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. 1985 Jan;21(1):24–28. doi: 10.1128/jcm.21.1.24-28.1985

Bacterial chromosomal restriction endonuclease analysis of the homology of Bacteroides species.

W C Bradbury, R G Murray, C Mancini, V L Morris
PMCID: PMC271573  PMID: 2981900

Abstract

Chromosomal DNAs of selected Bacteroides organisms whose relatedness had been previously determined by "conventional" filter-annealing studies (J. L. Johnson, Int. J. Syst. Bacteriol. 28:245, 1978) were further analyzed by restriction endonuclease analysis coupled with the Southern hybridization procedure (E. M. Southern, J. Mol. Biol. 98:503, 1975). By comparing their EcoRI restriction fragment patterns in agarose gel electrophoresis, each Bacteroides strain could be clearly differentiated. As a simple and direct means for comparison purposes this method was particularly useful for differentiating genetically similar organisms such as Bacteroides strains of the same species which shared greater than 75% homology. In contrast, bacterial chromosomal restriction endonuclease analysis in conjunction with Southern hybridizations was most effectively used to determine the significance of low levels of homology (less than 24%) as this technique provided additional information on the nature and relative distribution of that homology when the areas of homology were displayed as reproducible bands in autoradiograms.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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