Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Jan;21(1):97–101. doi: 10.1128/jcm.21.1.97-101.1985

Demonstration of a common antigen in sonicated cells for identification of Vibrio vulnificus serotypes.

M Nishibuchi, R J Seidler
PMCID: PMC271583  PMID: 3918076

Abstract

Ouchterlony immunodiffusion of sonicated Vibrio vulnificus cells illustrated a single major precipitation line with antiserum prepared from whole cells of the same species. Antigenic analysis by two-dimensional immunoelectrophoresis verified the presence of a single dominant precipitation line. Tandem two-dimensional immunoelectrophoretic analyses of V. vulnificus antigens from various strains revealed one fused precipitation line of identity. No fused precipitation lines were seen with other Vibrio species tested. This dominant antigen, designated VVA, was not dialyzable, lost antigenicity by heating at 100 degrees C but not at 70 degrees C, and was precipitated by 70%, but not by 50%, saturated ammonium sulfate. VVA was not present in concentrated (20X) spent culture medium. VVA is possibly an intracellular protein specific to V. vulnificus species and may be useful in the serological identification of this important human pathogen.

Full text

PDF
97

Images in this article

98Image
on p.98
99Image
on p.99
100Image
on p.100

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake P. A., Merson M. H., Weaver R. E., Hollis D. G., Heublein P. C. Disease caused by a marine Vibrio. Clinical characteristics and epidemiology. N Engl J Med. 1979 Jan 4;300(1):1–5. doi: 10.1056/NEJM197901043000101. [DOI] [PubMed] [Google Scholar]
  2. Blake P. A., Weaver R. E., Hollis D. G. Diseases of humans (other than cholera) caused by vibrios. Annu Rev Microbiol. 1980;34:341–367. doi: 10.1146/annurev.mi.34.100180.002013. [DOI] [PubMed] [Google Scholar]
  3. Bonner J. R., Coker A. S., Berryman C. R., Pollock H. M. Spectrum of Vibrio infections in a Gulf Coast community. Ann Intern Med. 1983 Oct;99(4):464–469. doi: 10.7326/0003-4819-99-4-464. [DOI] [PubMed] [Google Scholar]
  4. Farmer J. J., 3rd Vibrio ("Beneckea") vulnificus, the bacterium associated with sepsis, septicaemia, and the sea. Lancet. 1979 Oct 27;2(8148):903–903. doi: 10.1016/s0140-6736(79)92715-6. [DOI] [PubMed] [Google Scholar]
  5. Hollis D. G., Weaver R. E., Baker C. N., Thornsberry C. Halophilic Vibrio species isolated from blood cultures. J Clin Microbiol. 1976 Apr;3(4):425–431. doi: 10.1128/jcm.3.4.425-431.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kelly M. T., Avery D. M. Lactose-positive Vibrio in seawater: a cause of pneumonia and septicemia in a drowning victim. J Clin Microbiol. 1980 Mar;11(3):278–280. doi: 10.1128/jcm.11.3.278-280.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nishibuchi M., Seidler R. J. Rapid microimmunodiffusion method with species-specific antiserum raised to purified antigen for identification of Vibrio vulnificus. J Clin Microbiol. 1985 Jan;21(1):102–107. doi: 10.1128/jcm.21.1.102-107.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Reichelt J. L., Baumann P., Baumann L. Study of genetic relationships among marine species of the genera Beneckea and Photobacterium by means of in vitro DNA/DNA hybridization. Arch Microbiol. 1976 Oct 11;110(1):101–120. doi: 10.1007/BF00416975. [DOI] [PubMed] [Google Scholar]
  9. Roland F. P. Leg gangrene and endotoxin shock due to vibrio parahaemolyticus--an infection acquired in New England coastal waters. N Engl J Med. 1970 Jun 4;282(23):1306–1306. doi: 10.1056/NEJM197006042822306. [DOI] [PubMed] [Google Scholar]
  10. Thorsteinsson S. B., Minuth J. N., Musher D. M. Clinical manifestations of halophilic non-cholera Vibrio infections. Lancet. 1974 Nov 30;2(7892):1283–1284. doi: 10.1016/s0140-6736(74)90141-x. [DOI] [PubMed] [Google Scholar]
  11. Tison D. L., Nishibuchi M., Greenwood J. D., Seidler R. J. Vibrio vulnificus biogroup 2: new biogroup pathogenic for eels. Appl Environ Microbiol. 1982 Sep;44(3):640–646. doi: 10.1128/aem.44.3.640-646.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weaver R. E., Ehrenkranz N. J. Letter: Vibrio parahaemolyticus septicemia. Arch Intern Med. 1975 Jan;135(1):197–197. doi: 10.1001/archinte.135.1.197. [DOI] [PubMed] [Google Scholar]
  13. Zide N., Davis J., Ehrenkranz N. J. Fulminating vibrio parahemolyticus septicemia. A syndrome of erythemia multiforme, hemolytic anemia, and hypotension. Arch Intern Med. 1974 Mar;133(3):479–481. doi: 10.1001/archinte.133.3.479. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES