Abstract
Two varieties of agar medium (Trypticase [BBL Microbiology Systems]-serum-sorbitol-bromcresol purple agar [TSSB] and Trypticase-blood-sorbitol-CaCO3 agar [TBSCa]) indicating microbial acid production from sorbitol were tested. The media were devised for use in studies on the prevalence of sorbitol-fermenting human oral microorganisms incubated in an anaerobic or microaerophilic atmosphere containing 5 to 6% CO2. TSSB contains bromcresol purple as the pH indicator and NaHCO3 as the main buffering salt. TBSCa contains CaCO3 as both the buffering salt and the indicator of acid production. The growth yield of pure cultures of oral microorganisms on TBSCa was shown to equal that on blood agar incubated under similar conditions. TSSB inhibited the growth of several bacteria to various extents. The recovery of sorbitol-fermenting microorganisms from oral specimens was the greatest when the specimens were assayed with TBSCa. The poorer results obtained with TSSB were mainly due to the decoloration of the pH indicator in this medium and the presence of greater numbers of sorbitol false-positive colonies.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Birkhed D., Edwardsson S., Ahldén M. L., Frostell G. Effects of 3 months frequent consumption of hydrogenated starch hydrolysate (Lycasin), maltitol, sorbitol and xylitol on human dental plaque. Acta Odontol Scand. 1979;37(2):103–115. doi: 10.3109/00016357909027577. [DOI] [PubMed] [Google Scholar]
- CHRISTENSEN H. E., HJORT G. H. Spleen-shielding in x-irradiation-acclerated experimental amyloidosis in mice. Acta Pathol Microbiol Scand. 1960;48:1–12. doi: 10.1111/j.1699-0463.1960.tb04729.x. [DOI] [PubMed] [Google Scholar]
- Cornick D. E., Bowen W. H. The effect of sorbitol on the microbiology of the dental plaque in monkeys (Macaca irus). Arch Oral Biol. 1972 Dec;17(12):1637–1648. doi: 10.1016/0003-9969(72)90226-9. [DOI] [PubMed] [Google Scholar]
- Handelman S. L., Mills J. R., Meggo L. A medium for differentiating acidogenic bacteria. Arch Oral Biol. 1968 Oct;13(10):1187–1196. doi: 10.1016/0003-9969(68)90074-5. [DOI] [PubMed] [Google Scholar]
- MORSE M. L., ALIRE M. L. An agar medium indicating acid production. J Bacteriol. 1958 Sep;76(3):270–271. doi: 10.1128/jb.76.3.270-271.1958. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rateitschak-Plüss E. M., Guggenheim B. Effects of a carbohydrate-free diet and sugar substitutes on dental plaque accumulation. J Clin Periodontol. 1982 May;9(3):239–251. doi: 10.1111/j.1600-051x.1982.tb02063.x. [DOI] [PubMed] [Google Scholar]
- Wade W. E., Smiley K. L., Boruff C. S. An Improved Method for Differentiating Acid-forming from Non-acid-forming Bacteria. J Bacteriol. 1946 Jun;51(6):787–788. [PMC free article] [PubMed] [Google Scholar]