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. 1981 Sep;14(3):318–321. doi: 10.1128/jcm.14.3.318-321.1981

Clinical evaluation of the rapid carbohydrate degradation microtube method for identification of Neisseria species.

S M Qadri, N Parker
PMCID: PMC271961  PMID: 7026601

Abstract

The rapid carbohydrate degradation (Carr Microbiologicals Wichita, Kans.) microtube method is a new test system designed for the identification of Neisseria spp. The system consists of four microtubes containing different carbohydrates in a peptone-Bitone basal medium. This method was evaluated for accuracy and speed in identifying species of Neisseria. Of the 386 clinical isolates used in this study, 98.4% were correctly identified to species level in 4 h with the rapid carbohydrate degradation system; parallel testing of the same isolates with conventional cystine-tryptic agar resulted in 96.1% accuracy in 48 h.

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Selected References

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  1. Anand C. M., Kadis E. M. Evaluation of the Phadebact Gonococcus Test for confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1980 Jul;12(1):15–17. doi: 10.1128/jcm.12.1.15-17.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Appelbaum P. C., Lawrence R. B. Comparison of three methods for identification of pathogenic Neisseria species. J Clin Microbiol. 1979 May;9(5):598–600. doi: 10.1128/jcm.9.5.598-600.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Barnham M., Glynn A. A. Identification of clinical isolates of Neisseria gonorrhoeae by a coagglutination test. J Clin Pathol. 1978 Feb;31(2):189–193. doi: 10.1136/jcp.31.2.189. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bawdon R. E., Juni E., Britt E. M. Identification of Neisseria gonorrhoeae by genetic transformation: a clinical laboratory evaluation. J Clin Microbiol. 1977 Jan;5(1):108–109. doi: 10.1128/jcm.5.1.108-109.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown W. J. A comparison of three fermentation methods for the confirmation of Neisseria gonorrhoeae. Health Lab Sci. 1976 Jan;13(1):54–58. [PubMed] [Google Scholar]
  6. Brown W. J. Modification of the rapid fermentation test for Neisseria gonorrhoeae. Appl Microbiol. 1974 Jun;27(6):1027–1030. doi: 10.1128/am.27.6.1027-1030.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Cox F. R., Cox M. E., Martin J. R., Stoer U. H. Radiometric detection of carbohydrate catabolism by pathogenic Neisseria. J Clin Microbiol. 1977 Sep;6(3):310–311. doi: 10.1128/jcm.6.3.310-311.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. D'Amato R. F., Eriquez L. A., Tomfohrde K. M., Singerman E. Rapid identification of Neisseria gonorrhoeae and Neisseria meningitidis by using enzymatic profiles. J Clin Microbiol. 1978 Jan;7(1):77–81. doi: 10.1128/jcm.7.1.77-81.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Danielsson D., Kronvall G. Slide agglutination method for the serological identification of Neisseria gonorrhoeae with anti-gonococcal antibodies adsorbed to protein A-containing staphylococci. Appl Microbiol. 1974 Feb;27(2):368–374. doi: 10.1128/am.27.2.368-374.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Devine L. F., Hagerman C. R. Relationship of Serogroups of Neisseria meningitidis I. Microagglutination, Gel Diffusion, and Slide Agglutination Studies of Meningococcal Antisera Before and After Absorption with RAS-10 Strain of Meningococci. Infect Immun. 1970 Mar;1(3):226–231. doi: 10.1128/iai.1.3.226-231.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Faur Y. C., Weisburd M. H., Wilson M. E. Carbohydrate fermentation plate medium for confirmation of Neisseria species. J Clin Microbiol. 1975 Mar;1(3):294–297. doi: 10.1128/jcm.1.3.294-297.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Flynn J., Waitkins S. A. A serum-free medium for testing fermentation reactions in Neisseria gonorrhoeae. J Clin Pathol. 1972 Jun;25(6):525–527. doi: 10.1136/jcp.25.6.525. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Hampton K. D., Stallings R. A., Wasilauskas B. L. Comparison of a slide coagglutination technique with the Minitek system for confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1979 Sep;10(3):290–292. doi: 10.1128/jcm.10.3.290-292.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Kellogg D. S., Jr, Turner E. M. Rapid fermentation confirmation of Neisseria gonorrhoeae. Appl Microbiol. 1973 Apr;25(4):550–552. doi: 10.1128/am.25.4.550-552.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Morse C. D., Brooks J. B., Kellogg D. S., Jr Electron capture gas chromatographic detection of acethylmethylcarbinol produced by neisseria gonorrhoeae. J Clin Microbiol. 1976 Jan;3(1):34–41. doi: 10.1128/jcm.3.1.34-41.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morse S. A., Bartenstein L. Adaptation of the Minitek system for the rapid identification of Neisseria gonorrhoeae. J Clin Microbiol. 1976 Jan;3(1):8–13. doi: 10.1128/jcm.3.1.8-13.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Pizzuto D. J., Washington J. A., 2nd Evaluation of rapid carbohydrate degradation tests for identification of pathogenic Neisseria. J Clin Microbiol. 1980 Apr;11(4):394–397. doi: 10.1128/jcm.11.4.394-397.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Reddick A. A simple carbohydrate fermentation test for identification of the pathogenic Neisseria. J Clin Microbiol. 1975 Jul;2(1):72–73. doi: 10.1128/jcm.2.1.72-73.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Reyn A. Recent developments in the laboratory diagnosis of gonococcal infections. Bull World Health Organ. 1969;40(2):245–255. [PMC free article] [PubMed] [Google Scholar]
  20. Schaefer R. L., Keller K. F., Doyle R. J. Lectins in diagnostic microbiology: use of wheat germ agglutinin for laboratory identification of Neisseria gonorrhoeae. J Clin Microbiol. 1979 Nov;10(5):669–672. doi: 10.1128/jcm.10.5.669-672.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Shtibel R., Toma S. Neisseria gonorrhoeae: evaluation of some methods used for carbohydrate utilization. Can J Microbiol. 1978 Feb;24(2):177–181. doi: 10.1139/m78-030. [DOI] [PubMed] [Google Scholar]
  22. Slifkin M., Pouchet G. R. Rapid carbohydrate fermentation test for confirmation of the pathogenic Neisseria using a Ba(OH)2 indicator. J Clin Microbiol. 1977 Jan;5(1):15–19. doi: 10.1128/jcm.5.1.15-19.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Strauss R. R., Holderbach J., Friedman H. Comparison of a radiometric procedure with conventional methods for identification of Neisseria. J Clin Microbiol. 1978 May;7(5):419–422. doi: 10.1128/jcm.7.5.419-422.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Wallace R., Ashton F., Charron F., Diena B. B. An improved sugar fermentation technique for the confirmation of Neisseria gonorrhoeae. Can J Public Health. 1975 May-Jun;66(3):251–252. [PubMed] [Google Scholar]
  25. Yong D. C., Prytula A. Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae. J Clin Microbiol. 1978 Dec;8(6):643–647. doi: 10.1128/jcm.8.6.643-647.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Young H., Paterson I. C., McDonald D. R. Rapid carbohydrate utilization test for the identification of Neisseria gonorrhoeae. Br J Vener Dis. 1976 Jun;52(3):172–175. doi: 10.1136/sti.52.3.172. [DOI] [PMC free article] [PubMed] [Google Scholar]

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