FIGURE 2.

Sex-dependent expression profiles of miR-29b and miR-143 in mouse embryos. (A) Fractions containing small RNAs isolated from male (M) or female (F) mouse gonads at E13.5, E15.5, or E17.5 were subjected to Northern blot analysis with probes specific for miR-29b, miR-143, or U6 RNA. (Closed and open arrowheads) The positions corresponding to 24 and 19 nt, respectively. (B) The hybridization signal intensity for each miRNA in A was normalized by that of U6 RNA and plotted against embryonic stage.