Abstract
Human cytomegalovirus induces the appearance of immediate early antigens in infected cells 1 h after infection. This provided the basis for the development of a rapid neutralization assay for cytomegalovirus antibody which was able to yield results within a single day. Indirect immunofluorescence to visualize immediate early antigen-positive cells was applied to the rapid determination of cytomegalovirus-neutralizing antibody. The neutralization titers obtained with this assay on 92 serum samples were in accordance with the immune status as determined by enzyme-linked immunosorbent assay to cytomegalovirus-induced immediate early, early, and late antigens.
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- Gerna G., Cereda P. M., Cattaneo E., Achilli G., Revello M. G. Immunoglobulin G to virus-specific early antigens in congenital, primary, and reactivated human cytomegalovirus infections. Infect Immun. 1978 Dec;22(3):833–841. doi: 10.1128/iai.22.3.833-841.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michelson-Fiske S., Horodniceanu F., Guillon J. C. Immediate early antigens in human cytomegalovirus infected cells. Nature. 1977 Dec 15;270(5638):615–617. doi: 10.1038/270615a0. [DOI] [PubMed] [Google Scholar]
- Musiani M., Carpi C., Zerbini M. Rapid detection of antibodies against cytomegalovirus induced immediate early and early antigens by an enzyme linked immunosorbent assay. J Clin Pathol. 1984 Feb;37(2):122–125. doi: 10.1136/jcp.37.2.122. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Musiani M., Zerbini M., Carpi C., Plazzi M., Sermasi G., Belletti D., Sacchi R. Serological screening for the prevention of transfusion-acquired cytomegalovirus infection. J Infect. 1984 Sep;9(2):148–152. doi: 10.1016/s0163-4453(84)91078-8. [DOI] [PubMed] [Google Scholar]
- Musiani M., Zerbini M. Influence of cell cycle on the efficiency of transfection with purified human cytomegalovirus DNA. Arch Virol. 1983;78(3-4):287–292. doi: 10.1007/BF01311323. [DOI] [PubMed] [Google Scholar]
- PLUMMER G., BENYESH-MELNICK M. A PLAQUE REDUCTION NEUTRALIZATION TEST FOR HUMAN CYTOMEGALOVIRUS. Proc Soc Exp Biol Med. 1964 Oct;117:145–150. doi: 10.3181/00379727-117-29520. [DOI] [PubMed] [Google Scholar]
- Pereira L., Hoffman M., Gallo D., Cremer N. Monoclonal antibodies to human cytomegalovirus: three surface membrane proteins with unique immunological and electrophoretic properties specify cross-reactive determinants. Infect Immun. 1982 Jun;36(3):924–932. doi: 10.1128/iai.36.3.924-932.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rundell B. B., Betts R. F. Physical properties of cytomegalorvirus immune complexes prepared with IgG neutralizing antibody, anti-IgG, and complement. J Immunol. 1980 Jan;124(1):337–342. [PubMed] [Google Scholar]
- Schmidt N. J., Dennis J., Lennette E. H. Plaque reduction neutralization test for human cytomegalovirus based upon enhanced uptake of neutral red by virus-infected cells. J Clin Microbiol. 1976 Jul;4(1):61–66. doi: 10.1128/jcm.4.1.61-66.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Stalder H., Ehrensberger A. Microneutalization of cytomegalovirus. J Infect Dis. 1980 Jul;142(1):102–105. doi: 10.1093/infdis/142.1.102. [DOI] [PubMed] [Google Scholar]
- Stinski M. F., Mocarski E. S., Thomsen D. R. DNA of human cytomegalovirus: size heterogeneity and defectiveness resulting from serial undiluted passage. J Virol. 1979 Jul;31(1):231–239. doi: 10.1128/jvi.31.1.231-239.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- The T. H., Andersen H. K., Spencer E. S., Klein G. Antibodies against cytomegalovirus-induced early antigens (CMV-EA) in immunosuppressed renal-allograft recipients. Clin Exp Immunol. 1977 Jun;28(3):502–505. [PMC free article] [PubMed] [Google Scholar]
- Waner J. L., Budnick J. E. Three-day assay for human cytomegalovirus applicable to serum neutralization tests. Appl Microbiol. 1973 Jan;25(1):37–39. doi: 10.1128/am.25.1.37-39.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zerbini M., Musiani M., Carpi C., Falcieri E. Productive and abortive replication of human cytomegalovirus at different environmental pH values. Arch Virol. 1982;72(1-2):127–131. doi: 10.1007/BF01314457. [DOI] [PubMed] [Google Scholar]