Abstract
Several of the 41 types of human adenovirus have been divided into genome types based on aberrant restriction endonuclease digestion patterns of viral DNA. In the process of screening a large number of clinical adenovirus isolates by restriction endonuclease digestion of viral DNA, we have identified nine isolates from eight patients which were identified by neutralization as adenovirus type 1 or 2 but had aberrant cleavage patterns. Cleavage sites for the enzymes SmaI, EcoRI, HindIII, KpnI, and HpaI were mapped. The variants could be placed in six distinct groups based on cleavage patterns. The designation genome types 1a, 1b, 1c, 2b, 2c, and 2d are proposed for these isolates.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Aird F., King J. J., Younghusband H. B. Identification of a new strain of adenovirus type 2 by restriction endonuclease analysis. Gene. 1983 Apr;22(1):133–134. doi: 10.1016/0378-1119(83)90072-0. [DOI] [PubMed] [Google Scholar]
- Brůcková M., Wadell G., Sundell G., Syrůcek L., Kunzová L. An outbreak of respiratory disease due to a type 5 adenovirus identified as genome type 5a. Acta Virol. 1980 May;24(3):161–165. [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- Peacock A. C., Dingman C. W. Resolution of multiple ribonucleic acid species by polyacrylamide gel electrophoresis. Biochemistry. 1967 Jun;6(6):1818–1827. doi: 10.1021/bi00858a033. [DOI] [PubMed] [Google Scholar]
- Sambrook J., Sleigh M., Engler J. A., Broker T. R. The evolution of the adenoviral genome. Ann N Y Acad Sci. 1980;354:426–452. doi: 10.1111/j.1749-6632.1980.tb27983.x. [DOI] [PubMed] [Google Scholar]
- Siegel S. E., Patch C. T., Lewis A. M., Jr, Levine A. S. Simian virus 40 DNA replication, transcription, and antigen induction during infection with two adenovirus 2-SV40 hybrids that contain the entire SV40 genome. J Virol. 1975 Jul;16(1):43–52. doi: 10.1128/jvi.16.1.43-52.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Straube R. C., Thompson M. A., Van Dyke R. B., Wadell G., Connor J. D., Wingard D., Spector S. A. Adenovirus type 7b in a children's hospital. J Infect Dis. 1983 May;147(5):814–819. doi: 10.1093/infdis/147.5.814. [DOI] [PubMed] [Google Scholar]
- Wadell G., Hammarskjöld M. L., Winberg G., Varsanyi T. M., Sundell G. Genetic variability of adenoviruses. Ann N Y Acad Sci. 1980;354:16–42. doi: 10.1111/j.1749-6632.1980.tb27955.x. [DOI] [PubMed] [Google Scholar]
- Wadell G. Molecular epidemiology of human adenoviruses. Curr Top Microbiol Immunol. 1984;110:191–220. doi: 10.1007/978-3-642-46494-2_7. [DOI] [PubMed] [Google Scholar]
- Wadell G., de Jong J. C. Restriction endonucleases in identification of a genome type of adenovirus 19 associated with keratoconjunctivitis. Infect Immun. 1980 Feb;27(2):292–296. doi: 10.1128/iai.27.2.292-296.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]