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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1985 Jun;21(6):899–901. doi: 10.1128/jcm.21.6.899-901.1985

Preparation of urine samples for use in commercial latex agglutination tests for bacterial antigens.

G A Weinberg, G A Storch
PMCID: PMC271814  PMID: 3874212

Abstract

The use of latex agglutination (LA) tests for bacterial antigen detection in urine specimens is hindered by troublesome reactions such as nonspecific agglutination. Therefore, procedures such as boiling or membrane filtration of urine specimens are often used before LA testing. We discovered that the composition of the membrane filter used in filtration has a marked effect on the performance of an LA test used for detection of Haemophilus influenzae type b antigen. False-positive LA reactivity was common in urine specimens from pediatric patients that were processed by membrane filtration through certain filters; furthermore, such reactivity also occurred in LA tests for antigens other than those of H. influenzae. A protein present in urine at low concentrations appeared to be responsible for these phenomena.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Coonrod J. D. Urine as an antigen reservoir for diagnosis of infectious diseases. Am J Med. 1983 Jul 28;75(1B):85–92. doi: 10.1016/0002-9343(83)90077-3. [DOI] [PubMed] [Google Scholar]
  2. Daum R. S., Siber G. R., Kamon J. S., Russell R. R. Evaluation of a commercial latex particle agglutination test for rapid diagnosis of Haemophilus influenzae type b infection. Pediatrics. 1982 Apr;69(4):466–471. [PubMed] [Google Scholar]
  3. Feigin R. D., Wong M., Shackelford P. G., Stechenberg B. W., Dunkle L. M., Kaplan S. Countercurrent immunoelectrophoresis of urine as well as of CSF and blood for diagnosis of bacterial meningitis. J Pediatr. 1976 Nov;89(5):773–775. doi: 10.1016/s0022-3476(76)80802-5. [DOI] [PubMed] [Google Scholar]
  4. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  5. Scheifele D. W., Daum R. S., Syriopoulou V. P., Siber G. R., Smith A. L. Comparison of two antigen detection techniques in a primate model of Haemophilus influenzae type b infection. Infect Immun. 1979 Dec;26(3):827–831. doi: 10.1128/iai.26.3.827-831.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Scheifele D. W., Ward J. I., Siber G. R. Advantage of latex agglutination over countercurrent immunoelectrophoresis in the detection of Haemophilus influenzae type b antigen in serum. Pediatrics. 1981 Dec;68(6):888–891. [PubMed] [Google Scholar]
  7. Shackelford P. G., Campbell J., Feigin R. D. Countercurrent immunoelectrophoresis in the evaluation of childhood infections. J Pediatr. 1974 Oct;85(4):478–481. doi: 10.1016/s0022-3476(74)80448-8. [DOI] [PubMed] [Google Scholar]

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