Abstract
A coagglutination system has been devised for typing heat-stable and heat-labile antigens of Campylobacter jejuni and C. coli. The use of protein A-positive Staphylococcus aureus cells carrying Campylobacter sp. serotype antibody and the treatment of Campylobacter sp. cells with DNase in the antigen suspension permitted rapid and specific coagglutination of rough (autoagglutinable) as well as smooth cultures. Cells of S. aureus were sensitized with Campylobacter sp. serotype antisera. Four to five types of sensitized S. aureus cells were pooled. A strain of Campylobacter sp. was first tested with the pools and then typed with the individual reagents of the reactive pool. After the described procedures, 68 serotype strains tested blindly as unknowns were correctly typed according to their heat-stable or heat-labile antigens. The two most commonly used typing schemes which are based separately on the heat-stable or the heat-labile antigens as assayed by passive hemagglutination and slide agglutination, respectively, can be utilized simultaneously in the coagglutination system for strain characterization. The coagglutination system is simple, yields results rapidly, conserves typing reagents, and offers the flexibility of formulating the pools of reagents according to the experimental design or the prevalence of serotypes in a geographic location. It should be a practical system for the typing of Campylobacter spp. in public health or clinical laboratories.
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Selected References
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- Abbott J. D., Dale B., Eldridge J., Jones D. M., Sutcliffe E. M. Serotyping of Campylobacter jejuni/coli. J Clin Pathol. 1980 Aug;33(8):762–766. doi: 10.1136/jcp.33.8.762. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Arko R. J., Wong K. H., Peacock W. L. Nuclease enhancement of specific cell agglutination in a serodiagnostic test for Neisseria gonorrhoeae. J Clin Microbiol. 1979 Apr;9(4):517–519. doi: 10.1128/jcm.9.4.517-519.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Blaser M. J., Duncan D. J., Osterholm M. T., Istre G. R., Wang W. L. Serologic study of two clusters of infection due to Campylobacter jejuni. J Infect Dis. 1983 May;147(5):820–823. doi: 10.1093/infdis/147.5.820. [DOI] [PubMed] [Google Scholar]
- Kosunen T. U., Danielsson D., Kjellander J. Serology of Campylobacter fetus ss. jejuni )"related" campylobacters). Demonstration of strain-specific and interstrain-related antigens by immunoelectrophoresis and co-agglutination. Acta Pathol Microbiol Scand B. 1980 Aug;88(4):207–218. [PubMed] [Google Scholar]
- Lauwers S., Vlaes L., Butzler J. P. Campylobacter serotyping a epidemiology. Lancet. 1981 Jan 17;1(8212):158–159. doi: 10.1016/s0140-6736(81)90748-0. [DOI] [PubMed] [Google Scholar]
- Lior H., Woodward D. L., Edgar J. A., Laroche L. J., Gill P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol. 1982 May;15(5):761–768. doi: 10.1128/jcm.15.5.761-768.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McMyne P. M., Penner J. L., Mathias R. G., Black W. A., Hennessy J. N. Serotyping of Campylobacter jejuni isolated from sporadic cases and outbreaks in British Columbia. J Clin Microbiol. 1982 Aug;16(2):281–285. doi: 10.1128/jcm.16.2.281-285.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Penner J. L., Hennessy J. N., Congi R. V. Serotyping of Campylobacter jejuni and Campylobacter coli on the basis of thermostable antigens. Eur J Clin Microbiol. 1983 Aug;2(4):378–383. doi: 10.1007/BF02019474. [DOI] [PubMed] [Google Scholar]
- Penner J. L., Hennessy J. N. Passive hemagglutination technique for serotyping Campylobacter fetus subsp. jejuni on the basis of soluble heat-stable antigens. J Clin Microbiol. 1980 Dec;12(6):732–737. doi: 10.1128/jcm.12.6.732-737.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]