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. 1984 Nov;20(5):1020–1021. doi: 10.1128/jcm.20.5.1020-1021.1984

Simple method for collagenase determination in 38 Pseudomonas aeruginosa strains.

G Wellisch, E Cohen, Z Cahane, J Horowitz
PMCID: PMC271504  PMID: 6096395

Abstract

Collagenase enzyme activity of 38 Pseudomonas aeruginosa strains and 38 strains of Escherichia coli from various pathological sources was measured by a simple method. This method uses plates with collagen gel. The rate of gel lysis is proportional to the collagenase concentration. The method is simple and requires no special materials or equipment. From the 38 P. aeruginosa strains, 34 were collagenase positive. All 38 strains of E. coli were collagenase negative.

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Selected References

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  1. Berman M. B., Manabe R., Davison P. F. Tissue collagenase: a simplified, semiquantitative enzyme assay. Anal Biochem. 1973 Aug;54(2):522–534. doi: 10.1016/0003-2697(73)90384-9. [DOI] [PubMed] [Google Scholar]
  2. Beutner E. H., Triftshauser C., Hazen S. P. Collagenase activity of gingival tissue from patients with periodontal disease. Proc Soc Exp Biol Med. 1966 Apr;121(4):1082–1085. doi: 10.3181/00379727-121-30972. [DOI] [PubMed] [Google Scholar]
  3. Dayer J. M., Krane S. M., Russell R. G., Robinson D. R. Production of collagenase and prostaglandins by isolated adherent rheumatoid synovial cells. Proc Natl Acad Sci U S A. 1976 Mar;73(3):945–949. doi: 10.1073/pnas.73.3.945. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Diener B., Carrick L., Jr, Berk R. S. In vivo studies with collagenase from Pseudomonas aeruginosa. Infect Immun. 1973 Feb;7(2):212–217. doi: 10.1128/iai.7.2.212-217.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. GROSS J., LAPIERE C. M. Collagenolytic activity in amphibian tissues: a tissue culture assay. Proc Natl Acad Sci U S A. 1962 Jun 15;48:1014–1022. doi: 10.1073/pnas.48.6.1014. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Labrosse K. R., Liener I. E., Hargrave P. A. A sensitive assay for collagenolytic activity using tritiated collagen. Anal Biochem. 1976 Jan;70(1):218–223. doi: 10.1016/s0378-5173(83)90103-5. [DOI] [PubMed] [Google Scholar]
  7. Levenson R. A collagen film microassay for tissue collagenase. Anal Biochem. 1976 Dec;76(2):579–588. doi: 10.1016/0003-2697(76)90352-3. [DOI] [PubMed] [Google Scholar]
  8. MANDL I., KELLER S., MANAHAN J. MULTIPLICITY OF CLOSTRIDIUM HISTOLYTICUM COLLAGENASES. Biochemistry. 1964 Nov;3:1737–1741. doi: 10.1021/bi00899a026. [DOI] [PubMed] [Google Scholar]
  9. Morihara K., Tsuzuki H. Production of protease and elastase by Pseudomonas aeruginosa strains isolated from patients. Infect Immun. 1977 Mar;15(3):679–685. doi: 10.1128/iai.15.3.679-685.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nagai Y., Lapiere C. M., Gross J. Tadpole collagenase. Preparation and purification. Biochemistry. 1966 Oct;5(10):3123–3130. doi: 10.1021/bi00874a007. [DOI] [PubMed] [Google Scholar]
  11. Steere A. C., Brinckerhoff C. E., Miller D. J., Drinker H., Harris E. D., Jr, Malawista S. E. Elevated levels of collagenase and prostaglandin E2 from synovium associated with erosion of cartilage and bone in a patient with chronic Lyme arthritis. Arthritis Rheum. 1980 May;23(5):591–599. doi: 10.1002/art.1780230511. [DOI] [PubMed] [Google Scholar]
  12. Terato K., Nagai Y., Kawanishi K., Yamamoto S. A rapid assay method of collagenase activity using 14C-labeled soluble collagen as substrate. Biochim Biophys Acta. 1976 Oct 11;445(3):753–762. doi: 10.1016/0005-2744(76)90125-x. [DOI] [PubMed] [Google Scholar]
  13. Waldvogel F. A., Swartz M. N. Collagenolytic activity of bacteria. J Bacteriol. 1969 May;98(2):662–667. doi: 10.1128/jb.98.2.662-667.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]

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