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. 1984 Dec;20(6):1213–1215. doi: 10.1128/jcm.20.6.1213-1215.1984

Evaluation of focus reduction neutralization test with peroxidase-antiperoxidase staining technique for hemorrhagic fever with renal syndrome virus.

O Tanishita, Y Takahashi, Y Okuno, K Yamanishi, M Takahashi
PMCID: PMC271555  PMID: 6440908

Abstract

Titers of hemorrhagic fever with renal syndrome virus were estimated by counting foci stained with peroxidase-antiperoxidase or by using immunofluorescence methods. Foci stained with PAP were clearer and easier to count. The peroxidase-antiperoxidase method showed a linear relationship between virus concentration and the number of foci. A focus-reduction neutralizing antibody test was performed with immune rat sera and sera from patients. Again, the peroxidase-antiperoxidase method proved to be more convenient and reliable than the immunofluorescence method. Antigenic differences between Hantaan virus, 76-118 and hemorrhagic fever with renal syndrome virus B-1 were clearly demonstrated by the neutralizing antibody test.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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