Abstract
Two fully automated drug-monitoring systems, the Syva Autolab 6000 and the Beckman Auto ICS, were compared in terms of accuracy, precision, speed of operation, and cost effectiveness in the determination of gentamicin levels in serum. Within-run and between-run precision for both systems were acceptable (coefficient of variation, 2.0 to 6.9%), and patient sample comparisons resulted in an intermethod correlation coefficient of 0.96. When reference samples (prepared to contain 1.2 to 10 micrograms of gentamicin per ml) were assayed, the Syva Autolab 6000 obtained concentrations within 8% of expected values, whereas the Beckman Auto ICS reported values up to 17% higher than target values. In a time and cost comparison, reagent costs for the Beckman system were ca. 50% less than for the Syva Autolab 6000; the Syva system, however, determined patient results two to three times faster than the Beckman Auto ICS.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Appel G. B., Neu H. C. Gentamicin in 1978. Ann Intern Med. 1978 Oct;89(4):528–538. doi: 10.7326/0003-4819-89-4-528. [DOI] [PubMed] [Google Scholar]
- Barza M., Lauermann M. Why monitor serum levels of gentamicin? Clin Pharmacokinet. 1978 May-Jun;3(3):202–215. doi: 10.2165/00003088-197803030-00002. [DOI] [PubMed] [Google Scholar]
- Buchanan A. G., Witwicki E., Albritton W. L. Serum aminoglycoside monitoring by enzyme immunoassay, biological, and fluorescence immunoassay procedures. Am J Med Technol. 1983 Jun;49(6):437–441. [PubMed] [Google Scholar]
- Delaney C. J., Opheim K. E., Smith A. L., Plorde J. J. Performance characteristics of bioassay, radioenzymatic assay, homogeneous enzyme immunoassay, and high-performance liquid chromatographic determination of serum gentamicin. Antimicrob Agents Chemother. 1982 Jan;21(1):19–25. doi: 10.1128/aac.21.1.19. [DOI] [PMC free article] [PubMed] [Google Scholar]
- O'Leary T. D., Ratcliff R. M., Geary T. D. Evaluation of an enzyme immunoassay for serum gentamicin. Antimicrob Agents Chemother. 1980 May;17(5):776–778. doi: 10.1128/aac.17.5.776. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Phaneuf D., Francke E., Neu H. C. Rapid, reproducible enzyme immunoassay for gentamicin. J Clin Microbiol. 1980 Mar;11(3):266–269. doi: 10.1128/jcm.11.3.266-269.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ratcliff R. M., Mirelli C., Moran E., O'Leary D., White R. Comparison of five methods for the assay of serum gentamicin. Antimicrob Agents Chemother. 1981 Apr;19(4):508–512. doi: 10.1128/aac.19.4.508. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ristuccia A. M., Cunha B. A. The aminoglycosides. Med Clin North Am. 1982 Jan;66(1):303–312. doi: 10.1016/s0025-7125(16)31462-6. [DOI] [PubMed] [Google Scholar]
- Siber G. R., Echeverria P., Smith A. L., Paisley J. W., Smith D. H. Pharmacokinetics of gentamicin in children and adults. J Infect Dis. 1975 Dec;132(6):637–651. doi: 10.1093/infdis/132.6.637. [DOI] [PubMed] [Google Scholar]
- White L. O., Scammell L. M., Reeves D. S. Serum aminoglycoside assay by enzyme-mediated immunoassay (EMIT): correlation with radioimmunoassay, microbiological assays. Antimicrob Agents Chemother. 1981 Jun;19(6):1064–1066. doi: 10.1128/aac.19.6.1064. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Witebsky F. G., Sliva C. A., Selepak S. T., Ruddel M. E., MacLowry J. D., Johnson E. E., Elin R. J. Evaluation of four gentamicin and tobramycin assay procedures for clinical laboratories. J Clin Microbiol. 1983 Oct;18(4):890–894. doi: 10.1128/jcm.18.4.890-894.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaske D. E., Cipolle R. J., Rotschafer J. C., Solem L. D., Mosier N. R., Strate R. G. Gentamicin pharmacokinetics in 1,640 patients: method for control of serum concentrations. Antimicrob Agents Chemother. 1982 Mar;21(3):407–411. doi: 10.1128/aac.21.3.407. [DOI] [PMC free article] [PubMed] [Google Scholar]