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. 1984 Oct;20(4):696–700. doi: 10.1128/jcm.20.4.696-700.1984

Use of bone marrow fibroblasts to prepare targets for an HLA restricted-cytotoxicity assay system.

R A Bowden, L McGavren, A R Hayward, M J Levin
PMCID: PMC271414  PMID: 6490855

Abstract

Methods are described for obtaining and growing fibroblasts from bone marrow for use as virus-infected targets. Fibroblasts obtained at the time of routine marrow examination were maintained through 12 to 18 passages as confluent monolayers. Bone marrow fibroblasts could be infected with varicella-zoster virus, and these infected cells were suitable targets for a 51Cr-release cytotoxicity assay. Since these virus-infected cells retain their HLA-A and -B antigens, they are readily available to study the immune cells which mediate virus-specific cytotoxicity.

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Selected References

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