Abstract
Eighteen types of media, all of which used charcoal yeast extract medium as a base, were tested for their ability to support the growth of a stock strain of Legionella pneumophila. Fifteen of these contained antimicrobial agents. Five antibiotic-containing media which performed best in growing the stock strain were then tested for the ability to grow L. pneumophila from clinical respiratory tract specimens. One of the media, BMPA alpha, which contained cefamandole, polymyxin B, anisomycin, an organic buffer, and alpha-ketoglutarate, performed from contaminated clinical specimens and, in conjunction with an acid wash treatment, facilitated recovery of the bacterium from potable water.
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Selected References
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- Bopp C. A., Sumner J. W., Morris G. K., Wells J. G. Isolation of Legionella spp. from environmental water samples by low-pH treatment and use of a selective medium. J Clin Microbiol. 1981 Apr;13(4):714–719. doi: 10.1128/jcm.13.4.714-719.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cherry W. B., Pittman B., Harris P. P., Hebert G. A., Thomason B. M., Thacker L., Weaver R. E. Detection of Legionnaires disease bacteria by direct immunofluorescent staining. J Clin Microbiol. 1978 Sep;8(3):329–338. doi: 10.1128/jcm.8.3.329-338.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelstein P. H., Finegold S. M. Use of a semiselective medium to culture Legionella pneumophila from contaminated lung specimens. J Clin Microbiol. 1979 Aug;10(2):141–143. doi: 10.1128/jcm.10.2.141-143.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edelstein P. H., Meyer R. D., Finegold S. M. Laboratory diagnosis of Legionnaires' disease. Am Rev Respir Dis. 1980 Feb;121(2):317–327. doi: 10.1164/arrd.1980.121.2.317. [DOI] [PubMed] [Google Scholar]
- Feeley J. C., Gibson R. J., Gorman G. W., Langford N. C., Rasheed J. K., Mackel D. C., Baine W. B. Charcoal-yeast extract agar: primary isolation medium for Legionella pneumophila. J Clin Microbiol. 1979 Oct;10(4):437–441. doi: 10.1128/jcm.10.4.437-441.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meyer R. D., Edelstein P. H., Kirby B. D., Louie M. H., Mulligan M. E., Morgenstein A. A., Finegold S. M. Legionnaires' disease: unusual clinical and laboratory features. Ann Intern Med. 1980 Aug;93(2):240–243. doi: 10.7326/0003-4819-93-2-240. [DOI] [PubMed] [Google Scholar]
- Pasculle A. W., Feeley J. C., Gibson R. J., Cordes L. G., Myerowitz R. L., Patton C. M., Gorman G. W., Carmack C. L., Ezzell J. W., Dowling J. N. Pittsburgh pneumonia agent: direct isolation from human lung tissue. J Infect Dis. 1980 Jun;141(6):727–732. doi: 10.1093/infdis/141.6.727. [DOI] [PubMed] [Google Scholar]
- Smalley D. L., Jaquess P. A., Layne J. S. Selenium-enriched medium for Legionella pneumophila. J Clin Microbiol. 1980 Jul;12(1):32–34. doi: 10.1128/jcm.12.1.32-34.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thorpe T. C., Miller R. D. Negative enrichment procedure for isolation of Legionella pneumophila from seeded cooling tower water. Appl Environ Microbiol. 1980 Oct;40(4):849–851. doi: 10.1128/aem.40.4.849-851.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]