Abstract
Standardized, evenly dispersed hyphal suspensions served as the inoculum in a microtiter technique for amphotericin B antifungal susceptibility testing. Preliminary testing with six strains of Aspergillus fumigatus and A. flavus produced consistent and reproducible results at 30 degrees C over 24 h. The observed amphotericin B MICs required for hyphae (0.3 to 0.6 microgram/ml) were comparable to MICs required for conidia (0.16 to 0.6 microgram/ml). The results were evaluated and compared with previously published information.
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Selected References
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- Brandsberg J. W., French M. E. In vitro susceptibility of isolates of Aspergillus fumigatus and Sporothrix schenckii to amphotericin B. Antimicrob Agents Chemother. 1972 Nov;2(5):402–404. doi: 10.1128/aac.2.5.402. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brinkmann F. Sekundäre Sensibilitätsänderung von Candida albicans gegen 5 Fluoro-cytosin (5 FC) in vitro. Vergleich von fünf Methoden im Hinblick auf Routineuntersuchungen. Z Hautkr. 1981 Oct 1;56(19):1255–1263. [PubMed] [Google Scholar]
- Carrizosa J., Levison M. E., Lawrence T., Kaye D. Cure of Aspergillus ustus endocarditis on a prosthetic valve. Arch Intern Med. 1974 Mar;133(3):486–490. [PubMed] [Google Scholar]
- Edwards J. E., Jr, Morrison J., Henderson D. K., Montgomerie J. Z. Combined effect of amphotericin B and rifampin on Candida species. Antimicrob Agents Chemother. 1980 Mar;17(3):484–487. doi: 10.1128/aac.17.3.484. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ellis N. S., Bartlett M. S., Smith J. W. Assay for yeast susceptibility to 5-fluorocytosine and amphotericin B in a frozen microtiter system. Am J Clin Pathol. 1979 Aug;72(2):194–198. doi: 10.1093/ajcp/72.2.194. [DOI] [PubMed] [Google Scholar]
- Fisher B. D., Armstrong D. Rapid microdilution-colorimetric assay for yeast susceptibility to fluorocytosine. Antimicrob Agents Chemother. 1977 Nov;12(5):614–617. doi: 10.1128/aac.12.5.614. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Granade T. C., Artis W. M. Antimycotic susceptibility testing of dermatophytes in microcultures with a standardized fragmented mycelial inoculum. Antimicrob Agents Chemother. 1980 Apr;17(4):725–729. doi: 10.1128/aac.17.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holt R. J. Laboratory tests of antifungal drugs. J Clin Pathol. 1975 Oct;28(10):767–774. doi: 10.1136/jcp.28.10.767. [DOI] [PMC free article] [PubMed] [Google Scholar]
- IKEMOTO H. TREATMENT OF PULMONARY ASPERGILLOMA WITH AMPHOTERICIN B. Arch Intern Med. 1965 May;115:598–601. doi: 10.1001/archinte.1960.03860170080017. [DOI] [PubMed] [Google Scholar]
- Kitahara M., Seth V. K., Medoff G., Kobayashi G. S. Antimicrobial susceptibility testing of six clinical isolates of Aspergillus. Antimicrob Agents Chemother. 1976 Jun;9(6):908–914. doi: 10.1128/aac.9.6.908. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Marks M. I., Eickhoff T. C. Application of four methods to the study of the susceptibility of yeast to 5-fluorocytosine. Antimicrob Agents Chemother (Bethesda) 1970;10:491–493. [PubMed] [Google Scholar]
- Mazens M. F., Andrews G. P., Bartlett R. C. Comparison of microdilution and broth dilution techniques for the susceptibility testing of yeasts to 5-fluorocytosine and amphotericin B. Antimicrob Agents Chemother. 1979 Mar;15(3):475–477. doi: 10.1128/aac.15.3.475. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Merz W. G., Fay D., Thumar B., Dixon D. Susceptibility testing of filamentous fungi to amphotericin B by a rapid radiometric method. J Clin Microbiol. 1984 Jan;19(1):54–56. doi: 10.1128/jcm.19.1.54-56.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Perfect J. R., Durack D. T. Treatment of experimental cryptococcal meningitis with amphotericin B, 5-fluorocytosine, and ketoconazole. J Infect Dis. 1982 Sep;146(3):429–435. doi: 10.1093/infdis/146.3.429. [DOI] [PubMed] [Google Scholar]
- Sandhu D. K., Doodani A., Singh D. Fungi associated with human corneal ulcers. Mykosen. 1980 May;23(5):269–273. [PubMed] [Google Scholar]
- Utz C. J., White S., Shadomy S. New medium for in vitro susceptibility studies with amphotericin B. Antimicrob Agents Chemother. 1976 Oct;10(4):776–777. doi: 10.1128/aac.10.4.776. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wagner G., Shadomy S., Paxton L. D., Espinel-Ingroff A. New method for susceptibility testing with antifungal agents. Antimicrob Agents Chemother. 1975 Jul;8(1):107–109. doi: 10.1128/aac.8.1.107. [DOI] [PMC free article] [PubMed] [Google Scholar]
