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. 1984 Oct;20(4):653–659. doi: 10.1128/jcm.20.4.653-659.1984

Glucose-sucrose-potassium tellurite-bacitracin agar, an alternative to mitis salivarius-bacitracin agar for enumeration of Streptococcus mutans.

J M Tanzer, A C Börjesson, L Laskowski, A B Kurasz, M Testa
PMCID: PMC271404  PMID: 6490852

Abstract

An agar medium for selective recovery and enumeration of Streptococcus mutans was developed as an alternative to mitis salivarius-bacitracin (MSB) agar. Combinations of dyes, antibiotics, and tellurite were added to a nonselective medium which, because of its sucrose content, allowed easy recognition of S. mutans colonies. Candle jar incubation for 2 days, by comparison with anaerobic incubation, reduced background flora but did not diminish S. mutans recoveries from clinical samples. Quantitative comparisons were made of the simultaneous recoveries of a number of authentic S. mutans serotype representatives and fresh clinical isolates, using various glucose-sucrose-potassium tellurite-bacitracin (GSTB) formulations and mitis salivarius, MSB, and blood agars. Mitis salivarius counts were not detectably different from blood counts, but counts on MSB were distinctly lower. A formulation of the new medium containing 5% glucose 5% sucrose, 0.001% potassium tellurite, 0.3 U of bacitracin per ml (hence GSTB), and 2% agar gave recoveries nearly equal to those on mitis salivarius agar and much greater than those on MSB. The medium yielded readily recognized S. mutans colonies and facilitated detection of intracellular polysaccharide formers upon flooding with I2 reagent. Freshly isolated serotype c, E, and f colonies could often be distinguished from serotype d and g colonies, a distinction made reliable by testing for intracellular polysaccharide. A study of 300 salivary samples revealed GSTB to give significantly higher recoveries than MSB. About 72% of all samples were substantially underestimated for S. mutans with MSB, and 6.7% of samples were falsely negative for S. mutans with MSB. Recovery of background flora on GSTB was as low or lower than on MSB, and both types of agar could be stored for at least 9 weeks without notable change of selectivity. Thus, GSTB agar appears to be simple and reliable to use and requires no anaerobic incubation. Caution is voiced about interpretation of data previously reported which evaluated S. mutans on MSB agar.

