Abstract
A dot-blot hybridization test was developed which allowed the direct detection of fastidious enteric adenovirus DNA in stool specimens from children with diarrhea and simultaneous typing of the viruses as adenovirus type 40 (Ad40) or Ad41. Cloned PstI fragments of Ad40 and Ad41 were used as 32P-labeled probes in the test, which allowed detection of picogram quantities of viral DNA in 20 to 40 microliter of stool suspension. Results were obtained within 48 h. The type specificity of the test was evaluated with 76 specimens known to contain either Ad40 or Ad41 by restriction enzyme analysis. Sixty-one specimens had sufficient DNA to be detected without any removal of protein. Thirty-one adenoviruses were typed as Ad40, and 30 were typed as Ad41, giving 100% correlation with the results of restriction enzyme analysis. The other 15 specimens were detected and typed as Ad40 or Ad41 only after removal of protein by a phenol extraction method. The dot-blot hybridization method is particularly useful for identifying those Ad40 and Ad41 strains which defy all attempts at culture and will be a useful tool in the epidemiology of fastidious enteric adenovirus infections.
Full text
PDF





Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allard A. K., Wadell G., Evander K. M., Lindman G. K. Specific properties of two enteric adenovirus 41 clones mapped within early region 1A. J Virol. 1985 Apr;54(1):145–150. doi: 10.1128/jvi.54.1.145-150.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brandt C. D., Kim H. W., Rodriguez W. J., Arrobio J. O., Jeffries B. C., Stallings E. P., Lewis C., Miles A. J., Gardner M. K., Parrott R. H. Adenoviruses and pediatric gastroenteritis. J Infect Dis. 1985 Mar;151(3):437–443. doi: 10.1093/infdis/151.3.437. [DOI] [PubMed] [Google Scholar]
- Brandt C. D., Rodriguez W. J., Kim H. W., Arrobio J. O., Jeffries B. C., Parrott R. H. Rapid presumptive recognition of diarrhea-associated adenoviruses. J Clin Microbiol. 1984 Nov;20(5):1008–1009. doi: 10.1128/jcm.20.5.1008-1009.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Brown M., Petric M., Middleton P. J. Diagnosis of fastidious enteric adenoviruses 40 and 41 in stool specimens. J Clin Microbiol. 1984 Sep;20(3):334–338. doi: 10.1128/jcm.20.3.334-338.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Buitenwerf J., Louwerens J. J., De Jong J. C. A simple and rapid method for typing adenoviruses 40 and 41 without cultivation. J Virol Methods. 1985 Jan;10(1):39–44. doi: 10.1016/0166-0934(85)90086-2. [DOI] [PubMed] [Google Scholar]
- Chiba S., Nakata S., Nakamura I., Taniguchi K., Urasawa S., Fujinaga K., Nakao T. Outbreak of infantile gastroenteritis due to type 40 adenovirus. Lancet. 1983 Oct 22;2(8356):954–957. doi: 10.1016/s0140-6736(83)90463-4. [DOI] [PubMed] [Google Scholar]
- Hirt B. Selective extraction of polyoma DNA from infected mouse cell cultures. J Mol Biol. 1967 Jun 14;26(2):365–369. doi: 10.1016/0022-2836(67)90307-5. [DOI] [PubMed] [Google Scholar]
- Kidd A. H., Banatvala J. E., de Jong J. C. Antibodies to fastidious faecal adenoviruses (species 40 and 41) in sera from children. J Med Virol. 1983;11(4):333–341. doi: 10.1002/jmv.1890110409. [DOI] [PubMed] [Google Scholar]
- Kidd A. H., Berkowitz F. E., Blaskovic P. J., Schoub B. D. Genome variants of human adenovirus 40 (subgroup F). J Med Virol. 1984;14(3):235–246. doi: 10.1002/jmv.1890140307. [DOI] [PubMed] [Google Scholar]
