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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Feb;21(2):264–266. doi: 10.1128/jcm.21.2.264-266.1985

Isolation and enumeration of Clostridium botulinum by direct inoculation of infant fecal specimens on egg yolk agar and Clostridium botulinum isolation media.

C Glasby, C L Hatheway
PMCID: PMC271630  PMID: 3882751

Abstract

Direct plating of stool specimens on selective (Clostridium botulinum isolation) and nonselective (egg yolk agar) media was evaluated as an aid in confirming infant botulism. C. botulinum was isolated from 13 of 14 culture-positive specimens with C. botulinum isolation and from 8 of 14 egg yolk agar. No lipase reaction was seen on plates of 31 culture-negative specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Dezfulian M., Dowell V. R., Jr Cultural and physiological characteristics and antimicrobial susceptibility of Clostridium botulinum isolates from foodborne and infant botulism cases. J Clin Microbiol. 1980 Jun;11(6):604–609. doi: 10.1128/jcm.11.6.604-609.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Dezfulian M., McCroskey L. M., Hatheway C. L., Dowell V. R., Jr Selective medium for isolation of Clostridium botulinum from human feces. J Clin Microbiol. 1981 Mar;13(3):526–531. doi: 10.1128/jcm.13.3.526-531.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dowell V. R., Jr, McCroskey L. M., Hatheway C. L., Lombard G. L., Hughes J. M., Merson M. H. Coproexamination for botulinal toxin and clostridium botulinum. A new procedure for laboratory diagnosis of botulism. JAMA. 1977 Oct 24;238(17):1829–1832. [PubMed] [Google Scholar]
  4. Hatheway C. L. Laboratory procedures for cases of suspected infant botulism. Rev Infect Dis. 1979 Jul-Aug;1(4):647–651. doi: 10.1093/clinids/1.4.647. [DOI] [PubMed] [Google Scholar]
  5. Midura T. F., Arnon S. S. Infant botulism. Identification of Clostridium botulinum and its toxins in faeces. Lancet. 1976 Oct 30;2(7992):934–936. doi: 10.1016/s0140-6736(76)90894-1. [DOI] [PubMed] [Google Scholar]

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