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Selected References

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  1. Bratthall D. Demonstration of five serological groups of streptococcal strains resembling Streptococcus mutans. Odontol Revy. 1970;21(2):143–152. [PubMed] [Google Scholar]
  2. Carlsson J. A medium for isolation of Streptococcus mutans. Arch Oral Biol. 1967 Dec;12(12):1657–1658. doi: 10.1016/0003-9969(67)90201-4. [DOI] [PubMed] [Google Scholar]
  3. De Stoppelaar J. D., Van Houte J., Backer Dirks O. The relationship between extracellular polysaccharide-producing streptococci and smooth surface caries in 13-year-old children. Caries Res. 1969;3(2):190–199. doi: 10.1159/000259582. [DOI] [PubMed] [Google Scholar]
  4. Duchin S., van Houte J. Relationship of Streptococcus mutans and lactobacilli to incipient smooth surface dental caries in man. Arch Oral Biol. 1978;23(9):779–786. doi: 10.1016/0003-9969(78)90155-3. [DOI] [PubMed] [Google Scholar]
  5. Ellen R. P., Fillery E. D., Banting D. W. Comparison of selective broth and plating methods for isolation of Streptococcus mutans from root surface dental plaques. J Clin Microbiol. 1980 Mar;11(3):205–208. doi: 10.1128/jcm.11.3.205-208.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Emilson C. G., Axelsson P., Kallenberg L. Effect of mechanical and chemical plaque control measures on oral microflora in schoolchildren. Community Dent Oral Epidemiol. 1982 Jun;10(3):111–116. doi: 10.1111/j.1600-0528.1982.tb01333.x. [DOI] [PubMed] [Google Scholar]
  7. Emilson C. G., Bratthall D. Growth of Streptococcus mutans on various selective media. J Clin Microbiol. 1976 Jul;4(1):95–98. doi: 10.1128/jcm.4.1.95-98.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. FITZGERALD R. J., KEYES P. H. Demonstration of the etiologic role of streptococci in experimental caries in the hamster. J Am Dent Assoc. 1960 Jul;61:9–19. doi: 10.14219/jada.archive.1960.0138. [DOI] [PubMed] [Google Scholar]
  9. Fitzgerald R. F., Adams B. O. Increased selectivity of Mitis-Salivarius agar containing polymyxin. J Clin Microbiol. 1975 Feb;1(2):239–240. doi: 10.1128/jcm.1.2.239-240.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Freedman M. L., Coykendall A. L. Variation in internal polysaccharide synthesis among Streptococcus mutans strains. Infect Immun. 1975 Sep;12(3):475–479. doi: 10.1128/iai.12.3.475-479.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gold O. G., Jordan H. V., Van Houte J. A selective medium for Streptococcus mutans. Arch Oral Biol. 1973 Nov;18(11):1357–1364. doi: 10.1016/0003-9969(73)90109-x. [DOI] [PubMed] [Google Scholar]
  12. Ikeda T., Sandham H. J. A high-sucrose medium for the identification of Streptococcus mutans. Arch Oral Biol. 1972 Apr;17(4):781–783. doi: 10.1016/0003-9969(72)90204-x. [DOI] [PubMed] [Google Scholar]
  13. Ikeda T., Sandham H. J. Prevalence of Streptococcus mutans on various tooth surfaces in Negro children. Arch Oral Biol. 1971 Oct;16(10):1237–1240. doi: 10.1016/0003-9969(71)90053-7. [DOI] [PubMed] [Google Scholar]
  14. JORDAN H. V., FITZGERALD R. J., BOWLER A. E. Inhibition of experimental caries by sodium metabisulfite and its effect on the growth and metabolism of selected bacteria. J Dent Res. 1960 Jan-Feb;39:116–123. doi: 10.1177/00220345600390010501. [DOI] [PubMed] [Google Scholar]
  15. Klock B., Krasse B. Effect of caries-preventive measures in children with high numbers of S. mutans and lactobacilli. Scand J Dent Res. 1978 Jul;86(4):221–230. doi: 10.1111/j.1600-0722.1978.tb00622.x. [DOI] [PubMed] [Google Scholar]
  16. Köhler B., Bratthall D. Intrafamilial levels of Streptococcus mutans and some aspects of the bacterial transmission. Scand J Dent Res. 1978 Jan;86(1):35–42. doi: 10.1111/j.1600-0722.1978.tb00605.x. [DOI] [PubMed] [Google Scholar]
  17. Köhler B., Bratthall D., Krasse B. Preventive measures in mothers influence the establishment of the bacterium Streptococcus mutans in their infants. Arch Oral Biol. 1983;28(3):225–231. doi: 10.1016/0003-9969(83)90151-6. [DOI] [PubMed] [Google Scholar]
  18. Linke H. A. New method for the isolation of Streptococcus mutans and its differentiation from other oral streptococci. J Clin Microbiol. 1977 Jun;5(6):604–609. doi: 10.1128/jcm.5.6.604-609.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Little W. A., Korts D. C., Thomson L. A., Bowen W. H. Comparative recovery of Streptococcus mutans on ten isolation media. J Clin Microbiol. 1977 Jun;5(6):578–583. doi: 10.1128/jcm.5.6.578-583.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Littleton N. W., Kakehashi S., Fitzgerald R. J. Recovery of specific "caries-inducing" streptococci from carious lesions in the teeth of children. Arch Oral Biol. 1970 May;15(5):461–463. doi: 10.1016/0003-9969(70)90073-7. [DOI] [PubMed] [Google Scholar]
  21. Möller A. J. Microbiological examination of root canals and periapical tissues of human teeth. Methodological studies. Odontol Tidskr. 1966 Dec 20;74(5 Suppl):1–380. [PubMed] [Google Scholar]
  22. Perch B., Kjems E., Ravn T. Biochemical and serological properties of Streptococcus mutans from various human and animal sources. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Jun;82(3):357–370. doi: 10.1111/j.1699-0463.1974.tb02338.x. [DOI] [PubMed] [Google Scholar]
  23. Svanberg M., Loesche W. J. The salivary concentration of Streptococci mutans and Streptococci sanguis and their colonization of artificial tooth fissures in man. Arch Oral Biol. 1977;22(7):441–447. doi: 10.1016/0003-9969(77)90125-x. [DOI] [PubMed] [Google Scholar]
  24. Syed S. A., Loesche W. J. Efficiency of various growth media in recovering oral bacterial flora from human dental plaque. Appl Microbiol. 1973 Oct;26(4):459–465. doi: 10.1128/am.26.4.459-465.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Tanzer J. M., Fisher J., Freedman M. L. Preemption of Streptococcus mutans 10449S colonization by its mutant 805. Infect Immun. 1982 Jan;35(1):138–142. doi: 10.1128/iai.35.1.138-142.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tanzer J. M., Freedman M. L., Fitzgerald R. J., Larson R. H. Diminished virulence of glucan synthesis-defective mutants of Streptococcus mutans. Infect Immun. 1974 Jul;10(1):197–203. doi: 10.1128/iai.10.1.197-203.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Van Palenstein Helderman W. H., Ijsseldijk M., Huis in 't Veld J. H. A selective medium for the two major subgroups of the bacterium Streptococcus mutans isolated from human dental plaque and saliva. Arch Oral Biol. 1983;28(7):599–603. doi: 10.1016/0003-9969(83)90007-9. [DOI] [PubMed] [Google Scholar]
  28. Westergren G., Krasse B. Evaluation of a micromethod for determination of Streptococcus mutans and Lactobacillus infection. J Clin Microbiol. 1978 Jan;7(1):82–83. doi: 10.1128/jcm.7.1.82-83.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Zickert I., Emilson C. G., Krasse B. Streptococcus mutans, lactobacilli and dental health in 13-14-year-old Swedish children. Community Dent Oral Epidemiol. 1982 Apr;10(2):77–81. doi: 10.1111/j.1600-0528.1982.tb00367.x. [DOI] [PubMed] [Google Scholar]

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