Superoxide flashes: illuminating new insights into cardiac ischemia/reperfusion injury

SUMMARY

Although the mitochondrial permeability transition pore (mPTP) was first discovered almost 30 years ago [1], it did not attract significant research attention until the 1990's when several studies implicated mPTP in apoptosis [2]. Today, the dogma suggests that opening of mPTP is detrimental to the cell and mPTP activation is widely thought to contribute to disease in cancer, neurodegenerative diseases, stroke, muscular dystrophy, and cardiac reperfusion injury [3]. Multiple factors including Ca²⁺, OH⁻, P_i, cyclophilin D, reactive oxygen and nitrogen species (ROS and RNS) trigger mPTP opening [4]. However, whether mPTP activation feeds back to alter mitochondrial ROS generation remains unclear. We recently demonstrated that under normal conditions, individual mitochondria undergo spontaneous transient bursts of quantal superoxide generation, termed “superoxide flashes” [5]. Superoxide flashes are observed in all cell types investigated to date and are triggered by a surprising functional coupling between mPTP activation and electron transport chain (ETC) dependent superoxide production. Additionally, reoxgenation following anoxia leads to uncontrolled superoxide flash genesis in cardiomyocytes. This positive feedback mechanism for mPTP/ETC-dependent ROS generation may drive localized redox signaling in individual mitochondria under physiological conditions, and when left unchecked, contribute to global cellular oxidative stress under pathological conditions in cardiac disease. The mPTP activity-dependent cell life and death determination imposes new challenges and opportunities in the pursuit of therapeutic agents for treating diseases in which oxidative stress has been implicated such as cardiac ischemia-reperfusion injury.

Mitochondrial Permeability Transition Pore as a Drug Target for Attenuating Reperfusion Injury

The crucial role of mPTP in causing cell death has put it as a new drug target for treating diseases across a wide spectrum of organs including heart, liver, nervous system, lung and muscle, as well as cancer (for review, see [6], [7]). In a recent report, administration of the mPTP inhibitor cyclosporine A at the time of percutaneous coronary intervention in 30 patients was found to reduce infarct size greater than that observed with placebo [8]. These clinical data are consistent with numerous basic science studies showing that mPTP activation is a key step in the pathogenesis of ischemia-reperfusion injury [9], [10]. The sequence of events in cardiac ischemia/reperfusion injury begins with lactic acidosis of myocytes during ischemia. Cellular acidosis augments Na⁺/H⁺ exchange activity to move H⁺ out of cells, resulting in intracellular Na⁺ overload. Subsequently, the plasma membrane Na⁺/Ca²⁺ exchanger would operate less effectively in forward mode to transport Ca²⁺ out of the myocyte and more effectively to bring Ca²⁺ into the cell during conditions favoring reverse mode Na⁺/Ca²⁺ exchange, ultimately resulting in myoplasmic Ca²⁺ overload. The increase in myoplasmic Ca²⁺ is then taken up by mitochondria, resulting in a mitochondrial Ca²⁺ overload. During reperfusion, the reintroduction of normal oxygen and H⁺ concentrations rapidly “wakes up” the ETC, which leads to a massive increase in ROS generation. The combination of mitochondrial Ca²⁺ and ROS overload causes the opening of mPTP, release of cytochrome c, and apoptosis/necrosis [11], [10]. Since one of the end points for ischemia/reperfusion injury is the opening of the mPTP, the ability of prior cyclosporine A administration to reduce infarct size is intuitively expected.

Why Other Pharmacological Agents Failed in Ischemia/Reperfusion Clinical Trials

Intriguingly, the above mentioned clinical study with a positive outcome is an exception rather than a norm. Over just the last decade, several clinical trials have been launched using pharmacological interventions designed to reduce acute myocardial infarction during reperfusion injury. The results of most of these studies either failed to show beneficial effects or are considered controversial [12]. These clinical trials include (a) Ca²⁺ channel inhibition using diltiazem and MgSO₄ to decrease cellular Ca²⁺ overload, (b) Na⁺/H⁺ inhibitors such as cariporide to decrease cellular Na⁺ overload, (c) anti-inflammatory and anti-oxidant agents such as fluosol and recombinant human superoxide dismutase to detoxify ROS, and (d) pharmacological agents such as adenosine and volatile anesthetics to preconditioning the heart to better handle reperfusion insult. The key reasons for the discrepancy between the pre-clinical and clinical studies have been discussed extensively in several recent reviews [12], [13], [14]. In many cases, patients in these clinical studies were older and suffering from other complications in addition to ischemic heart disease. As a result these patients were often taking multiple medications, with the pharmacokinetic parameters of each agent, including drug absorption, distribution, and metabolism, being difficult to assess. Another factor which may limit the applicability of these studies is the lack of solid quantitative information regarding the therapeutic index of the drugs used. Therefore, it is likely that in some studies the dose of drug required to elicit a therapeutic response without unacceptable adverse effects was not achieved. Moreover, most of the drugs used in these studies were not targeted to a specific organ (e.g. heart) or a specific organelle (e.g. mitochondria).

