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(a) Distribution of the time between red and green fluorescent bursts at PCR cycle 0, corresponding to the detection of uncorrelated PCR primers. (b) Distribution of the time between red and green fluorescent bursts at PCR cycle 40. A large central peak is observed, corresponding to the coincident detection of red and green fluorescent bursts, resulting from PCR amplicons incorporating both primers. The bin time is 2 ms.
