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. 1984 Oct;20(4):802–805. doi: 10.1128/jcm.20.4.802-805.1984

Enzyme immunoassay for detection of pneumococcal antigen in cerebrospinal fluid.

R H Yolken, D Davis, J Winkelstein, H Russell, J E Sippel
PMCID: PMC271434  PMID: 6386883

Abstract

A solid-phase immunoassay utilizing horse antiserum against the C polysaccharide of Streptococcus pneumoniae and biotinylated rabbit antibodies to type-specific pneumococcal polysaccharides was developed to detect pneumococcal antigens in human body fluids and in broth cultures. Pneumococcal antigen could be detected in broth cultures of serotypes of S. pneumoniae containing as little as 10(2) to 10(3) organisms per ml. The assay system detected pneumococcal antigen in all 25 cerebrospinal fluid specimens obtained from patients with documented pneumococcal meningitis. There were no positive reactions noted in specimens from patients infected with Neisseria meningitidis group A or from patients without evidence of bacterial infection. The solid-phase enzyme immunoassay utilizing these reagents is a sensitive and specific assay for the immunodetection of a wide range of pneumococcal antigens.

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Selected References

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  1. AUSTRIAN R., GOLD J. PNEUMOCOCCAL BACTEREMIA WITH ESPECIAL REFERENCE TO BACTEREMIC PNEUMOCOCCAL PNEUMONIA. Ann Intern Med. 1964 May;60:759–776. doi: 10.7326/0003-4819-60-5-759. [DOI] [PubMed] [Google Scholar]
  2. Barrett-Connor E. Bacterial infection and sickle cell anemia. An analysis of 250 infections in 166 patients and a review of the literature. Medicine (Baltimore) 1971 Mar;50(2):97–112. [PubMed] [Google Scholar]
  3. Bayer E., Wilchek M. Insolubilized biotin for the purification of avidin. Methods Enzymol. 1974;34:265–267. doi: 10.1016/s0076-6879(74)34023-2. [DOI] [PubMed] [Google Scholar]
  4. CONE L., UHR J. W. IMMUNOLOGICAL DEFICIENCY DISORDERS ASSOCIATED WITH CHRONIC LYMPHOCYTIC LEUKEMIA AND MULTIPLE MYELOMA. J Clin Invest. 1964 Dec;43:2241–2248. doi: 10.1172/JCI105098. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cooksey R. C., Facklam R. R., Thornsberry C. Antimicrobial susceptibility patterns of Streptococcus pneumoniae. Antimicrob Agents Chemother. 1978 Apr;13(4):645–648. doi: 10.1128/aac.13.4.645. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fine D. P. Pneumococcal type-associated variability in alternate complement pathway activation. Infect Immun. 1975 Oct;12(4):772–778. doi: 10.1128/iai.12.4.772-778.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fossieck B., Jr, Craig R., Paterson P. Y. Counterimmunoelectrophoresis for rapid diagnosis of meningitis due to Diplococcus pneumoniae. J Infect Dis. 1973 Jan;127(1):106–109. doi: 10.1093/infdis/127.1.106. [DOI] [PubMed] [Google Scholar]
  8. Gotschlich E. C., Liu T. Y. Structural and immunological studies on the pneumococcal C polysaccharide. J Biol Chem. 1967 Feb 10;242(3):463–470. [PubMed] [Google Scholar]
  9. Herrmann J. E., Collins M. F. Quantitation of immunoglobulin adsorption to plastics. J Immunol Methods. 1976;10(4):363–366. doi: 10.1016/0022-1759(76)90030-2. [DOI] [PubMed] [Google Scholar]
  10. LIU T. Y., GOTSCHLICH E. C. The chemical composition of pneumococcal C-polysaccharide. J Biol Chem. 1963 Jun;238:1928–1934. [PubMed] [Google Scholar]
  11. Larm O., Lindberg B. The pneumococcal polysaccharides: a re-examination. Adv Carbohydr Chem Biochem. 1976;33:295–322. doi: 10.1016/s0065-2318(08)60284-x. [DOI] [PubMed] [Google Scholar]
  12. Lehtonen O. P., Viljanen M. K. Antigen attachment in ELISA. J Immunol Methods. 1980;34(1):61–70. doi: 10.1016/0022-1759(80)90225-2. [DOI] [PubMed] [Google Scholar]
  13. Place J. D., Schroeder H. R. The fixation of anti-HBsAg on plastic surfaces. J Immunol Methods. 1982;48(2):251–260. doi: 10.1016/0022-1759(82)90199-5. [DOI] [PubMed] [Google Scholar]
  14. Robinson M. G., Watson R. J. Pneumococcal meningitis in sickle-cell anemia. N Engl J Med. 1966 May 5;274(18):1006–1008. doi: 10.1056/NEJM196605052741806. [DOI] [PubMed] [Google Scholar]
  15. Rytel M. W. Rapid diagnostic methods in infectious diseases. Adv Intern Med. 1975;20:37–60. [PubMed] [Google Scholar]
  16. Schiffman G., Bornstein D. L., Austrian R. Capsulation of pneumococcus with soluble cell wall-like polysaccharide. II. Nonidentity of cell wall and soluble cell wall-like polysaccharides derived from the same and from different pneumococcal strains. J Exp Med. 1971 Sep 1;134(3 Pt 1):600–617. doi: 10.1084/jem.134.3.600. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sippel J. E., Prato C. M., Girgis N. I., Edwards E. A. Detection of Neisseria meningitidis group A, Haemophilus influenzae type b, and Streptococcus pneumoniae antigens in cerebrospinal fluid specimens by antigen capture enzyme-linked immunosorbent assays. J Clin Microbiol. 1984 Aug;20(2):259–265. doi: 10.1128/jcm.20.2.259-265.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Smith M. R., Wood W. B., Jr Heat labile opsonins to pneumococcus. I. Participation of complement. J Exp Med. 1969 Dec 1;130(6):1209–1227. doi: 10.1084/jem.130.6.1209. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tomasz A. Surface components of Streptococcus pneumoniae. Rev Infect Dis. 1981 Mar-Apr;3(2):190–211. doi: 10.1093/clinids/3.2.190. [DOI] [PubMed] [Google Scholar]
  20. Yolken R. H. Enzyme immunoassays for the detection of infectious antigens in body fluids: current limitations and future prospects. Rev Infect Dis. 1982 Jan-Feb;4(1):35–68. doi: 10.1093/clinids/4.1.35. [DOI] [PubMed] [Google Scholar]
  21. Yolken R. H., Leister F. J., Whitcomb L. S., Santosham M. Enzyme immunoassays for the detection of bacterial antigens utilizing biotin-labeled antibody and peroxidase biotin--avidin complex. J Immunol Methods. 1983 Feb 11;56(3):319–327. doi: 10.1016/s0022-1759(83)80021-0. [DOI] [PubMed] [Google Scholar]
  22. Yolken R. H., Stopa P. J. Analysis of nonspecific reactions in enzyme-linked immunosorbent assay testing for human rotavirus. J Clin Microbiol. 1979 Nov;10(5):703–707. doi: 10.1128/jcm.10.5.703-707.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]

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