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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 Jul;14(1):61–66. doi: 10.1128/jcm.14.1.61-66.1981

Preservation and transportation of bacteria by a simple gelatin disk method.

Y Obara, S Yamai, T Nikkawa, Y Shimoda, Y Miyamoto
PMCID: PMC271902  PMID: 7021588

Abstract

A wide range of bacterial species, e.g., Enterobacteriaceae and Neisseria, Streptococcus, Branhamella, Haemophilus, Gemella, Pseudomonas, Flavobacterium, and Bacteroides species, were successfully preserved for 1 to 5 years by our gelatin disk drying method. The beta-lactamase activity of penicillinase-producing Neisseria gonorrhoeae was retained for more than 3 years with this method. Good results were also obtained upon airmailing many strains of N. gonorrhoeae embedded in gelatin disks from Japan to the United States. Neisseria, Branhamella, Gemella, and Haemophilus organisms suspended in the reagent used in the preparation of the gelatin disks could be preserved for 6 to 12 months after freezing the cell suspensions at -20 degrees C. Furthermore, modification of our gelatin disk preservation method made possible the safe long- and short-distance transportation of clinical isolates. Our methods can be used by any small laboratory, since they require only conventional instruments and reagents.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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