Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1981 Oct;14(4):376–382. doi: 10.1128/jcm.14.4.376-382.1981

Cellular fatty acid and soluble protein composition of Actinobacillus actinomycetemcomitans and related organisms.

D A Calhoon, W R Mayberry, J Slots
PMCID: PMC271987  PMID: 7287893

Abstract

The cellular fatty acid and protein content of twenty-five representative strains of Actinobacillus actinomycetecomitans isolated from juvenile and adult periodontitis patients was compared to that of 15 reference strains of oral and nonoral Actinobacillus species and Haemophilus aphrophilus. Trimethylsilyl derivatives of the fatty acid methyl esters were analyzed by gas-liquid chromatography. The predominant fatty acids of all 40 strains examined were 14:0, 3-OH 14:0, 16 delta, and 16:0. Actinobacillus seminis (ATCC 15768) was unlike the other strains examined because of a greater amount of 14:0 detected. The soluble protein analysis using polyacrylamide gel electrophoresis revealed that A. actinomycetemcomitans, H. aphrophilus, and nonoral Actinobacillus species possessed distinct protein profiles attesting to the validity of separating these organisms into different species. Established biotypes of A. actinomycetemcomitans could not be differentiated on the basis of fatty acid or protein profiles.

Full text

PDF
376

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABEL K., DESCHMERTZING H., PETERSON J. I. CLASSIFICATION OF MICROORGANISMS BY ANALYSIS OF CHEMICAL COMPOSITION. I. FEASIBILITY OF UTILIZING GAS CHROMATOGRAPHY. J Bacteriol. 1963 May;85:1039–1044. doi: 10.1128/jb.85.5.1039-1044.1963. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Braunthal S. D., Holt S. C., Tanner A. C., Socransky S. S. Cellular fatty acid composition of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus. J Clin Microbiol. 1980 Jun;11(6):625–630. doi: 10.1128/jcm.11.6.625-630.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gross C. S., Ferguson D. A., Jr, Cummins C. S. Electrophoretic protein patterns and enzyme mobilities in anaerobic coryneforms. Appl Environ Microbiol. 1978 Jun;35(6):1102–1108. doi: 10.1128/aem.35.6.1102-1108.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Jantzen E., Berdal B. P., Omland T. Cellular fatty acid composition of Haemophilus species, Pasteurella multocida, Actinobacillus Actinomycetemcomitans and Haemophilus vaginalis (Corynebacterium vaginale). Acta Pathol Microbiol Scand B. 1980 Apr;88(2):89–93. doi: 10.1111/j.1699-0463.1980.tb02611.x. [DOI] [PubMed] [Google Scholar]
  5. KING E. O., TATUM H. W. Actinobacillus actinomycetemcomitans and Hemophilus aphrophilus. J Infect Dis. 1962 Sep-Oct;111:85–94. doi: 10.1093/infdis/111.2.85. [DOI] [PubMed] [Google Scholar]
  6. Kiley P., Holt S. C. Characterization of the lipopolysaccharide from Actinobacillus actinomycetemcomitans Y4 and N27. Infect Immun. 1980 Dec;30(3):862–873. doi: 10.1128/iai.30.3.862-873.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol. 1976 Mar;93(1):9–62. doi: 10.1099/00221287-93-1-9. [DOI] [PubMed] [Google Scholar]
  8. Mayberry W. R. Hydroxy fatty acids in Bacteroides species: D-(--)-3-hydroxy-15-methylhexadecanoate and its homologs. J Bacteriol. 1980 Aug;143(2):582–587. doi: 10.1128/jb.143.2.582-587.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mayberry W. R., Smith P. F., Langworthy T. A. Heptose-containing pentaglycosyl diglyceride among the lipids of Acholeplasma modicum. J Bacteriol. 1974 Jun;118(3):898–904. doi: 10.1128/jb.118.3.898-904.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Moore W. E., Hash D. E., Holdeman L. V., Cato E. P. Polyacrylamide slab gel electrophoresis of soluble proteins for studies of bacterial floras. Appl Environ Microbiol. 1980 Apr;39(4):900–907. doi: 10.1128/aem.39.4.900-907.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pulverer G., Ko H. L. Actinobacillus actinomycetem-comitans: fermentative capabilities of 140 strains. Appl Microbiol. 1970 Nov;20(5):693–695. doi: 10.1128/am.20.5.693-695.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Slots J., Reynolds H. S., Genco R. J. Actinobacillus actinomycetemcomitans in human periodontal disease: a cross-sectional microbiological investigation. Infect Immun. 1980 Sep;29(3):1013–1020. doi: 10.1128/iai.29.3.1013-1020.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES