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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Apr;21(4):643–644. doi: 10.1128/jcm.21.4.643-644.1985

Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus.

E M Swierkosz, M Q Arens, R R Schmidt, T Armstrong
PMCID: PMC271739  PMID: 2985651

Abstract

An indirect immunofluorescence assay and a direct immunofluorescence assay were evaluated for typing clinical isolates of herpes simplex virus (HSV). The indirect immunofluorescence assay (Electro-Nucleonics, Inc.) correctly identified 16 HSV type 2 (HSV-2) isolates, but failed to identify 4 of 14 HSV-1 isolates because of background fluorescence and instability of reagents. Forty-nine HSV-1 isolates were correctly typed by direct immunofluorescence assay (Kallestad Laboratories, Inc.), but 1 of 39 HSV-2 isolates did not react with the HSV-2 type-specific antibody conjugate.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arens M. Q., Swierkosz E. M. Simplified method for typing herpes simplex virus by restriction endonuclease analysis. J Clin Microbiol. 1983 Mar;17(3):548–551. doi: 10.1128/jcm.17.3.548-551.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fife K. H., Fife R. S., Kleiman M. B., French M. L. Incorrect typing of herpes simplex virus isolates by direct immunofluorescence. J Infect Dis. 1983 Aug;148(2):338–338. doi: 10.1093/infdis/148.2.338. [DOI] [PubMed] [Google Scholar]
  3. McTaggart S. P., Burns W. H., White D. O., Jackson D. C. Fc receptors induced by herpes simplex virus. I. Biologic and biochemical properties. J Immunol. 1978 Aug;121(2):726–730. [PubMed] [Google Scholar]
  4. Pereira L., Dondero D. V., Gallo D., Devlin V., Woodie J. D. Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies. Infect Immun. 1982 Jan;35(1):363–367. doi: 10.1128/iai.35.1.363-367.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Zheng Z. M., Mayo D. R., Hsiung G. D. Comparison of biological, biochemical, immunological, and immunochemical techniques for typing herpes simplex virus isolates. J Clin Microbiol. 1983 Mar;17(3):396–399. doi: 10.1128/jcm.17.3.396-399.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]

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