Abstract
Virus infectivity and antibody neutralization titers for respiratory syncytial virus were determined in cell cultures in microtiter plates. After an appropriate incubation period, the cells were fixed, and an enzyme-linked immunosorbent assay was performed directly in the microtiter plates for detection of virus. Results could be read and recorded automatically, which is especially helpful when running large numbers of tests.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Anderson L. J., Hierholzer J. C., Tsou C., Hendry R. M., Fernie B. F., Stone Y., McIntosh K. Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. J Infect Dis. 1985 Apr;151(4):626–633. doi: 10.1093/infdis/151.4.626. [DOI] [PubMed] [Google Scholar]
- CHANOCK R., ROIZMAN B., MYERS R. Recovery from infants with respiratory illness of a virus related to chimpanzee coryza agent (CCA). I. Isolation, properties and characterization. Am J Hyg. 1957 Nov;66(3):281–290. doi: 10.1093/oxfordjournals.aje.a119901. [DOI] [PubMed] [Google Scholar]
- Gerna G., Cereda P. M., Revello M. G., Cattaneo E., Battaglia M., Gerna M. T. Antigenic and biological relationships between human coronavirus OC43 and neonatal calf diarrhoea coronavirus. J Gen Virol. 1981 May;54(Pt 1):91–102. doi: 10.1099/0022-1317-54-1-91. [DOI] [PubMed] [Google Scholar]
- Grom J., Bernard S. Virus enzyme-linked cell immunoassay (VELCIA): detection and titration of rotavirus antigen and demonstration of rotavirus neutralizing and total antibodies. J Virol Methods. 1985 Feb;10(2):135–144. doi: 10.1016/0166-0934(85)90099-0. [DOI] [PubMed] [Google Scholar]
- Lemon S. M., Binn L. N., Marchwicki R. H. Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures. J Clin Microbiol. 1983 May;17(5):834–839. doi: 10.1128/jcm.17.5.834-839.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Liu S., Birch C., Coulepis A., Gust I. Radioimmunofocus assay for detection and quantitation of human rotavirus. J Clin Microbiol. 1984 Sep;20(3):347–350. doi: 10.1128/jcm.20.3.347-350.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pursell A. R., Cole J. R., Jr Procedure for fluorescent-antibody staining of virus-infected cell cultures in plastic plates. J Clin Microbiol. 1976 May;3(5):537–540. doi: 10.1128/jcm.3.5.537-540.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schoepp R. J., Beaty B. J. Titration of dengue viruses by immunofluorescence in microtiter plates. J Clin Microbiol. 1984 Nov;20(5):1017–1019. doi: 10.1128/jcm.20.5.1017-1019.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ward R. L., Knowlton D. R., Pierce M. J. Efficiency of human rotavirus propagation in cell culture. J Clin Microbiol. 1984 Jun;19(6):748–753. doi: 10.1128/jcm.19.6.748-753.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wyatt R. G., James W. D., Bohl E. H., Theil K. W., Saif L. J., Kalica A. R., Greenberg H. B., Kapikian A. Z., Chanock R. M. Human rotavirus type 2: cultivation in vitro. Science. 1980 Jan 11;207(4427):189–191. doi: 10.1126/science.6243190. [DOI] [PubMed] [Google Scholar]