Coupling of mPTP to ROS Generation

Our recent findings of mPTP-coupled quantal ROS generation [5], provides new insight into why such treatments lead to divergent outcomes. The major finding of our study is the serendipitous discovery and subsequent characterization of novel transient superoxide producing events, termed “superoxide flashes”. Superoxide flashes are brief bursts of superoxide production within the matrix of single mitochondria in intact quiescent cells. These superoxide flashes result from an unexpected functional coupling between transient openings of the mPTP and ETC activity. Superoxide flashes are found in a wide range of different cells including cardiac myocytes, neurons, neuroendocrine cells, skeletal muscle fibers, and clonal cell lines. While the fundamental properties (magnitude and duration) of superoxide flashes are similar in all cells, flash frequency varies dramatically between cell types. In general, flash frequencies tend to be higher in dividing/proliferative cells and lower in terminally differentiated cells. Importantly, we found that superoxide flash frequency decreases during cardiac hypoxia/anoxia and exhibits a marked rebound increase during early reoxygenation after anoxia.

Yin and Yang of Superoxide Flashes: Physiological and Pathological Significance

Superoxide is the primary reactive oxygen species produced by cells, which means that physiological and pathological reactive oxygen species signaling begins with superoxide production. Thus, just as quantal localized calcium release events (Ca²⁺ sparks) underlie intracellular calcium signaling in the heart [15], superoxide flashes may represent elementary events of ROS production and signaling within the mitochondrial matrix. Physiologically, these stochastic elementary events of mitochondrial superoxide production may regulate redox sensitive processes that are restricted to either within or immediately adjacent to the flash producing mitochondrion. Indeed, recent evidence indicates that an array of cellular functions (e.g. channel/transporter activity, Ca²⁺ spark production, kinase/phosphatase activity, gene expression, etc.) are highly redox sensitive [16]. Left uncontrolled, a high frequency of superoxide flash activity may lead to local, and eventually widespread, cellular damage and thus contribute to the pathogenesis of a variety of different oxidative stress-related disorders.

New Insights into Future Drug Development in Treating Oxidative Stress Mediated Diseases

It is well known that massive openings of the mPTP play a critical role in cell pathology. As shown in our study, pathological permeability transition pore openings and uncontrolled superoxide flash activity contributes to the destructive burst of ROS observed during the early phase of reperfusion. Superoxide flashes may also be of relevance to the long-standing debate regarding the proposed contribution of transient openings of the mPTP within a physiological context. Since superoxide flashes occur relatively infrequently under resting conditions in differentiated cells, superoxide flash activity under these conditions likely reflects infrequent basal mPTP activity that contributes to local physiological ROS signaling. Thus, it is conceivable that clinical trials of drugs designed to block ROS stress resulting from uncontrolled mPTP activity could be detrimental because these agents may also inhibit physiological levels of superoxide generation required for local ROS signaling in healthy tissues. In addition, as the drugs used in these studies are not specifically targeted to mitochondria, the concentrations required to inhibit mitochondrial ROS production are high, and thus, confounded by unavoidable toxicity. Therefore, future related drug development initiatives will need to identify agents that effectively block massive mPTP opening and uncontrolled ROS production, but not basal, physiologically-relevant ROS production. Moreover, since superoxide flashes are triggered by transient openings of the mPTP, superoxide flash activity may potentially be used as both a biomarker of mPTP function/activity and as an index for screening potential therapeutic agents to protect from ischemia-reperfusion injury.

Future Perspective in Health and Disease

Our discovery of quantal superoxide production within the mitochondrial matrix will stimulate future studies of intracellular ROS signaling, mitochondrial functionality and regulation of cell metabolism. First, superoxide flashes constitute elementary events of transient and quantal ROS generation within a single mitochondrion. Previously, researchers believed that mitochondrial ROS production results solely as a minimal byproduct of continuous or constitutive mitochondrial respiration. Second, our discovery of intermittent mitochondrial superoxide producing events reflects a new paradigm of mitochondrial function in living cells, namely that mPTP activity accelerates or resets mitochondrial respiration and stimulates superoxide generation by the ETC. Consequently, mitochondrial metabolic rate may be regulated by external factors that influence mPTP opening. The exact signal(s) that triggers transient mPTP opening and subsequent quantal superoxide flash generation is unknown; an answer to this question will be of great importance since it may help to nail down factors that control cell metabolism, cell function and cell fate under both resting and stress conditions. For now, we believe that low levels of constitutive ROS production during continuous mitochondria respiration is a likely candidate. Finally, at the cellular level, the temporal and spatial integration of quantal superoxide signals provides a mechanism for diversification of ROS signaling in various cell processes. As we've shown in our study, the basal frequency of superoxide flash activity varies significantly between different cell types, which may indicate differences in cell metabolic activity, proliferation rate and functionality. For example, neonatal cardiac myocytes exhibit a much higher superoxide flash frequency than adult cardiac myocytes, consistent with higher metabolic and growth rates in developing myocytes. Taken together, the transient opening of mPTP serves as a code for both cellular redox signaling and metabolic coupling. As indicated in a recent paper by Wallace [17], historically, the etiology of human diseases is mostly attributed to structural (anatomical) and genetic (Mendelian) defects. However, this disease paradigm has come to a bottleneck. The next major leap in biomedicine will reflect a shifting in this paradigm to include energetic and redox defects. Thus, we predict that elucidation of the mechanisms by which mitochondrial superoxide flashes encode elementary events of energy and ROS generation will greatly change the landscape of future biomedical research and drug development.