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. 1984 Nov;20(5):877–880. doi: 10.1128/jcm.20.5.877-880.1984

Analysis of Pneumocystis carinii cysts with a fluorescence-activated cell sorter.

C R Libertin, G E Woloschak, W R Wilson, T F Smith
PMCID: PMC271464  PMID: 6392322

Abstract

Human sera from Pneumocystis carinii-infected patients and specific rabbit antisera have antibodies against the cyst form of the organism. Lung tissue concentrations from cortisone-treated C3H/HeN mice and six open lung biopsy-positive patients were centrifuged and suspended, and immunofluorescent staining was done. We utilized the fluorescence-activated cell sorter to analyze and sort P. carinii cysts from lung homogenates into a morphologically distinct population. A quantitative basis was used for the definition of the cyst population by displaying the frequency of cells as a function of parameter (fluorescence intensity and light scatter) expression. In 14 of 15 histogram analyses, P. carinii-infected homogenates were differentiated from normal- and bacterial-control homogenates. The parameter range of light scatter (size) was 2 to 8 micron, and the fluorescence intensity was greater than a threshold based on the histogram profile.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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