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. 1985 Mar;21(3):307–309. doi: 10.1128/jcm.21.3.307-309.1985

Improved recovery of group A beta-hemolytic streptococci with a new selective medium.

J R Carlson, W G Merz, B E Hansen, S Ruth, D G Moore
PMCID: PMC271652  PMID: 3884651

Abstract

A new selective group A streptococcus agar (ssA) (BBL Microbiology Systems, Cockeysville, Md.) was evaluated for the recovery and direct plate identification of group A beta-hemolytic streptococci (GABHS) in comparison with sheep blood agar (SBA). A total of 265 GABHS were recovered from 1,116 throat swab specimens on both media, with detection rates of 98.9 and 91.7% for ssA and SBA, respectively. Primary bacitracin disk susceptibility tests were performed on 549 specimens, and presumptive identifications were possible for 81.4% of the GABHS on ssA, as compared with 44.2% on SBA. All of the 120 GABHS recovered from another 567 specimens were identified by a coagglutination method after overnight incubation on ssA, whereas only 70.2% were identified at this interval on SBA. The major advantage of the ssA was the inhibition of normal respiratory flora, permitting improved recovery and rapid identification of GABHS.

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Selected References

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  1. Black W. A., Van Buskirk F. Gentamicin as a selective agent for the isolation of beta haemolytic streptococci. J Clin Pathol. 1973 Feb;26(2):154–156. doi: 10.1136/jcp.26.2.154. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Christensen P., Kahlmeter G., Jonsson S., Kronvall G. New method for the serological grouping of Streptococci with specific antibodies adsorbed to protein A-containing staphylococci. Infect Immun. 1973 Jun;7(6):881–885. doi: 10.1128/iai.7.6.881-885.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dykstra M. A., McLaughlin J. C., Bartlett R. C. Comparison of media and techniques for detection of group A streptococci in throat swab specimens. J Clin Microbiol. 1979 Feb;9(2):236–238. doi: 10.1128/jcm.9.2.236-238.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Facklam R. R., Padula J. F., Thacker L. G., Wortham E. C., Sconyers B. J. Presumptive identification of group A, B, and D streptococci. Appl Microbiol. 1974 Jan;27(1):107–113. doi: 10.1128/am.27.1.107-113.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gunn B. A., Ohashi D. K., Gaydos C. A., Holt E. S. Selective and enhanced recovery of group A and B streptococci from throat cultures with sheep blood agar containing sulfamethoxazole and trimethoprim. J Clin Microbiol. 1977 Jun;5(6):650–655. doi: 10.1128/jcm.5.6.650-655.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kurzynski T. A., Meise C. K. Evaluation of sulfamethoxazole-trimethoprim blood agar plates for recovery of group A streptocci from throat cultures. J Clin Microbiol. 1979 Feb;9(2):189–193. doi: 10.1128/jcm.9.2.189-193.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kurzynski T., Meise C., Daggs R., Helstad A. Improved reliability of the primary plate bacitracin test on throat cultures with sulfamethoxazole-trimethoprim blood agar plates. J Clin Microbiol. 1979 Jan;9(1):144–146. doi: 10.1128/jcm.9.1.144-146.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Libertin C. R., Wold A. D., Washington J. A., 2nd Effects of trimethoprim-sulfamethoxazole and incubation atmosphere on isolation of group A streptococci. J Clin Microbiol. 1983 Sep;18(3):680–682. doi: 10.1128/jcm.18.3.680-682.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Murray P. R., Wold A. D., Schreck C. A., Washington JA I. I. Effects of selective media and atmosphere of incubation on the isolation of group A streptococci. J Clin Microbiol. 1976 Jul;4(1):54–56. doi: 10.1128/jcm.4.1.54-56.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Sanders E. Bacterial interference. I. Its occurrence among the respiratory tract flora and characterization of inhibition of group A streptococci by viridans streptococci. J Infect Dis. 1969 Dec;120(6):698–707. doi: 10.1093/infdis/120.6.698. [DOI] [PubMed] [Google Scholar]
  11. Vincent W. F., Gibbons W. E., Gaafar H. A. Selective medium for the isolation of streptococci from clinical specimens. Appl Microbiol. 1971 Nov;22(5):942–943. doi: 10.1128/am.22.5.942-943.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]

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