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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Feb;21(2):286–287. doi: 10.1128/jcm.21.2.286-287.1985

Monoclonal antibodies against respiratory syncytial virus and their use for rapid detection of virus in nasopharyngeal secretions.

P Pothier, J C Nicolas, G P de Saint Maur, S Ghim, A Kazmierczak, F Bricout
PMCID: PMC271639  PMID: 3882752

Abstract

We developed five monoclonal antibodies against respiratory syncytial virus. Three of these (23A3, 12A4, and 18B2) were used in an indirect fluorescent antibody test, and the results were compared with those of a similar indirect fluorescent test with commercial anti-respiratory syncytial virus serum. The results obtained with antibody 18B2 and commercial anti-respiratory syncytial virus serum were identical, whereas with antibodies 23A3 and 12A4 the incidence of positive identifications was around 50%.

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Selected References

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  1. Bell D. M., Walsh E. E., Hruska J. F., Schnabel K. C., Hall C. B. Rapid detection of respiratory syncytial virus with a monoclonal antibody. J Clin Microbiol. 1983 Jun;17(6):1099–1101. doi: 10.1128/jcm.17.6.1099-1101.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Freymuth F. Rapid diagnosis of respiratory syncytial virus infections in children. Lancet. 1980 Sep 6;2(8193):539–540. doi: 10.1016/s0140-6736(80)91866-8. [DOI] [PubMed] [Google Scholar]
  3. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  4. Gardner P. S., McQuillin J., McGuckin R. The late detection of respiratory syncytial virus in cells of respiratory tract by immunofluorescence. J Hyg (Lond) 1970 Dec;68(4):575–580. doi: 10.1017/s0022172400042509. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gimenez H. B., Cash P., Melvin W. T. Monoclonal antibodies to human respiratory syncytial virus and their use in comparison of different virus isolates. J Gen Virol. 1984 May;65(Pt 5):963–971. doi: 10.1099/0022-1317-65-5-963. [DOI] [PubMed] [Google Scholar]
  6. Hall C. B., McBride J. T., Walsh E. E., Bell D. M., Gala C. L., Hildreth S., Ten Eyck L. G., Hall W. J. Aerosolized ribavirin treatment of infants with respiratory syncytial viral infection. A randomized double-blind study. N Engl J Med. 1983 Jun 16;308(24):1443–1447. doi: 10.1056/NEJM198306163082403. [DOI] [PubMed] [Google Scholar]
  7. Hall C. B. Nosocomial viral respiratory infections: perennial weeds on pediatric wards. Am J Med. 1981 Mar;70(3):670–676. doi: 10.1016/0002-9343(81)90594-5. [DOI] [PubMed] [Google Scholar]
  8. Kaul A., Scott R., Gallagher M., Scott M., Clement J., Ogra P. L. Respiratory syncytial virus infection. Rapid diagnosis in children by use of indirect immunofluorescence. Am J Dis Child. 1978 Nov;132(11):1088–1090. doi: 10.1001/archpedi.1978.02120360044006. [DOI] [PubMed] [Google Scholar]
  9. Kim H. W., Wyatt R. G., Fernie B. F., Brandt C. D., Arrobio J. O., Jeffries B. C., Parrott R. H. Respiratory syncytial virus detection by immunofluorescence in nasal secretions with monoclonal antibodies against selected surface and internal proteins. J Clin Microbiol. 1983 Dec;18(6):1399–1404. doi: 10.1128/jcm.18.6.1399-1404.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  11. Lauer B. A. Comparison of virus culturing and immunofluorescence for rapid detection of respiratory syncytial virus in nasopharyngeal secretions: sensitivity and specificity. J Clin Microbiol. 1982 Aug;16(2):411–412. doi: 10.1128/jcm.16.2.411-412.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McIntosh K., Hendry R. M., Fahnestock M. L., Pierik L. T. Enzyme-linked immunosorbent assay for detection of respiratory syncytial virus infection: application to clinical samples. J Clin Microbiol. 1982 Aug;16(2):329–333. doi: 10.1128/jcm.16.2.329-333.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McQuillin J., Gardner P. S. Rapid diagnosis of respiratory syncytial virus infection by immunofluorescent antibody techniques. Br Med J. 1968 Mar 9;1(5592):602–605. doi: 10.1136/bmj.1.5592.602. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Minnich L., Ray C. G. Comparison of direct immunofluorescent staining of clinical specimens for respiratory virus antigens with conventional isolation techniques. J Clin Microbiol. 1980 Sep;12(3):391–394. doi: 10.1128/jcm.12.3.391-394.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Orstavik I., Carlsen K. H., Halvorsen K. Respiratory syncytial virus infections in Oslo 1972--1978. I. Virological and epidemiological studies. Acta Paediatr Scand. 1980 Nov;69(6):717–722. doi: 10.1111/j.1651-2227.1980.tb07139.x. [DOI] [PubMed] [Google Scholar]

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