Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Mar;21(3):457–458. doi: 10.1128/jcm.21.3.457-458.1985

Evaluation of a commercial enzyme-linked immunosorbent assay for the detection of herpes simplex virus.

D L Sewell, S A Horn
PMCID: PMC271687  PMID: 2984248

Abstract

A total of 136 specimens were tested for the presence of herpes simplex virus by routine tissue culture and a commercial enzyme-linked immunosorbent assay (Ortho Diagnostic Systems, Inc.). Forty-six (33.8%) of the specimens were positive by tissue culture. The sensitivity and specificity of the commercial system were 69.6 and 93.3%, respectively. The commercial system was rapid and moderately specific but lacked the sensitivity necessary for direct specimen testing.

Full text

PDF
457

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Corey L., Adams H. G., Brown Z. A., Holmes K. K. Genital herpes simplex virus infections: clinical manifestations, course, and complications. Ann Intern Med. 1983 Jun;98(6):958–972. doi: 10.7326/0003-4819-98-6-958. [DOI] [PubMed] [Google Scholar]
  2. Fayram S. L., Aarnaes S., de la Maza L. M. Comparison of cultureset to a conventional tissue culture-fluorescent-antibody technique for isolation and identification of herpes simplex virus. J Clin Microbiol. 1983 Jul;18(1):215–216. doi: 10.1128/jcm.18.1.215-216.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kibrick S. Herpes simplex infection at term. What to do with mother, newborn, and nursery personnel. JAMA. 1980 Jan 11;243(2):157–160. doi: 10.1001/jama.243.2.157. [DOI] [PubMed] [Google Scholar]
  4. Land S. A., Skurrie I. J., Gilbert G. L. Rapid diagnosis of herpes simplex virus infections by enzyme-linked immunosorbent assay. J Clin Microbiol. 1984 Jun;19(6):865–869. doi: 10.1128/jcm.19.6.865-869.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Morgan M. A., Smith T. F. Evaluation of an enzyme-linked immunosorbent assay for the detection of herpes simplex virus antigen. J Clin Microbiol. 1984 Jun;19(6):730–732. doi: 10.1128/jcm.19.6.730-732.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Sewell D. L., Horn S. A., Dilbeck P. W. Comparison of Cultureset and Bartels Immunodiagnostics with conventional tissue culture for isolation and identification of herpes simplex virus. J Clin Microbiol. 1984 May;19(5):705–706. doi: 10.1128/jcm.19.5.705-706.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Warford A. L., Levy R. A., Rekrut K. A. Evaluation of a commercial enzyme-linked immunosorbent assay for detection of herpes simplex virus antigen. J Clin Microbiol. 1984 Sep;20(3):490–493. doi: 10.1128/jcm.20.3.490-493.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Whitley R. J., Nahmias A. J., Soong S. J., Galasso G. G., Fleming C. L., Alford C. A. Vidarabine therapy of neonatal herpes simplex virus infection. Pediatrics. 1980 Oct;66(4):495–501. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES