Table 2.
Selected RR band assignments for the CO and H2S-HbI mutant derivatives.
| Protein | CO | H2S | |||
|---|---|---|---|---|---|
| νFe-CO | νc-o | ν2 | ν3 | ν4 | |
| WtHbIa | 516 | 1945 | 1579 | 1500 | 1372 |
| rHbI | 516 | 1945 | 1583 | 1504, 1470 | 1374 (87%)b |
| 1356 (13%) | |||||
| ValE7 | 504 | 1962 | 1580 | 1501 | 1372 |
| AsnE7 | 507 | 1946 | nd | nd | nd |
| HisE7 | 502 | 1965 | 1583 | 1505, 1470 | 1373 (53%) |
| 532 | 1930 | 1356 (47%) | |||
| LeuB10 | 504 | 1944 | 1583 | 1502, 1470 | 1373 (53%) |
| 1355 (47%) | |||||
| ValB10 | 504 | 1946 | 1582, 1562 | 1500, 1470 | 1372 (32%) |
| 1355 (68%) | |||||
| TyrB10 | 541 | 1925 | 1583 | 1503. 1470 | 1372 (80%) |
| 1354 (20%) | |||||
| ValE11 | 514 | 1944 | 1581 | 1502, 1470 | 1373 (70%) |
| 1355 (30%) | |||||
| TyrE11 | 514 | 1940 | 1581 | 1502, 1470 | 1373 (86%) |
| 1356 (14%) | |||||
a
Data taken from reference reference 31.
b
The RR spectra were decomposed using peak fitting software from Grams spectra. The relative intensity of each band was calculated by the ratio of their height to the total relative height. nd, not determined.