- Kidd A. H., Cosgrove B. P., Brown R. A., Madeley C. R. Faecal adenoviruses from Glasgow babies. Studies on culture and identity. J Hyg (Lond) 1982 Jun;88(3):463–474. doi: 10.1017/s0022172400070327. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kidd A. H. Genome variants of adenovirus 41 (subgroup G) from children with diarrhoea in South Africa. J Med Virol. 1984;14(1):49–59. doi: 10.1002/jmv.1890140108. [DOI] [PubMed] [Google Scholar]
- Kidd A. H., Madeley C. R. In vitro growth of some fastidious adenoviruses from stool specimens. J Clin Pathol. 1981 Feb;34(2):213–216. doi: 10.1136/jcp.34.2.213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morris C. A., Flewett T. H., Bryden A. S., Davies H. Epidemic viral enteritis in a long-stay children's ward. Lancet. 1975 Jan 4;1(7897):4–5. doi: 10.1016/s0140-6736(75)92370-3. [DOI] [PubMed] [Google Scholar]
- Richmond S. J., Caul E. O., Dunn S. M., Ashley C. R., Clarke S. K., Seymour N. R. An outbreak of gastroenteritis in young children caused by adenoviruses. Lancet. 1979 Jun 2;1(8127):1178–1181. doi: 10.1016/s0140-6736(79)91853-1. [DOI] [PubMed] [Google Scholar]
- Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
- Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
- Takiff H. E., Reinhold W., Garon C. F., Straus S. E. Cloning and physical mapping of enteric adenoviruses (candidate types 40 and 41). J Virol. 1984 Jul;51(1):131–136. doi: 10.1128/jvi.51.1.131-136.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Takiff H. E., Straus S. E., Garon C. F. Propagation and in vitro studies of previously non-cultivable enteral adenoviruses in 293 cells. Lancet. 1981 Oct 17;2(8251):832–834. doi: 10.1016/s0140-6736(81)91104-1. [DOI] [PubMed] [Google Scholar]
- Uhnoo I., Wadell G., Svensson L., Johansson M. E. Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J Clin Microbiol. 1984 Sep;20(3):365–372. doi: 10.1128/jcm.20.3.365-372.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Uhnoo I., Wadell G., Svensson L., Johansson M. Two new serotypes of enteric adenovirus causing infantile diarrhoea. Dev Biol Stand. 1983;53:311–318. [PubMed] [Google Scholar]
- Wadell G. Molecular epidemiology of human adenoviruses. Curr Top Microbiol Immunol. 1984;110:191–220. doi: 10.1007/978-3-642-46494-2_7. [DOI] [PubMed] [Google Scholar]
- Whitelaw A., Davies H., Parry J. Eelectron microscopy of fatal adenovirus gastroenteritis. Lancet. 1977 Feb 12;1(8007):361–361. doi: 10.1016/s0140-6736(77)91158-8. [DOI] [PubMed] [Google Scholar]
- Yolken R. H., Lawrence F., Leister F., Takiff H. E., Strauss S. E. Gastroenteritis associated with enteric type adenovirus in hospitalized infants. J Pediatr. 1982 Jul;101(1):21–26. doi: 10.1016/s0022-3476(82)80173-x. [DOI] [PubMed] [Google Scholar]
- de Jong J. C., Wigand R., Kidd A. H., Wadell G., Kapsenberg J. G., Muzerie C. J., Wermenbol A. G., Firtzlaff R. G. Candidate adenoviruses 40 and 41: fastidious adenoviruses from human infant stool. J Med Virol. 1983;11(3):215–231. doi: 10.1002/jmv.1890110305. [DOI] [PubMed] [Google Scholar]
- van Loon A. E., Rozijn T. H., de Jong J. C., Sussenbach J. S. Physicochemical properties of the DNAs of the fastidious adenovirus species 40 and 41. Virology. 1985 Jan 15;140(1):197–200. doi: 10.1016/0042-6822(85)90461-1. [DOI] [PubMed] [Google Scholar]