.4.533-537.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Auckenthaler R., Ilstrup D. M., Washington J. A., 2nd Comparison of recovery of organisms from blood cultures diluted 10% (volume/volume) and 20% (volume/volume). J Clin Microbiol. 1982 May;15(5):860–864. doi: 10.1128/jcm.15.5.860-864.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bille J., Stockman L., Roberts G. D., Horstmeier C. D., Ilstrup D. M. Evaluation of a lysis-centrifugation system for recovery of yeasts and filamentous fungi from blood. J Clin Microbiol. 1983 Sep;18(3):469–471. doi: 10.1128/jcm.18.3.469-471.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dorn G. L., Haynes J. R., Burson G. G. Blood culture technique based on centrifugation: developmental phase. J Clin Microbiol. 1976 Mar;3(3):251–257. doi: 10.1128/jcm.3.3.251-257.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dorn G. L., Land G. A., Wilson G. E. Improved blood culture technique based on centrifugation: clinical evaluation. J Clin Microbiol. 1979 Mar;9(3):391–396. doi: 10.1128/jcm.9.3.391-396.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hall M. M., Ilstrup D. M., Washington J. A., 2nd Effect of volume of blood cultured on detection of bacteremia. J Clin Microbiol. 1976 Jun;3(6):643–645. doi: 10.1128/jcm.3.6.643-645.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Henry N. K., McLimans C. A., Wright A. J., Thompson R. L., Wilson W. R., Washington J. A., 2nd Microbiological and clinical evaluation of the isolator lysis-centrifugation blood culture tube. J Clin Microbiol. 1983 May;17(5):864–869. doi: 10.1128/jcm.17.5.864-869.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ilstrup D. M., Washington J. A., 2nd The importance of volume of blood cultured in the detection of bacteremia and fungemia. Diagn Microbiol Infect Dis. 1983 Jun;1(2):107–110. doi: 10.1016/0732-8893(83)90039-1. [DOI] [PubMed] [Google Scholar]
  9. Kelly M. T., Buck G. E., Fojtasek M. F. Evaluation of a lysis-centrifugation and biphasic bottle blood culture system during routine use. J Clin Microbiol. 1983 Sep;18(3):554–557. doi: 10.1128/jcm.18.3.554-557.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Kiehn T. E., Capitolo C., Armstrong D. Comparison of direct and standard microtiter broth dilution susceptibility testing of blood culture isolates. J Clin Microbiol. 1982 Jul;16(1):96–98. doi: 10.1128/jcm.16.1.96-98.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kiehn T. E., Wong B., Edwards F. F., Armstrong D. Comparative recovery of bacteria and yeasts from lysis-centrifugation and a conventional blood culture system. J Clin Microbiol. 1983 Aug;18(2):300–304. doi: 10.1128/jcm.18.2.300-304.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McLaughlin J. C., Hamilton P., Scholes J. V., Bartlett R. C. Clinical laboratory comparison of lysis-centrifugation and BACTEC radiometric blood culture techniques. J Clin Microbiol. 1983 Nov;18(5):1027–1031. doi: 10.1128/jcm.18.5.1027-1031.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Moore D. F., Hamada S. S., Marso E., Martin W. J. Rapid identification and antimicrobial susceptibility testing of gram-negative bacilli from blood cultures by the AutoMicrobic system. J Clin Microbiol. 1981 May;13(5):934–939. doi: 10.1128/jcm.13.5.934-939.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Salventi J. F., Davies T. A., Randall E. L., Whitaker S., Waters J. R. Effect of blood dilution on recovery of organisms from clinical blood cultures in medium containing sodium polyanethol sulfonate. J Clin Microbiol. 1979 Feb;9(2):248–252. doi: 10.1128/jcm.9.2.248-252.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES