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. 1984 Oct;20(4):660–663. doi: 10.1128/jcm.20.4.660-663.1984

Comparison of indirect hemagglutination and rapid plate agglutination tests with counterimmunoelectrophoresis for typing Pasteurella haemolytica.

M M Chengappa, G R Carter, W E Bailie
PMCID: PMC271405  PMID: 6490853

Abstract

A rapid, simple, and accurate counterimmunoelectrophoresis (CIE) technique was developed and compared with the indirect hemagglutination and rapid plate agglutination techniques for serotyping cultures of Pasteurella haemolytica. The CIE test had 100% correlation with the conventional indirect hemagglutination test and, after serum absorption, correctly identified cultures representing the 12 established serotypes and 49 field isolates of P. haemolytica with reasonable rapidity. Cross-reactions were observed in the CIE and rapid plate agglutination tests but not in the indirect hemagglutination test with antisera prepared from the 12 established serotypes. These cross-reactions were eliminated from the CIE test but not from the rapid plate agglutination test by absorption of antisera with cells which possessed the cross-reacting antigens. Avian isolates of P. haemolytica did not type with antisera to the 12 established serotypes by any of the methods. Both homologous and heterologous reactions were observed with these strains in the rapid plate agglutination and CIE tests with antisera prepared from six selected cultures. These results support the previous finding that the taxonomic relationship of these avian strains to P. haemolytica is questionable.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BIBERSTEIN E. L., GILLS M. G. The relation of the antigenic types to the A and T types of Pasteurella haemolytica. J Comp Pathol. 1962 Jul;72:316–320. doi: 10.1016/s0368-1742(62)80036-8. [DOI] [PubMed] [Google Scholar]
  2. BIBERSTEIN E. L., GILLS M., KNIGHT H. Serological types of Pasteurella hemolytica. Cornell Vet. 1960 Jul;50:283–300. [PubMed] [Google Scholar]
  3. Biberstein E. L., Shreeve B. J., Thompson D. A. Variation in carrier rates of Pasteurella haemolytica in sheep flocks. I. Normal flocks. J Comp Pathol. 1970 Oct;80(4):499–507. doi: 10.1016/0021-9975(70)90046-0. [DOI] [PubMed] [Google Scholar]
  4. Carter G. R., Chengappa M. M. Identification of types B and E Pasteurella multocida by counterimmunoelectrophoresis. Vet Rec. 1981 Feb 14;108(7):145–146. doi: 10.1136/vr.108.7.145. [DOI] [PubMed] [Google Scholar]
  5. Cho H. J., Greenfield J. Eradication of Aleutian disease of mink by eliminating positive counterimmunoelectrophoresis test reactors. J Clin Microbiol. 1978 Jan;7(1):18–22. doi: 10.1128/jcm.7.1.18-22.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Frank G. H., Wessman G. E. Rapid plate agglutination procedure for serotyping Pasteurella haemolytica. J Clin Microbiol. 1978 Feb;7(2):142–145. doi: 10.1128/jcm.7.2.142-145.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fraser J., Donachie W., Quirie M., Gilmour N. J. Rapid indirect hemagglutination test for serotyping Pasteurella haemolytica. J Clin Microbiol. 1983 Jul;18(1):206–207. doi: 10.1128/jcm.18.1.206-207.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Fraser J., Laird S., Gilmour N. J. A new serotype (biotype T) of Pasteurella haemolytica. Res Vet Sci. 1982 Jan;32(1):127–128. [PubMed] [Google Scholar]
  9. Ingram D. L., Anderson P., Smith D. H. Countercurrent immunoelectrophoresis in the diagnosis of systemic diseases caused by Hemophilus infleunzae type b. J Pediatr. 1972 Dec;81(6):1156–1159. doi: 10.1016/s0022-3476(72)80252-x. [DOI] [PubMed] [Google Scholar]
  10. Ingram D. L., Collier A. M., Pendergrass E., King S. H. Methods for serotyping nasopharyngeal isolates of Haemophilus influenzae: slide agglutination, Quellung reaction, countercurrent immunoelectrophoresis, latex agglutination, and antiserum agar. J Clin Microbiol. 1979 May;9(5):570–574. doi: 10.1128/jcm.9.5.570-574.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pegram R. G., Roeder P. L., Scott J. M. Two new serotypes of Pasteurella haemolytica from sheep in Ethiopia. Trop Anim Health Prod. 1979 Feb;11(1):29–30. doi: 10.1007/BF02237762. [DOI] [PubMed] [Google Scholar]
  12. Shackelford P. G., Campbell J., Feigin R. D. Countercurrent immunoelectrophoresis in the evaluation of childhood infections. J Pediatr. 1974 Oct;85(4):478–481. doi: 10.1016/s0022-3476(74)80448-8. [DOI] [PubMed] [Google Scholar]
  13. Shreeve B. J., Ivanov I. N., Thompson D. A. Biochemical reactions of different serotypes of Pasteurella haemolytica. J Med Microbiol. 1970 May;3(2):356–358. doi: 10.1099/00222615-3-2-356. [DOI] [PubMed] [Google Scholar]

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