Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Apr;21(4):578–581. doi: 10.1128/jcm.21.4.578-581.1985

Detection of bacteriuria and pyuria within two minutes.

M T Pezzlo, M A Wetkowski, E M Peterson, L M de la Maza
PMCID: PMC271723  PMID: 3886693

Abstract

A study was performed to evaluate two rapid urine screening methods, Bac-T-Screen (Marion Laboratories, Inc., Kansas City, Mo.) and Chemstrip LN (Boehringer Mannheim Diagnostics, BioDynamics, Indianapolis, Ind.), for their ability to screen for bacteriuria and pyuria within 2 min. A total of 1,000 urine specimens were tested with the Bac-T-Screen and the Chemstrip LN and compared with a semiquantitative plate culture method. Of the 1,000 specimens tested, 249 had colony counts of greater than or equal to 10(5) CFU/ml by the culture method. Of these, the Bac-T-Screen detected 94.8% (236 of 249) and the Chemstrip LN detected 84.7% (210 of 249). There were 120 pure cultures of probable pathogens of which the Bac-T-Screen detected 97.5% (117 of 120) and the Chemstrip LN detected 91.7% (110 of 120). Leukocyte counts were performed on all specimens, and both methods have the ability to detect greater than 10 leukocytes per mm3 in a majority (greater than 93%) of the specimens. The cost per test for a negative screen is approximately $1.30 for the Bac-T-Screen and $0.40 for the Chemstrip LN. Overall there is a similar negative predictive value with both methods for bacteriuria and pyuria.

Full text

PDF
578

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Banauch D. Leukozyten-Nachweis im Urin mit einem Teststreifen. Eine kooperative Studie an elf Zentren. Dtsch Med Wochenschr. 1979 Aug 31;104(35):1236–1240. [PubMed] [Google Scholar]
  2. Davis J. R., Stager C. E., Araj G. F. Clinical laboratory evaluation of a bacteriuria detection device for urine screening. Am J Clin Pathol. 1984 Jan;81(1):48–53. doi: 10.1093/ajcp/81.1.48. [DOI] [PubMed] [Google Scholar]
  3. Gillenwater J. Y. Detection of urinary leukocytes by chemstrip-l. J Urol. 1981 Mar;125(3):383–384. doi: 10.1016/s0022-5347(17)55043-0. [DOI] [PubMed] [Google Scholar]
  4. Kusumi R. K., Grover P. J., Kunin C. M. Rapid detection of pyuria by leukocyte esterase activity. JAMA. 1981 Apr 24;245(16):1653–1655. [PubMed] [Google Scholar]
  5. Musher D. M., Thorsteinsson S. B., Airola VM I. I. Quantitative urinalysis. Diagnosing urinary tract infection in men. JAMA. 1976 Nov 1;236(18):2069–2072. doi: 10.1001/jama.236.18.2069. [DOI] [PubMed] [Google Scholar]
  6. Perry J. L., Matthews J. S., Weesner D. E. Evaluation of leukocyte esterase activity as a rapid screening technique for bacteriuria. J Clin Microbiol. 1982 May;15(5):852–854. doi: 10.1128/jcm.15.5.852-854.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Pezzlo M. T. Automated methods for detection of bacteriuria. Am J Med. 1983 Jul 28;75(1B):71–78. doi: 10.1016/0002-9343(83)90075-x. [DOI] [PubMed] [Google Scholar]
  8. Pezzlo M. T., Wetkowski M. A., Peterson E. M., de la Maza L. M. Evaluation of a two-minute test for urine screening. J Clin Microbiol. 1983 Sep;18(3):697–701. doi: 10.1128/jcm.18.3.697-701.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pfaller M. A., Baum C. A., Niles A. C., Murray P. R. Clinical laboratory evaluation of a urine screening device. J Clin Microbiol. 1983 Sep;18(3):674–679. doi: 10.1128/jcm.18.3.674-679.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Ransohoff D. F., Feinstein A. R. Problems of spectrum and bias in evaluating the efficacy of diagnostic tests. N Engl J Med. 1978 Oct 26;299(17):926–930. doi: 10.1056/NEJM197810262991705. [DOI] [PubMed] [Google Scholar]
  11. Smalley D. L., Dittmann A. N. Use of leukocyte esterase-nitrate activity as predictive assays of significant bacteriuria. J Clin Microbiol. 1983 Nov;18(5):1256–1257. doi: 10.1128/jcm.18.5.1256-1257.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Stamm W. E. Measurement of pyuria and its relation to bacteriuria. Am J Med. 1983 Jul 28;75(1B):53–58. doi: 10.1016/0002-9343(83)90073-6. [DOI] [PubMed] [Google Scholar]
  13. Stamm W. E. Recent developments in the diagnosis and treatment of urinary tract infections. West J Med. 1982 Sep;137(3):213–220. [PMC free article] [PubMed] [Google Scholar]
  14. Stamm W. E., Wagner K. F., Amsel R., Alexander E. R., Turck M., Counts G. W., Holmes K. K. Causes of the acute urethral syndrome in women. N Engl J Med. 1980 Aug 21;303(8):409–415. doi: 10.1056/NEJM198008213030801. [DOI] [PubMed] [Google Scholar]
  15. WILLIAMS J. D., LEIGH D. A., ROSSER E. I., BRUMFITT W., PATH M. C. THE ORGANIZATION AND RESULTS OF A SCREENING PROGRAMME FOR THE DETECTION OF BACTERIURIA OF PREGNANCY. J Obstet Gynaecol Br Commonw. 1965 Jun;72:327–335. doi: 10.1111/j.1471-0528.1965.tb01466.x. [DOI] [PubMed] [Google Scholar]
  16. Wallis C., Melnick J. L., Longoria C. J. Colorimetric method for rapid determination of bacteriuria. J Clin Microbiol. 1981 Sep;14(3):342–346. doi: 10.1128/jcm.14.3.342-346.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES