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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1991 Jun;29(6):1258–1259. doi: 10.1128/jcm.29.6.1258-1259.1991

Comparison of the new MicroScan Pos MIC Type 6 panel and AMS-Vitek Gram Positive Susceptibility Card (GPS-TA) for detection of high-level aminoglycoside resistance in Enterococcus species.

S Szeto 1, M Louie 1, D E Low 1, M Patel 1, A E Simor 1
PMCID: PMC271972  PMID: 1907610

Abstract

We compared the MicroScan Pos MIC Type 6 panel and AMS-Vitek Gram Positive Susceptibility Card (GPS-TA) to agar dilution screen plates for the detection of high-level aminoglycoside resistance in 182 enterococcal isolates. The specificity of the two commercial systems was 100%, with the exception of one susceptible isolate found to be streptomycin resistant by the Vitek system. The MicroScan and Vitek systems had comparable sensitivities for the detection of gentamicin resistance (90 and 95% respectively) and streptomycin resistance (85 and 78%, respectively). These results suggest that screening tests such as agar dilution screen plates, broth dilution, or disk diffusion should continue to be used to detect high-level gentamicin and streptomycin resistance.

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Selected References

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  1. Calderwood S. A., Wennersten C., Moellering R. C., Jr, Kunz L. J., Krogstad D. J. Resistance to six aminoglycosidic aminocyclitol antibiotics among enterococci: prevalence, evolution, and relationship to synergism with penicillin. Antimicrob Agents Chemother. 1977 Sep;12(3):401–405. doi: 10.1128/aac.12.3.401. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Fuller S. A., Low D. E., Simor A. E. Evaluation of a commercial microtiter system (MicroScan) using both frozen and freeze-dried panels for detection of high-level aminoglycoside resistance in Enterococcus spp. J Clin Microbiol. 1990 May;28(5):1051–1053. doi: 10.1128/jcm.28.5.1051-1053.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Mederski-Samoraj B. D., Murray B. E. High-level resistance to gentamicin in clinical isolates of enterococci. J Infect Dis. 1983 Apr;147(4):751–757. doi: 10.1093/infdis/147.4.751. [DOI] [PubMed] [Google Scholar]
  4. Moellering R. C., Jr, Wennersten C., Weinberg A. N. Studies on antibiotic synergism against enterococci. I. Bacteriologic studies. J Lab Clin Med. 1971 May;77(5):821–828. [PubMed] [Google Scholar]
  5. Murray B. E. The life and times of the Enterococcus. Clin Microbiol Rev. 1990 Jan;3(1):46–65. doi: 10.1128/cmr.3.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Patterson J. E., Zervos M. J. High-level gentamicin resistance in Enterococcus: microbiology, genetic basis, and epidemiology. Rev Infect Dis. 1990 Jul-Aug;12(4):644–652. doi: 10.1093/clinids/12.4.644. [DOI] [PubMed] [Google Scholar]
  7. Rosenthal S. L., Freundlich L. F. An aminoglycoside disk sensitivity test for use with enterococci. J Antimicrob Chemother. 1982 Nov;10(5):459–462. doi: 10.1093/jac/10.5.459. [DOI] [PubMed] [Google Scholar]
  8. Sahm D. F., Torres C. Effects of medium and inoculum variations on screening for high-level aminoglycoside resistance in Enterococcus faecalis. J Clin Microbiol. 1988 Feb;26(2):250–256. doi: 10.1128/jcm.26.2.250-256.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sahm D. F., Torres C. High-content aminoglycoside disks for determining aminoglycoside-penicillin synergy against Enterococcus faecalis. J Clin Microbiol. 1988 Feb;26(2):257–260. doi: 10.1128/jcm.26.2.257-260.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Spiegel C. A. Laboratory detection of high-level aminoglycoside-aminocyclitol resistance in Enterococcus spp. J Clin Microbiol. 1988 Nov;26(11):2270–2274. doi: 10.1128/jcm.26.11.2270-2274.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Watanakunakorn C. The prevalence of high-level aminoglycoside resistance among enterococci isolated from blood cultures during 1980-1988. J Antimicrob Chemother. 1989 Jul;24(1):63–68. doi: 10.1093/jac/24.1.63. [DOI] [PubMed] [Google Scholar]
  12. Zervos M. J., Patterson J. E., Edberg S., Pierson C., Kauffman C. A., Mikesell T. S., Schaberg D. R. Single-concentration broth microdilution test for detection of high-level aminoglycoside resistance in enterococci. J Clin Microbiol. 1987 Dec;25(12):2443–2444. doi: 10.1128/jcm.25.12.2443-2444.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]

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