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. 1991 Jun;29(6):1255–1257. doi: 10.1128/jcm.29.6.1255-1257.1991

Synthetic oligonucleotide probes differentiate respiratory syncytial virus subgroups in a nucleic acid hybridization assay.

W M Sullender 1, G W Wertz 1
PMCID: PMC271971  PMID: 1864945

Abstract

A new approach to respiratory syncytial virus subgroup differentiation has been developed on the basis of the hybridization of subgroup-specific synthetic oligonucleotides with viral mRNA. Two oligonucleotide probes were designed from the nucleotide sequences of an A and a B subgroup respiratory syncytial virus glycoprotein G gene. All 28 virus isolates tested were correctly classified by subgroup with these probes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson L. J., Hierholzer J. C., Tsou C., Hendry R. M., Fernie B. F., Stone Y., McIntosh K. Antigenic characterization of respiratory syncytial virus strains with monoclonal antibodies. J Infect Dis. 1985 Apr;151(4):626–633. doi: 10.1093/infdis/151.4.626. [DOI] [PubMed] [Google Scholar]
  2. Hendry R. M., Burns J. C., Walsh E. E., Graham B. S., Wright P. F., Hemming V. G., Rodriguez W. J., Kim H. W., Prince G. A., McIntosh K. Strain-specific serum antibody responses in infants undergoing primary infection with respiratory syncytial virus. J Infect Dis. 1988 Apr;157(4):640–647. doi: 10.1093/infdis/157.4.640. [DOI] [PubMed] [Google Scholar]
  3. Hendry R. M., Pierik L. T., McIntosh K. Prevalence of respiratory syncytial virus subgroups over six consecutive outbreaks: 1981-1987. J Infect Dis. 1989 Aug;160(2):185–190. doi: 10.1093/infdis/160.2.185. [DOI] [PubMed] [Google Scholar]
  4. Hendry R. M., Talis A. L., Godfrey E., Anderson L. J., Fernie B. F., McIntosh K. Concurrent circulation of antigenically distinct strains of respiratory syncytial virus during community outbreaks. J Infect Dis. 1986 Feb;153(2):291–297. doi: 10.1093/infdis/153.2.291. [DOI] [PubMed] [Google Scholar]
  5. Johnson P. R., Collins P. L. The A and B subgroups of human respiratory syncytial virus: comparison of intergenic and gene-overlap sequences. J Gen Virol. 1988 Nov;69(Pt 11):2901–2906. doi: 10.1099/0022-1317-69-11-2901. [DOI] [PubMed] [Google Scholar]
  6. Johnson P. R., Jr, Olmsted R. A., Prince G. A., Murphy B. R., Alling D. W., Walsh E. E., Collins P. L. Antigenic relatedness between glycoproteins of human respiratory syncytial virus subgroups A and B: evaluation of the contributions of F and G glycoproteins to immunity. J Virol. 1987 Oct;61(10):3163–3166. doi: 10.1128/jvi.61.10.3163-3166.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Johnson P. R., Spriggs M. K., Olmsted R. A., Collins P. L. The G glycoprotein of human respiratory syncytial viruses of subgroups A and B: extensive sequence divergence between antigenically related proteins. Proc Natl Acad Sci U S A. 1987 Aug;84(16):5625–5629. doi: 10.1073/pnas.84.16.5625. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Morgan L. A., Routledge E. G., Willcocks M. M., Samson A. C., Scott R., Toms G. L. Strain variation of respiratory syncytial virus. J Gen Virol. 1987 Nov;68(Pt 11):2781–2788. doi: 10.1099/0022-1317-68-11-2781. [DOI] [PubMed] [Google Scholar]
  9. Mufson M. A., Belshe R. B., Orvell C., Norrby E. Subgroup characteristics of respiratory syncytial virus strains recovered from children with two consecutive infections. J Clin Microbiol. 1987 Aug;25(8):1535–1539. doi: 10.1128/jcm.25.8.1535-1539.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mufson M. A., Orvell C., Rafnar B., Norrby E. Two distinct subtypes of human respiratory syncytial virus. J Gen Virol. 1985 Oct;66(Pt 10):2111–2124. doi: 10.1099/0022-1317-66-10-2111. [DOI] [PubMed] [Google Scholar]
  11. Paeratakul U., De Stasio P. R., Taylor M. W. A fast and sensitive method for detecting specific viral RNA in mammalian cells. J Virol. 1988 Apr;62(4):1132–1135. doi: 10.1128/jvi.62.4.1132-1135.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Peterson E. M., Aarnaes S. L., Bryan R. N., Ruth J. L., de la Maza L. M. Typing of herpes simplex virus with synthetic DNA probes. J Infect Dis. 1986 Apr;153(4):757–762. doi: 10.1093/infdis/153.4.757. [DOI] [PubMed] [Google Scholar]
  13. Sethabutr O., Unicomb L. E., Holmes I. H., Taylor D. N., Bishop R. F., Echeverria P. Serotyping of human group A rotavirus with oligonucleotide probes. J Infect Dis. 1990 Aug;162(2):368–372. doi: 10.1093/infdis/162.2.368. [DOI] [PubMed] [Google Scholar]
  14. Stott E. J., Taylor G., Ball L. A., Anderson K., Young K. K., King A. M., Wertz G. W. Immune and histopathological responses in animals vaccinated with recombinant vaccinia viruses that express individual genes of human respiratory syncytial virus. J Virol. 1987 Dec;61(12):3855–3861. doi: 10.1128/jvi.61.12.3855-3861.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Sullender W. M., Anderson K., Wertz G. W. The respiratory syncytial virus subgroup B attachment glycoprotein: analysis of sequence, expression from a recombinant vector, and evaluation as an immunogen against homologous and heterologous subgroup virus challenge. Virology. 1990 Sep;178(1):195–203. doi: 10.1016/0042-6822(90)90394-7. [DOI] [PubMed] [Google Scholar]
  16. Sullender W. M., Anderson L. J., Anderson K., Wertz G. W. Differentiation of respiratory syncytial virus subgroups with cDNA probes in a nucleic acid hybridization assay. J Clin Microbiol. 1990 Aug;28(8):1683–1687. doi: 10.1128/jcm.28.8.1683-1687.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Wertz G. W., Collins P. L., Huang Y., Gruber C., Levine S., Ball L. A. Nucleotide sequence of the G protein gene of human respiratory syncytial virus reveals an unusual type of viral membrane protein. Proc Natl Acad Sci U S A. 1985 Jun;82(12):4075–4079. doi: 10.1073/pnas.82.12.4075. [DOI] [PMC free article] [PubMed] [Google Scholar]

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